Molecular Characterization and Transmission Study of the Begomovirus on Hollyhock Plant Causing Leaf Curl Disease for the First Time in Province Punjab, Pakistan

Abstract I. Background: Hollyhock (Alcea rosea) is an ornamental plant belonging to the Malvaceae family and has a remarkable aesthetic and medicinal value. Previously in Pakistan, the hollyhock plant was not found to be infected by begomovirus and the plant first time showed the symptoms of typical leaf curling, puckering as well as thickened veins. II. Methods and Results: During the year 2018, symptomatic samples of the hollyhock plants were collected that exhibited characteristic typical leaf curling, puckering as well as thickened veins. DNA was extracted from the samples and the PCR technique was optimized for the detection of begomovirus followed by sequencing. The samples were detected to be infected with begomovirus by using Av/Ac core, Begomo 01/02, and CLCV 01/02 primer showed positive results with 579bp, 2.8kb, and 1.1kb nucleotide respectively. The betasatellite was amplified by using beta01/02 and CLCuMuBF11/R33 showed positive results with 1400bp and 481bp respectively. Sequencing results showed that diseased hollyhock plants were associated with Cotton leaf curl Multan virus-Rajasthan strain along with Cotton leaf curl Multan betasatellite. III. Conclusion: Hollyhock plants infected by begomovirus has been reported for the first time as a possible source of virus inoculum from Pakistan.

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Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus

Microorganisms ◽

10.3390/microorganisms9020304 ◽

2021 ◽

Vol 9 (2) ◽

pp. 304

Author(s):

Yao Chi ◽

Li-Long Pan ◽

Shu-Sheng Liu ◽

Shahid Mansoor ◽

Xiao-Wei Wang

Keyword(s):

Coat Protein ◽

Molecular Mechanisms ◽

Cotton Leaf ◽

Yeast Two Hybrid ◽

Yeast Two Hybrid System ◽

Vacuolar Protein ◽

Leaf Curl Disease ◽

Two Hybrid ◽

Asia Ii 1 ◽

Leaf Curl

Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the Bemisia tabaci complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of Vta1 in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.

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Engineering broad-spectrum resistance to cotton leaf curl disease by CRISPR-Cas9 based multiplex editing in plants

GM crops & food ◽

10.1080/21645698.2021.1938488 ◽

2021 ◽

pp. 1-12

Author(s):

Muhammad Salman Mubarik ◽

Xiukang Wang ◽

Sultan Habibullah Khan ◽

Aftab Ahmad ◽

Zulqurnain Khan ◽

...

Keyword(s):

Broad Spectrum ◽

Cotton Leaf ◽

Broad Spectrum Resistance ◽

Cotton Leaf Curl Disease ◽

Leaf Curl Disease ◽

Leaf Curl

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Diversity of alphasatellites associated with cotton leaf curl disease in Pakistan

Virology Reports ◽

10.1016/j.virep.2016.05.004 ◽

2016 ◽

Vol 6 ◽

pp. 41-52 ◽

Cited By ~ 3

Author(s):

Komal Siddiqui ◽

Shahid Mansoor ◽

Rob W. Briddon ◽

Imran Amin

Keyword(s):

Cotton Leaf ◽

Cotton Leaf Curl Disease ◽

Leaf Curl Disease ◽

Leaf Curl

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Estimating genetic diversity among selected cotton genotypes and the identificationof DNA markers associated with resistance to cotton leaf curl disease

TURKISH JOURNAL OF BOTANY ◽

10.3906/bot-1505-22 ◽

2015 ◽

Vol 39 ◽

pp. 1033-1041 ◽

Cited By ~ 3

Author(s):

Ammad ABBAS ◽

Muhammad Atif IQBAL ◽

Mehbo-ur RAHMAN ◽

Andrew H. PATERSON

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Cotton Leaf ◽

Cotton Leaf Curl Disease ◽

Cotton Genotypes ◽

Leaf Curl Disease ◽

Leaf Curl

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Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India

Indian Journal of Virology ◽

10.1007/s13337-013-0141-4 ◽

2013 ◽

Vol 24 (2) ◽

pp. 188-198 ◽

Cited By ~ 14

Author(s):

V. Venkataravanappa ◽

C. N. Lakshminarayana Reddy ◽

A. Devaraju ◽

Salil Jalali ◽

M. Krishna Reddy

Keyword(s):

Yellow Vein ◽

Cotton Leaf ◽

Leaf Curl Disease ◽

Leaf Curl

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Maintenance of an Old World Betasatellite by a New World Helper Begomovirus and Possible Rapid Adaptation of the Betasatellite

Journal of Virology ◽

10.1128/jvi.00795-09 ◽

2009 ◽

Vol 83 (18) ◽

pp. 9347-9355 ◽

Cited By ~ 64

Author(s):

Muhammad Shah Nawaz-ul-Rehman ◽

Shahid Mansoor ◽

Rob W. Briddon ◽

Claude M. Fauquet

Keyword(s):

New World ◽

Cotton Leaf ◽

Old World ◽

Tropical Regions ◽

Cabbage Leaf Curl Virus ◽

Chili Leaf Curl Betasatellite ◽

Serious Disease ◽

Leaf Curl Virus ◽

First Time ◽

Leaf Curl

ABSTRACT Begomoviruses (family Geminiviridae) cause major losses to crops throughout the tropical regions of the world. Begomoviruses originating from the New World (NW) and the Old World (OW) are genetically distinct. Whereas the majority of OW begomoviruses have monopartite genomes and whereas most of these associate with a class of symptom-modulating satellites (known as betasatellites), the genomes of NW begomoviruses are exclusively bipartite and do not associate with satellites. Here, we show for the first time that a betasatellite (cotton leaf curl Multan betasatellite [CLCuMuB]) associated with a serious disease of cotton across southern Asia is capable of interacting with a NW begomovirus. In the presence of CLCuMuB, the symptoms of the NW cabbage leaf curl virus (CbLCuV) are enhanced in Nicotiana benthamiana. However, CbLCuV was unable to interact with a second betasatellite, chili leaf curl betasatellite. Although CbLCuV can transreplicate CLCuMuB, satellite accumulation levels in plants were low. However, progeny CLCuMuB isolated after just one round of infection with CbLCuV contained numerous mutations. Reinoculation of one such progeny CLCuMuB with CbLCuV to N. benthamiana yielded infections with significantly higher satellite DNA levels. This suggests that betasatellites can rapidly adapt for efficient transreplication by a new helper begomovirus, including begomoviruses originating from the NW. Although the precise mechanism of transreplication of betasatellites by begomoviruses remains unknown, an analysis of betasatellite mutants suggests that the sequence(s) required for maintenance of CLCuMuB by one of its cognate begomoviruses (cotton leaf curl Rajasthan virus) differs from the sequences required for maintenance by CbLCuV. The significance of these findings and, particularly, the threat that betasatellites pose to agriculture in the NW, are discussed.

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