scholarly journals The Unstable Restorer-of-Fertility Locus in Pepper (Capsicum Annuum. L) is Delimited to a Genomic Region Containing PPR Genes

2021 ◽  
Author(s):  
Moo Chan Kang ◽  
Hwa-Jeong Kang ◽  
So-Young Jung ◽  
Hae-Young Lee ◽  
Min-Young Kang ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Capsicum Annuum ◽  
Fertility Restoration ◽  
Dominant Gene ◽  
Genomic Region ◽  
Gene Isolation ◽  
Hybrid Seed Production ◽  
Capsicum Annuum L ◽  
Restorer Of Fertility ◽  
Ppr Genes

Abstract The use of cytoplasmic-genic male sterility (CGMS) systems greatly increases the efficiency of hybrid seed production. Although marker development and candidate gene isolation have been performed for the Restorer-of-fertility (Rf) gene in pepper (Capsicum annuum L.), the broad use of CGMS systems has been hampered by the instability of fertility restoration among pepper accessions, especially sweet peppers, due to the widespread presence of the Unstable Restorer-of-fertility (Rfu) locus. Therefore, to investigate the genetic factors controlling unstable fertility restoration in sweet peppers, we developed a segregation population from a cross between a male-sterile line and an Rfu-containing line to examine the inheritance of Rfu. Individuals with unstable restoration vs. sterility segregated at a 3:1 ratio, indicating that a single dominant gene controls unstable fertility restoration. Genetic mapping delimited the Rfu locus to a 479 kb genomic region on chromosome 6 flanked by two markers, which is close to but different from the previously identified Rf-containing region. The Rfu-containing region harbors a pentatricopeptide repeat (PPR) gene, along with 13 other candidate genes. In addition, this region is syntenic to the genomic region containing the largest number of Rf-like PPR genes in tomato. Therefore, the dynamic evolution of PPR genes might be responsible for both the restoration and instability of fertility in pepper. During genetic mapping, we developed various molecular markers, including one that co-segregated with Rfu. These markers showed higher accuracy for genotyping than previously developed markers, pointing to their possible use in marker-assisted breeding of sweet peppers.

scholarly journals Molecular mapping of dominant gene responsible for leaf curl virus resistance in chilli pepper (Capsicum annuum L.)

3 Biotech ◽  
2020 ◽  
Vol 10 (4) ◽  
Author(s):  
Hament Thakur ◽  
Salesh Kumar Jindal ◽  
Abhishek Sharma ◽  
Major Singh Dhaliwal
Keyword(s):  
Capsicum Annuum ◽  
Virus Resistance ◽  
Molecular Mapping ◽  
Dominant Gene ◽  
Chilli Pepper ◽  
Capsicum Annuum L ◽  
Leaf Curl Virus ◽  
Leaf Curl

Genetics and distribution of fertility restoration associated RAPD markers in inbreds of pepper (Capsicum annuum L.)

2007 ◽  
Vol 111 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Sanjeet Kumar ◽  
Vineeta Singh ◽  
Major Singh ◽  
Shubha Rai ◽  
Sanjeev Kumar ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Rapd Markers ◽  
Fertility Restoration ◽  
Capsicum Annuum L

scholarly journals The molecular basis for allelic differences suggests Restorer-of-fertility 1 is a complex locus in sugar beet (Beta vulgaris L.)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Takumi Arakawa ◽  
Muneyuki Matsunaga ◽  
Katsunori Matsui ◽  
Kanna Itoh ◽  
Yosuke Kuroda ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Molecular Basis ◽  
Fertility Restoration ◽  
Mitochondrial Protein ◽  
Nuclear Gene ◽  
Pcr Primers ◽  
Hybrid Seed Production ◽  
Dominant Allele ◽  
Restorer Of Fertility ◽  
Complex Locus

Abstract Background Cytoplasmic male sterility (CMS) is a widely used trait for hybrid seed production in many crops. Sugar beet CMS is associated with a unique mitochondrial protein named preSATP6 that forms a 250-kDa complex. Restorer-of-fertility 1 (Rf1) is a nuclear gene that suppresses CMS and is, hence, one of the targets of sugar beet breeding. Rf1 has dominant, semi-dominant and recessive alleles, suggesting that it may be a multi-allelic locus; however, the molecular basis for differences in genetic action is obscure. Molecular cloning of Rf1 revealed a gene (orf20) whose protein products produced in transgenics can bind with preSATP6 to generate a novel 200-kDa complex. The complex is also detected in fertility-restored anthers concomitant with a decrease in the amount of the 250-kDa complex. Molecular diversity of the Rf1 locus involves organizational diversity of a gene cluster composed of orf20-like genes (RF-Oma1s). We examined the possibility that members of the clustered RF-Oma1 in this locus could be associated with fertility restoration. Results Six yet uncharacterized RF-Oma1s from dominant and recessive alleles were examined to determine whether they could generate the 200-kDa complex. Analyses of transgenic calli revealed that three RF-Oma1s from a dominant allele could generate the 200-kDa complex, suggesting that clustered RF-Oma1s in the dominant allele can participate in fertility restoration. None of the three copies from two recessive alleles was 200-kDa generative. The absence of this ability was confirmed by analyzing mitochondrial complexes in anthers of plants having these recessive alleles. Together with our previous data, we designed a set of PCR primers specific to the 200-kDa generative RF-Oma1s. The amount of mRNA measured by this primer set inversely correlated with the amount of the 250-kDa complex in anthers and positively correlated with the strength of the Rf1 alleles. Conclusions Fertility restoration by sugar beet Rf1 can involve multiple RF-Oma1s clustered in the locus, implying that stacking 200-kDa generative copies in the locus strengthens the efficacy, whereas the absence of 200-kDa generative copies in the locus makes the allele recessive irrespective of the copy number. We propose that sugar beet Rf1 is a complex locus.

CMS system and its stimulation in hybrid seed production of Capsicum annuum L.

2017 ◽  
Vol 222 ◽  
pp. 175-179 ◽  
Author(s):  
B.N. Swamy ◽  
N.K. Hedau ◽  
Chaudhari G.V. ◽  
Lakshmi Kant ◽  
A. Pattanayak
Keyword(s):  
Capsicum Annuum ◽  
Seed Production ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Capsicum Annuum L

Mapping and validation of markers linked to Rf gene in sweet pepper (Capsicum annuum var grossum)

2021 ◽  
pp. 443
Author(s):  
Manoj Kumar Nalla ◽  
Vinod Kumar Sharma ◽  
Vinukonda Rakesh Sharma
Keyword(s):  
Capsicum Annuum ◽  
Seed Production ◽  
Sweet Pepper ◽  
Hybrid Seed ◽  
Hybrid Breeding ◽  
Hot Pepper ◽  
Hybrid Seed Production ◽  
Breeding Programs ◽  
Rf Gene ◽  
Restorer Of Fertility

Cytoplasmic male sterility (CMS), is the most valuable system in commercial hybrid seed production in hot pepper. Whereas in sweet pepper (Capsicum annuum var grossum), the unavailability of a stable restorer’s line is one of the major constraints in commercial hybrid breeding. Identifying the markers linked to CMS and nuclear restorer-of-fertility (Rf) genes will help in the production of CMS hybrids in sweet pepper. In the present study, we identified seven markers co-segregating with Rf genes using bulk segregating analysis (BSA) on the F2 Rf-segregating mapping population. Among these, the CRF-SCAR marker proved to be linked to the fertility restorer gene and it could be used for screening of genotypes to identify restorers and non-restorer lines in future Capsicum breeding programs and help in the establishment of CMS system for commercial hybrid seed production.

scholarly journals Identification of Rf9, a Gene Contributing to the Genetic Complexity of Fertility Restoration in Hybrid Wheat

2020 ◽  
Vol 11 ◽  
Author(s):  
Fahimeh Shahinnia ◽  
Manuel Geyer ◽  
Annette Block ◽  
Volker Mohler ◽  
Lorenz Hartl
Keyword(s):  
Fertility Restoration ◽  
Snp Markers ◽  
Genetic Maps ◽  
Hybrid Seed Production ◽  
Hybrid Wheat ◽  
Pentatricopeptide Repeat ◽  
Male Sterile ◽  
Transcription Termination Factor ◽  
Restorer Of Fertility ◽  
Genes Encoding

Wheat (Triticum aestivum L.) is a self-pollinating crop whose hybrids offer the potential to provide a major boost in yield. Male sterility induced by the cytoplasm of Triticum timopheevii is a powerful method for hybrid seed production. Hybrids produced by this method are often partially sterile, and full fertility restoration is crucial for wheat production using hybrid cultivars. To identify the genetic loci controlling fertility restoration in wheat, we produced two cytoplasmic male-sterile (CMS) backcross (BC1) mapping populations. The restorer lines Gerek 79 and 71R1203 were used to pollinate the male-sterile winter wheat line CMS-Sperber. Seed set and numbers of sterile spikelets per spike were evaluated in 340 and 206 individuals of the populations derived from Gerek 79 and 71R1203, respectively. Genetic maps were constructed using 930 and 994 single nucleotide polymorphism (SNP) markers, spanning 2,160 and 2,328 cM over 21 linkage groups in the two populations, respectively. Twelve quantitative trait loci (QTL) controlled fertility restoration in both BC1 populations, including a novel restorer-of-fertility (Rf) locus flanked by the SNP markers IWB72413 and IWB1550 on chromosome 6AS. The locus was mapped as a qualitative trait in the BC1 Gerek 79 population and was designated Rf9. One hundred-nineteen putative candidate genes were predicted within the QTL region on chromosome 6AS. Among them were genes encoding mitochondrial transcription termination factor and pentatricopeptide repeat-containing proteins that are known to be associated with fertility restoration. This finding is a promising step to better understand the functions of genes for improving fertility restoration in hybrid wheat.

Sign in / Sign up

Export Citation Format

Share Document