Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

Abstract Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. Keywords : Anthocyanin, flavonoid, Eutrema salsugineum , R2R3 MYB transcription factor, Es MYB90, transcriptional regulation, anthocyanin biosynthesis genes.

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v3 ◽

2020 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

R2r3 Myb

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum . Result: Here we report the identification of an important anthocyanin biosynthesis regulator Es MYB90 from Eutrema salsugineum , which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that Es MYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by Es MYB90 in 35S : EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that Es MYB90 promoted expression of early ( PAL , CHS , and CHI ) and late ( DFR , ANS , and UFGT ) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S : EsMYB90 tobacco transgenic plants. Conclusions: Our results indicated that Es MYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants. Keywords : Anthocyanin, flavonoid, Eutrema salsugineum , R2R3 MYB transcription factor, Es MYB90, transcriptional regulation, anthocyanin biosynthesis genes.

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v1 ◽

2019 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a novel MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v6 ◽

2020 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v5 ◽

2020 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

Anthocyanin Production

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Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

10.21203/rs.2.18301/v2 ◽

2020 ◽

Author(s):

Yuting Qi ◽

Caihong Gu ◽

Xingjun Wang ◽

Shiqing Gao ◽

Changsheng Li ◽

...

Keyword(s):

Transcription Factor ◽

Transgenic Plants ◽

Anthocyanin Biosynthesis ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Anthocyanin Synthesis ◽

Eutrema Salsugineum ◽

Tobacco Transgenic Plants ◽

Myb Genes ◽

Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

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Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

Functional Plant Biology ◽

10.1071/fp19317 ◽

2020 ◽

Vol 47 (5) ◽

pp. 454

Author(s):

Jian Li ◽

Tian Chen ◽

Fengzhen Huang ◽

Penghui Dai ◽

Fuxiang Cao ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factor ◽

Transgenic Plants ◽

Ectopic Expression ◽

Myb Transcription Factor ◽

Seed Abortion ◽

Transgenic Seeds ◽

Genes Encoding ◽

Colour Changes ◽

R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

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Ectopic Expression of Multiple Chrysanthemum (Chrysanthemum × morifolium) R2R3-MYB Transcription Factor Genes Regulates Anthocyanin Accumulation in Tobacco

Genes ◽

10.3390/genes10100777 ◽

2019 ◽

Vol 10 (10) ◽

pp. 777 ◽

Cited By ~ 3

Author(s):

Yan Hong ◽

Mengling Li ◽

Silan Dai

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Functional Divergence ◽

Ectopic Expression ◽

Chrysanthemum Morifolium ◽

Amino Acid Sequences ◽

Regulatory Function ◽

Myb Transcription Factor ◽

Anthocyanin Accumulation ◽

R2r3 Myb

The generation of chrysanthemum (Chrysanthemum × morifolium) flower color is mainly attributed to the accumulation of anthocyanins. In the anthocyanin biosynthetic pathway in chrysanthemum, although all of the structural genes have been cloned, the regulatory function of R2R3-MYB transcription factor (TF) genes, which play a crucial role in determining anthocyanin accumulation in many ornamental crops, still remains unclear. In our previous study, four light-induced R2R3-MYB TF genes in chrysanthemum were identified using transcriptomic sequencing. In the present study, we further investigated the regulatory functions of these genes via phylogenetic and alignment analyses of amino acid sequences, which were subsequently verified by phenotypic, pigmental, and structural gene expression analyses in transgenic tobacco lines. As revealed by phylogenetic and alignment analyses, CmMYB4 and CmMYB5 were phenylpropanoid and flavonoid repressor R2R3-MYB genes, respectively, while CmMYB6 was an activator of anthocyanin biosynthesis, and CmMYB7 was involved in regulating flavonol biosynthesis. Compared with wild-type plants, the relative anthocyanin contents in the 35S:CmMYB4 and 35S:CmMYB5 tobacco lines significantly decreased (p < 0.05), while for 35S:CmMYB6 and 35S:CmMYB7, the opposite result was obtained. Both in the 35S:CmMYB4 and 35S:CmMYB5 lines, the relative expression of several anthocyanin biosynthetic genes in tobacco was significantly downregulated (p < 0.05); on the contrary, several genes were upregulated in the 35S:CmMYB6 and 35S:CmMYB7 lines. These results indicate that CmMYB4 and CmMYB5 negatively regulate anthocyanin biosynthesis in chrysanthemum, while CmMYB6 and CmMYB7 play a positive role, which will aid in understanding the complex mechanism regulating floral pigmentation in chrysanthemum and the functional divergence of the R2R3-MYB gene family in higher plants.

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