scholarly journals Combined Topical and Systemic Administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

2020 ◽  
Author(s):  
Yang Zhou ◽  
Bo Zhao ◽  
Liao Xing Zhang ◽  
Jun Yi Lu ◽  
Tao Shou Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
Systemic Administration ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Genes Expression ◽  
Collagen Iii ◽  
Combined Administration

Abstract Background: Cutaneous wound healing and regeneration has become a recognized health challenge in the world, which causes severe damage to the mental and physical health of patients. Human Adipose-derived mesenchymal stem cells (hADSC) play an essential role in wound healing via their paracrine function. Exosomes secreted by hADSC may contribute to this progress. In this study, we investigated the potential clinical application roles of hADSC and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. Methods: hADSC-Exo was isolated from human hADSC by ultracentrifugation. Mice were subjected to a full-thickness skin biopsy experiment and treated with either control vehicle or hADSC or hADSC-Exo by smearing administration (sm) or subcutaneous administration (sc) or intravenous administration (iv). The efficacy of hADSC and hADSC-Exo on wound healing was evaluated by measuring wound closure rates, histological analysis. Results: Combined application of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration offered the additional benefit of promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis. Either topical application of hADSC-Exo or systemic administration with hADSC/hADSC-Exo appeared more effective in stimulating cell proliferation, inhibiting cell apoptosis and inflammation, promoting skin elasticity and barrier integrity, with increased genes expression of PCNA, VEGF, collagen III, Filaggrin, Loricrin, and AQP3, with decreased genes expression of TNF-alpha. Conclusion: Our findings suggest that the combined administration of hADSC/hADSC-Exo can facilitate cutaneous wound healing and reduce scar formation. These data provide the first evidence for the feasibility of smearing of hADSC-Exo as a cell-free therapy in treating cutaneous wounds, and the potential clinical value of combined administration of hADSC/hADSC-Exo.

scholarly journals Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yang Zhou ◽  
Bo Zhao ◽  
Xin-Liao Zhang ◽  
Yi-jun Lu ◽  
Shou-Tao Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Intravenous Administration ◽  
Systemic Administration ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Genes Expression ◽  
Collagen Iii ◽  
Combined Administration

Abstract Background Cutaneous wound healing and regeneration have become a recognized health challenge in the world, which causes severe damage to the mental and physical health of patients. Human adipose-derived mesenchymal stem cells (hADSC) play an essential role in wound healing via their paracrine function. Exosomes secreted by hADSC may contribute to this progress. In this study, we investigated the potential clinical application roles of hADSC and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. Methods hADSC-Exo was isolated from human hADSC by ultracentrifugation. Mice were subjected to a full-thickness skin biopsy experiment and treated with either control vehicle or hADSC or hADSC-Exo by smearing administration (sm) or subcutaneous administration (sc) or intravenous administration (iv). The efficacy of hADSC and hADSC-Exo on wound healing was evaluated by measuring wound closure rates, histological analysis. Results Combined application of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration offered the additional benefit of promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis. Either topical application of hADSC-Exo or systemic administration with hADSC/hADSC-Exo appeared more effective in stimulating cell proliferation, inhibiting cell apoptosis and inflammation, and promoting skin elasticity and barrier integrity, with increased genes expression of PCNA, VEGF, collagen III, Filaggrin, Loricrin, and AQP3, with decreased genes expression of TNF-alpha. Conclusion Our findings suggest that the combined administration of hADSC/hADSC-Exo can facilitate cutaneous wound healing and reduce scar formation. These data provide the first evidence for the feasibility of smearing of hADSC-Exo as a cell-free therapy in treating cutaneous wounds, and the potential clinical value of combined administration of hADSC/hADSC-Exo.

scholarly journals The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism

2019 ◽  
Vol Volume 14 ◽  
pp. 2281-2299 ◽  
Author(s):  
Iwona Lasocka ◽  
Elżbieta Jastrzębska ◽  
Lidia Szulc-Dąbrowska ◽  
Michał Skibniewski ◽  
Iwona Pasternak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cutaneous Wound Healing ◽  
Action Mechanism ◽  
Cutaneous Wound

Delivery of mesenchymal stem cells in a biomimetic collagen hydrogel enhances cutaneous wound healing

2010 ◽  
Vol 211 (3) ◽  
pp. S91-S92 ◽  
Author(s):  
Kristine Rustad ◽  
Victor W. Wong ◽  
Michael Sorkin ◽  
Jason P. Glotzbach ◽  
Dean Nehama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Collagen Hydrogel

Effects of Human Cord Blood Mesenchymal Stem Cells on Cutaneous Wound Healing in Leprdb Mice

2010 ◽  
Vol 65 (6) ◽  
pp. 565-572 ◽  
Author(s):  
Kwan-Chul Tark ◽  
Jong-Won Hong ◽  
Young-Soo Kim ◽  
Seung-Boem Hahn ◽  
Won-Jai Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Cutaneous Wound Healing ◽  
Human Cord Blood ◽  
Cutaneous Wound

scholarly journals Human bone marrow mesenchymal stem cells-derived exosomes stimulate cutaneous wound healing mediates through TGF-β/Smad signaling pathway

2020 ◽  
Author(s):  
Tiechao Jiang ◽  
Zhongyu Wang ◽  
Ji Sun
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Skin Cells ◽  
Smad Signaling Pathway ◽  
Marrow Mesenchymal Stem Cells

Abstract Background: Cutaneous wound healing represents a morphogenetic response to injury, and is designed to restore anatomic and physiological function. Human bone marrow mesenchymal stem cells-derived exosomes (hBM-MSCs-Ex) is a promising source for cell-free therapy and skin regeneration. Methods: In this study, we investigated the cell regeneration effects and its underlying mechanism of hBM-MSCs-Ex on cutaneous wound healing in rats. In vitro studies, we evaluated the role of hBM-MSCs-Ex in the two types of skin cells: human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs) for the proliferation. For in vivo studies, we used a full-thickness skin wound model to evaluate the effects of hBM-MSCs-Ex on cutaneous wound healing in vivo. Results: The results demonstrated that hBM-MSCs-Ex promote both two types of skin cells growth effectively and accelerate the cutaneous wound healing. Interestingly, we found that hBM-MSCs-Ex significantly down-regulated TGF-β1, Smad2, Smad3, and Smad4 expression, while up-regulated TGF-β3 and Smad7 expression in the TGF-β/Smad signaling pathway. Conclusions: Our findings indicated that hBM-MSCs-Ex effectively promote the cutaneous wound healing through inhibiting the TGF-β/Smad signal pathway. The current results providing a new sight for the therapeutic strategy for the treatment of cutaneous wounds.

scholarly journals Human bone marrow mesenchymal stem cells-derived exosomes stimulate cutaneous wound healing mediates through TGF-β/Smad signaling pathway

2020 ◽  
Author(s):  
Tiechao Jiang ◽  
Zhongyu Wang ◽  
Ji Sun
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Skin Cell ◽  
Smad Signaling Pathway ◽  
Marrow Mesenchymal Stem Cells

Abstract Background: Cutaneous wound healing represents a morphogenetic response to injury, and is designed to restore anatomic and physiological function. Human bone marrow mesenchymal stem cells-derived exosomes (hBM-MSCs-Ex) is a promising source for cell-free therapy and skin regeneration. Methods: In this study, we investigated the cell regeneration effects and its underlying mechanism of hBM-MSCs-Ex on cutaneous wound healing in rats. In vitro studies , w e evaluated the role of hBM-MSCs-Ex in the two type s of skin cell s : human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs) for the proliferation . For in vivo studies , we used a full-thickness skin wound model to evaluate the effects of hBM-MSCs-Ex on cutaneous wound healing in vivo . Results: The results demonstrated that hBM-MSCs-Ex promote both two type s of skin cell s growth effectively and accelerate the cutaneous wound healing. Interestingly , we found that hBM-MSCs-Ex significantly down-regulated TGF-β1, Smad2, Smad3, and Smad4 expression, while up-regulated TGF-β3 and Smad7 expression in the TGF-β/Smad signaling pathway . Conclusions: Our findings indicated that hBM-MSCs-Ex effectively promote the cutaneous wound healing through inhibiting the TGF-β/Smad signal pathway . The current result s providing a new sight for the therapeutic strategy for the treatment of cutaneous wounds.

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