scholarly journals Effects of Nitrogen Input on Community Structure of the Denitrifying Bacteria with Nitrous Oxide Reductase Gene (nosZ I): A Long-Term Pond Experiment

2021 ◽  
Author(s):  
Jing Zhou ◽  
Yong Kong ◽  
Mengmeng Wu ◽  
Fengyue Shu ◽  
Haijun Wang ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Bacterial Communities ◽  
Denitrifying Bacteria ◽  
Nitrous Oxide Reductase ◽  
Public Attention ◽  
Reductase Gene ◽  
N Input ◽  
Denitrifying Microorganisms ◽  
N Loading

Abstract Excessive nitrogen (N) input is an important factor influencing aquatic ecosystems and has received increasing public attention in the past decades. It remains unclear, however, how N input affects the denitrifying bacterial communities that play a key role in regulating N cycles in various ecosystems. To test our hypothesis – that the abundance and biodiversity of denitrifying bacterial communities decrease with increasing N – we compared the abundance and composition of denitrifying bacteria having nitrous oxide reductase gene (nosZ I) from sediments (0-20 cm) in five experimental ponds with different nitrogen fertilization treatment (TN10, TN20, TN30, TN40, TN50) using quantitative PCR and pyrosequencing techniques. We found that: 1) N addition significantly decreased nosZ I gene abundance, 2) the Invsimpson and Shannon indices (reflecting biodiversity) first increased significantly along with the increasing N loading in TN10~TN40 followed by a decrease in TN50, 3) the beta diversity of the nosZ I denitrifier was clustered into three groups along the TN concentration levels: Cluster I (TN50), Cluster II (TN40), and Cluster III (TN10-TN30), 4) the proportions of Alphaproteobacteria and Betaproteobacteria in the high-N treatment (TN50) were significantly lower than in the lower N treatments (TN10-TN30). 5). The TN concentration was the most important factor driving the alteration of denitrifying bacteria assemblages. Our findings shed new light on the response of denitrification-related bacteria to long-term N loading at pond scale and on the response of denitrifying microorganisms to N pollution.

scholarly journals Variation in the nitrous oxide reductase gene (nosZ)-denitrifying bacterial community in different primary succession stages in the Hailuogou Glacier retreat area, China

2019 ◽  
Author(s):  
Yan Bai ◽  
Xiying Huang ◽  
Xiangrui Zhou ◽  
Quanju Xiang ◽  
Ke Zhao ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Bacterial Community ◽  
Soil Properties ◽  
Bacterial Communities ◽  
Redundancy Analysis ◽  
Primary Succession ◽  
Available Phosphorus ◽  
Nitrous Oxide Reductase ◽  
Reductase Gene ◽  
Successional Stages

Background: The Hailuogou Glacier in the Gongga Mountain region (SW China), on the southeastern edge of the Tibetan Plateau, is well known for its low-elevation modern glaciers. Since the end of the Little Ice Age (LIA), the Hailuogou Glacier has retreated continuously due to global warming, primary vegetation succession and soil chronosequence have developed in this retreat area. The retreated area of Hailuogou Glacier has not been strongly disturbed by human activities, thus it is an ideal models for exploring the biological colonization of nitrogen in the primary successional stages of ecosystem. The nosZ gene encodes the catalytic center of nitrous oxide reductase and is an ideal molecular marker in studying the variation in the denitrifying bacterial community. Methods: Soil properties as well as abundance and composition of the denitrifying bacterial community were determined via chemical analysis, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment length polymorphism (T-RFLP), respectively. The relationships between the nosZ denitrifying bacterial community and soil properties were determined using redundancy analysis (RDA). Soil properties, potential denitrify activity (PDA), and the nitrous oxide reductase gene (nosZ)-denitrifying bacterial communities significantly differed among successional stages. Results: Soil properties, potential denitrify activity (PDA), and the nitrous oxide reductase gene (nosZ)-denitrifying bacterial communities significantly differed among successional stages. Soil pH in the topsoil decreased from 8.42 to 7.19 in the course of primary succession, while soil organic carbon (SOC) and total nitrogen (TN) gradually increased with primary succession. Available phosphorus (AP) and available potassium (AK), as well as potential denitrify activity (PDA), increased gradually and peaked at the 40-year-old site. The abundance of the nosZ denitrifying bacterial community followed a similar trend. The variation in the denitrifying community composition was complex; Mesorhizobium dominated the soil in the early successional stages (0-20 years) and in the mature phase (60 years), with a relative abundance greater than 55%. Brachybacterium was increased in the 40-year-old site, with a relative abundance of 62.74%, while Azospirillum dominated the early successional stages (0-20 years). Redundancy analysis (RDA) showed that the nosZ denitrifying bacterial community correlated with soil available phosphorus and available potassium levels (P < 0.01).

scholarly journals Variation in the nitrous oxide reductase gene (nosZ)-denitrifying bacterial community in different primary succession stages in the Hailuogou Glacier retreat area, China

2019 ◽  
Author(s):  
Yan Bai ◽  
Xiying Huang ◽  
Xiangrui Zhou ◽  
Quanju Xiang ◽  
Ke Zhao ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Bacterial Community ◽  
Soil Properties ◽  
Bacterial Communities ◽  
Redundancy Analysis ◽  
Primary Succession ◽  
Available Phosphorus ◽  
Nitrous Oxide Reductase ◽  
Reductase Gene ◽  
Successional Stages

Background: The Hailuogou Glacier in the Gongga Mountain region (SW China), on the southeastern edge of the Tibetan Plateau, is well known for its low-elevation modern glaciers. Since the end of the Little Ice Age (LIA), the Hailuogou Glacier has retreated continuously due to global warming, primary vegetation succession and soil chronosequence have developed in this retreat area. The retreated area of Hailuogou Glacier has not been strongly disturbed by human activities, thus it is an ideal models for exploring the biological colonization of nitrogen in the primary successional stages of ecosystem. The nosZ gene encodes the catalytic center of nitrous oxide reductase and is an ideal molecular marker in studying the variation in the denitrifying bacterial community. Methods: Soil properties as well as abundance and composition of the denitrifying bacterial community were determined via chemical analysis, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment length polymorphism (T-RFLP), respectively. The relationships between the nosZ denitrifying bacterial community and soil properties were determined using redundancy analysis (RDA). Soil properties, potential denitrify activity (PDA), and the nitrous oxide reductase gene (nosZ)-denitrifying bacterial communities significantly differed among successional stages. Results: Soil properties, potential denitrify activity (PDA), and the nitrous oxide reductase gene (nosZ)-denitrifying bacterial communities significantly differed among successional stages. Soil pH in the topsoil decreased from 8.42 to 7.19 in the course of primary succession, while soil organic carbon (SOC) and total nitrogen (TN) gradually increased with primary succession. Available phosphorus (AP) and available potassium (AK), as well as potential denitrify activity (PDA), increased gradually and peaked at the 40-year-old site. The abundance of the nosZ denitrifying bacterial community followed a similar trend. The variation in the denitrifying community composition was complex; Mesorhizobium dominated the soil in the early successional stages (0-20 years) and in the mature phase (60 years), with a relative abundance greater than 55%. Brachybacterium was increased in the 40-year-old site, with a relative abundance of 62.74%, while Azospirillum dominated the early successional stages (0-20 years). Redundancy analysis (RDA) showed that the nosZ denitrifying bacterial community correlated with soil available phosphorus and available potassium levels (P < 0.01).

Absence of the Nitrous Oxide Reductase Gene Cluster in Commercial Alfalfa Inoculants Is Probably Due to the Extensive Loss of Genes During Rhizobial Domestication

2018 ◽  
Vol 76 (2) ◽  
pp. 299-302 ◽  
Author(s):  
Silvina Brambilla ◽  
Romina Frare ◽  
Gabriela Soto ◽  
Cintia Jozefkowicz ◽  
Nicolás Ayub
Keyword(s):  
Nitrous Oxide ◽  
Gene Cluster ◽  
Nitrous Oxide Reductase ◽  
Reductase Gene

Nitrous oxide reductase gene (nosZ) and N2O reduction along the littoral gradient of a eutrophic freshwater lake

2013 ◽  
Vol 25 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Chaoxu Wang ◽  
Guibing Zhu ◽  
Yu Wang ◽  
Shanyun Wang ◽  
Chengqing Yin
Keyword(s):  
Nitrous Oxide ◽  
Freshwater Lake ◽  
Nitrous Oxide Reductase ◽  
N2o Reduction ◽  
Reductase Gene

scholarly journals Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils

2012 ◽  
Vol 109 (48) ◽  
pp. 19709-19714 ◽  
Author(s):  
R. A. Sanford ◽  
D. D. Wagner ◽  
Q. Wu ◽  
J. C. Chee-Sanford ◽  
S. H. Thomas ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Gene Diversity ◽  
Nitrous Oxide Reductase ◽  
Reductase Gene
Sign in / Sign up

Export Citation Format

Share Document