IN VITRO PROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SEEDLING EXPLANTS

Guava (Psidium guajava L.) is an exceptional source of vitamin. C. It is also considered to be the most important cultivated species of the Myrtel family. Shoot tip and stem node were taken from seedling germinated in Murashige and Skoog medium (MS) and cultured in the same medium supplemented with 1-3mg/l benzylaminopurine (BA) and 0.1mg/l naphthaleneacetic acid (NAA) or 0.2-2mg/l thidiazuron (TDZ) and 0.1mg/l NAA. Multiple shoots (4-6) were obtained in 4-5 weeks from culture in 1-2mg/l BA and 0.1mg/l NAA, while TDZ caused abnormal shoot growth. Shoots were rooted successfully with 100% frequency in MS medium containing 2mg/l indolebutyric acid and further elongation of shoots was achieved in MS medium, supplemented with lg/l activated charcoal. Regenerated plantlets were successfully established in soil.

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In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

Botanica Orientalis Journal of Plant Science ◽

10.3126/botor.v6i0.2918 ◽

2010 ◽

Vol 6 ◽

pp. 103-105 ◽

Cited By ~ 5

Author(s):

Aditi Singh ◽

Saroj K Sah ◽

Aunji Pradhan ◽

Sabari Rajbahak ◽

Niran Maharajan

Keyword(s):

Medicinal Plant ◽

Callus Induction ◽

Shoot Growth ◽

Tinospora Cordifolia ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Nodal Segment ◽

Root Induction ◽

Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

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REGENERATION OF PLANTLETS FROM HYPOCOTYL DERIVED CALLUS OF MORUS ALBA

Israel Journal of Plant Sciences ◽

10.1080/07929978.1995.10676610 ◽

1995 ◽

Vol 43 (3) ◽

pp. 259-262 ◽

Cited By ~ 4

Author(s):

K. Kathiravan ◽

A. Shajahan ◽

A. Ganapathi

Keyword(s):

Indoleacetic Acid ◽

Adventitious Shoot ◽

Morus Alba ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Indolebutyric Acid ◽

Shoot Buds ◽

Adventitious Shoot Buds ◽

Hypocotyl Segments

Plantlets were regenerated from hypocotyl callus of Morus alba cv. MR2. Calli were established from hypocotyl segments on Murashige and Skoog (MS) medium supplemented with indoleacetic acid (0.5 mg/1) and benzyladenine (BA) (0.5 mg/1). They were transferred to MS medium with different concentrations of naphthaleneacetic acid NAA and BA for four weeks. Adventitious shoot buds were observed by transferring callus onto fresh Linsmaier and Skoog (LS) medium containing NAA (0.5 mg/1) and BA (0.75 mg/1). Shoots produced in vitro were rooted on MS medium with indolebutyric acid (0.75 mg/1).

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Shoot Proliferation, Callus Induction And Plant Regeneration In Tripsacum Laxum Nash

10.21203/rs.3.rs-880060/v1 ◽

2021 ◽

Author(s):

Yuping Xiong ◽

Jinhui Pang ◽

Kunlin Wu ◽

Jaime A. Teixeira Silva ◽

Xinhua Zhang ◽

...

Keyword(s):

Peat Soil ◽

Shoot Proliferation ◽

Multiple Shoots ◽

Dichlorophenoxyacetic Acid ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Axillary Shoots ◽

Rooting Medium ◽

2,4 Dichlorophenoxyacetic Acid

Abstract The peduncles of Tripsacum laxum Nash were used as explants to induce axillary shoots. Multiple shoots were proliferated on Murashige and Skoog (MS) medium to establish, for the first time, efficient shoot proliferation and plant in vitro regeneration systems. Optimal shoot proliferation medium was MS with 3.0 mg/L 6-benzyladenine (BA) and 0.2 mg/L α-naphthaleneacetic acid (NAA), resulting in a shoot proliferation coefficient of 11.0 within 45 d. Optimal rooting medium was MS with 0.1 mg/L NAA and/or 0.1 mg/L indole-3-butyric acid (IBA), inducing 100% root formation from shoots within 30 d. When young roots, leaf sheaths and shoot bases were used as explants, MS medium with 1.0 mg/L thidiazuron (TDZ) and 0.2 mg/L BA induced most shoots, with the least callus. Shoot bases induced beige-white callus and shoots directly on MS medium with 1.0 mg/L TDZ and 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), while leaf sheaths induced beige-white callus and shoots directly on MS medium with 1.0 mg/L TDZ and 0.2 mg/L BA. Rooted plantlets showed 99.3% survival when transplanted into a substrate of vermiculite: peat soil (1:3, v/v).

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Regeneration of Hemidesmus indicus (L.) R. Br. using in vitro nodes: an alternative method for efficient multiplication of shoots

Notulae Scientia Biologicae ◽

10.15835/nsb13210831 ◽

2021 ◽

Vol 13 (2) ◽

pp. 10831

Author(s):

Ashutosh R. PATHAK ◽

Aruna G. JOSHI

Keyword(s):

Multiple Shoots ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Nodal Explant ◽

Mass Propagation ◽

Hemidesmus Indicus ◽

Full Strength ◽

Alternative Method

In vivo nodes of Hemidesmus indicus (L.) R. Br. induced healthy multiple shoots with branching in our earlier studies and thus in the present study, potency of in vitro nodes to regenerate shoots was evaluated. In vitro nodes were excised from eight-week-old shoots and placed in Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and different concentrations of 6-benzyladenine (BA) and kinetin (Kn). After eight weeks, optimum of 5.42 ± 0.36 shoots with 100% response were regenerated in medium supplemented with BA (10 µM) and Kn (5 µM). These healthy shoots were placed in full, half and quarter strengths of liquid MS medium fortified with sucrose (1%) and α-naphthaleneacetic acid (NAA, 1-25 µM) for rooting. Among all the strengths of MS medium, full strength MS medium having 8 µM NAA formed maximum of 3.42 ± 0.55 roots (91.67% response) within four weeks. The protocol is in continuation with earlier study and it was confirmed that a single in vivo nodal explant can regenerate around 385 healthy elongated shoots within 4 months, which will help in mass-propagation of the species.

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PLANT REGENERATION OF AVOCADO (PERSEA AMERICANA MILL) IN VITRO

HortScience ◽

10.21273/hortsci.27.6.696d ◽

1992 ◽

Vol 27 (6) ◽

pp. 696d-696

Author(s):

Yaseen Mohamed-Yaseen ◽

Raymond J. Schnell ◽

Robert J. Knight ◽

T.L. Davenport

Keyword(s):

Tissue Culture ◽

Multiple Shoots ◽

Persea Americana ◽

Naphthalene Acetic Acid ◽

Root Induction ◽

Ms Medium ◽

Indolebutyric Acid ◽

Embryonic Axes ◽

Phenolic Oxidation

A procedure was developed to regenerate plants via tissue culture from embryonic axes of mature avocado seeds. Explants were cultured in Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and naphthalene-acetic acid (NAA) or thidiazuron (TDZ) and NAA. Culture were kept in the dark for 7-10 days to reduce browning resulting from phenolic oxidation. Multiple shoots (5-8) were formed after transfer to light. Further multiplication were achieved using different combination of BA and NAA or TDZ and NAA. Shoots were cultured in MS supplemented with 2mg/l indolebutyric acid (IBA) for 2 weeks then transferred to MS supplemented with lg/l activated charcoal for root induction. Complete plants were obtained in vitro.

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A Novel Method for Rapid Micropropagation of Pineapple

HortScience ◽

10.21273/hortsci.30.1.127 ◽

1995 ◽

Vol 30 (1) ◽

pp. 127-129 ◽

Cited By ~ 23

Author(s):

E. Kiss ◽

J. Kiss ◽

G. Gyulai ◽

L.E. Heszky

Keyword(s):

Growth Regulator ◽

Shoot Elongation ◽

Ananas Comosus ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Regeneration Rate ◽

Novel Method ◽

N6 Medium ◽

Regenerated Plantlets

A novel micropropagation method for pineapple (Ananas comosus L.), based on shoot elongation induced in vitro, was demonstrated for two cultivars. Decapitated in vitro plantlets were used as explants. Shoot etiolation was induced by placing explants in a Murashige and Skoog (MS) medium containing NAA (10 μm) and incubating in darkness at 28C for 30 to 40 days. The mean number of the regenerated etiolated shoots per explant was 2.6 ± 0.29. The etiolated shoots were placed into N6 medium supplemented with kinetin or BA (25 or 20 μm, respectively). After 4 to 6 weeks, shoots regenerated along the nodes. The highest regeneration rate was 15 and 13 plantlets per node with 25 μm kinetin and 20 mm BA, respectively. Regenerated plantlets were rooted on a growth-regulator-free MS medium. Residual shoots of the initial explants could be recycled by rooting on a growth-regulator-free MS medium. This procedure enables the regeneration of several thousand plantlets per year. Chemical names used: naphthaleneacetic acid (NAA); benzyladenine (BA).

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Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant

Notulae Scientia Biologicae ◽

10.15835/nsb417187 ◽

2012 ◽

Vol 4 (1) ◽

pp. 86-93 ◽

Cited By ~ 6

Author(s):

Mohammad Serajur RAHMAN ◽

Mohammad Abdul Bari MIAH ◽

Mohammad Shahadat HOSSAIN ◽

Ahmad Humayan KABIR ◽

Mohammad Motiur RAHMAN

Keyword(s):

In Vitro Regeneration ◽

Shoot Formation ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Ms Medium ◽

Liquid Media ◽

Indolebutyric Acid ◽

Abrus Precatorius ◽

Isolated Cell

A new protocol has been developed for cell culture and in vitro regeneration of Abrus precatorius that holds enormous potentiality for preparation of medicines. In vitro grown calli were cultured in Murashige and Skoog (MS) liquid media in agitated condition fortified with 0.5 mg/l 6-Benzylaminopurine. Growth curve of cells revealed that the cells continued to grow until 12 days of culture and got the highest peak from day 6-8. Isolated cell was found to produce highest 8.2% calli when suspended on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. Callus derived from single cell produced highest number of embryo (25-28%) cultured on MS medium fortified with 2.0 mg/l 6-Benzylaminopurine and 0.2 mg/l 1-Naphthaleneacetic acid. The bipolar embryos were selected and optimum shoot formation was recorded on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. The optimum root induction was noticed in MS medium supplemented with 1.0 mg/l 3-Indolebutyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

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Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

Jahangirnagar University Journal of Biological Sciences ◽

10.3329/jujbs.v4i1.27786 ◽

2016 ◽

Vol 4 (1) ◽

pp. 61-69 ◽

Cited By ~ 1

Author(s):

S Yesmin ◽

A Hashem ◽

MS Islam

Keyword(s):

Morphological Variation ◽

Multiple Shoots ◽

Nodal Segments ◽

Induction Medium ◽

Root Induction ◽

Ms Medium ◽

Eclipta Alba ◽

Half Strength ◽

Regenerated Plantlets

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

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Clonal propagation of guava (Psidium guajava L) on nodal explants of mature elite cultivar

International Journal of Plant Biology ◽

10.4081/pb.2011.e2 ◽

2011 ◽

Vol 2 (1) ◽

pp. 2 ◽

Cited By ~ 6

Author(s):

Xiaomei Liu ◽

Guochen Yang

Keyword(s):

Microbial Contamination ◽

Culture Medium ◽

Clonal Propagation ◽

Psidium Guajava ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Micro Propagation ◽

Elite Cultivar ◽

Subtropical Fruit

Guava (Psidium guajava L.) is a very valuable tropical and subtropical fruit. However, guava micro-propagation are genotypes dependent, there are several problems associated with in vitro cultures of guava including browning or blackening of culture medium due to leaching of phenolics, microbial contamination, and in vitro tissue recalcitrance. A micro-propagation system using Murashige and Skoog (MS) medium with 6-benzylaminopurine (BA), kinetin and naphthaleneacetic acid (NAA) was developed for guava (Psidium guajava L) from mature cultivar. As part of this research various disinfection methods and plant growth regulators were tested in vitro. The most effective method involved treating explants in a 15% bleach solution for 20 mins followed by culturing them in MS medium with 250 mg/L polyvinylpyrrolidone (PVP). This method maximized the percentage of bud breakage (53.3%), while producing the minimum browning rate (18.3%) for the explants. The best observed proliferation rate (71.2%) occurred on the MS medium supplemented with 4.44 μM BA, 4.65 μM kinetin (KT) and 0.54 μM NAA. It produced the highest mean number of shoots (2.2). Shoots were then rooted (65%) when dipped in 4.9 mM Indole-3-butyric acid (IBA) solution for 1 min and rooted plantlets survived (100%) after acclimatization to the greenhouse.

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The Effects of Growth Regulators on Shoot Propagation and Rooting of Vitis Following in Vitro Axillary Bud and Shoot Apex Culture

HortScience ◽

10.21273/hortsci.32.3.516c ◽

1997 ◽

Vol 32 (3) ◽

pp. 516C-516

Author(s):

Handan Büyükdemirci ◽

Paul E. Read

Keyword(s):

Shoot Apex ◽

Shoot Growth ◽

Shoot Proliferation ◽

Multiple Shoots ◽

Multiple Shoot ◽

Axillary Buds ◽

Axillary Bud ◽

Ms Medium ◽

Axillary Bud Culture

Axillary buds of `Valiant' grapevine (Vitis spp.) grown in vitro were transferred onto Murashige and Skoog (MS) medium supplemented with different cytokinin and auxin combinations and concentrations. It was found that culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots, number of roots, and length of shoots. MS medium supplemented with 1.0 mg BA/liter in combination with 0.01 mg NAA/L was found to be the best medium for shoot growth and callus production. MS medium supplemented with the combination of 0.5 mg BA/L and 0.01 mg NAA/L was the best medium for explant rooting. The medium containing BA and NAA encouraged better shoot growth than those containing BA alone. When the concentration of BA in the medium was increased, multiple shoot proliferation and teratological structures of explants increased, but the number of small leaves and length of internode decreased. Axillary bud culture led to better shoot growth than was found for shoot apex culture. The presence of leaves positively affected shoot growth from axillary buds. Also placing the axillary buds horizontally onto the medium gave better shoot proliferation and growth than placing them vertically.

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