scholarly journals Screening and Characterization of 11 Novel Microsatellite Markers from Viburnum dilatatum

HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1456-1459 ◽  
Author(s):  
Deborah Dean ◽  
Phillip A. Wadl ◽  
Xinwang Wang ◽  
William E. Klingeman ◽  
Bonnie H. Ownley ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Cultivar Development ◽  
Expected Heterozygosity ◽  
Horticultural Traits ◽  
Wide Range ◽  
Relationship Of ◽  
Ornamental Shrub

Viburnum dilatatum is a popular and economically important ornamental shrub. The wide range of desirable horticultural traits, paired with a propensity for seedlings to become invasive, has created interest in the genetics and breeding of this species. To investigate the genetic diversity of V. dilatatum, microsatellite loci were identified from a GT-enriched genomic library constructed from V. dilatatum ‘Asian Beauty’. Eleven microsatellite loci have been characterized on a group of 16 different related V. dilatatum cultivars and hybrids. Two to 12 alleles were identified per locus, and the polymorphism information content (PIC) values ranged from 0.36 to 0.87. Expected heterozygosity (He) ranged from 0.48 to 0.88 and observed heterozygosity (Ho) ranged from 0 to 0.73. This set of molecular markers also exhibited expected transferability between various V. dilatatum cultivars and two hybrids with V. japonicum. As a consequence, these markers will aid in breeding for new cultivar development, assist with early detection and screening of plants that have escaped cultivation, and are expected to help in refining the phylogenetic relationship of V. dilatatum to other species and genera within the Adoxaceae.

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

Development and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnh

2009 ◽  
Vol 1 (1) ◽  
pp. 465-469 ◽  
Author(s):  
Janete Motta da Silva ◽  
Adna Cristina Barbosa de Sousa ◽  
Anete Pereira de Souza ◽  
Edson Seizo Mori ◽  
Miguel Luiz Menezes Freitas ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Genomic Library ◽  
Eucalyptus Camaldulensis ◽  
Enriched Genomic Library ◽  
Eucalyptus Camaldulensis Dehnh

scholarly journals Development and characterization of twelve new polymorphic microsatellite loci in the Iberian ribbed newt, Pleurodeles waltl (Caudata: Salamandridae), with data on cross-amplification in P. nebulosus

2014 ◽  
Vol 35 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Jorge Gutiérrez-Rodríguez ◽  
Elena G. Gonzalez ◽  
Íñigo Martínez-Solano
Keyword(s):  
Population Dynamics ◽  
Microsatellite Loci ◽  
Evolutionary History ◽  
The Novel ◽  
Expected Heterozygosity ◽  
History Of ◽  
Southern Iberia ◽  
Polymorphic Microsatellite ◽  
Pleurodeles Waltl

Twelve novel polymorphic microsatellite loci were isolated and characterized for the Iberian ribbed newt, Pleurodeles waltl (Caudata, Salamandridae). The distribution of this newt ranges from central and southern Iberia to northwestern Morocco. Polymorphism of these novel loci was tested in 40 individuals from two Iberian populations and compared with previously published markers. The number of alleles per locus ranged from two to eight. Observed and expected heterozygosity ranged from 0.13 to 0.57 and from 0.21 to 0.64, respectively. Cross-species amplification was tested in Pleurodeles nebulosus, which is listed as Vulnerable by the IUCN. Eight new and seven previously published loci amplified successfully in that species and thus represent a valuable conservation tool. The novel microsatellites will be useful for a better understanding of the population dynamics, demography, genetic structure, and evolutionary history of Pleurodeles waltl and P. nebulosus.

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