scholarly journals In Vitro Regeneration from Longitudinal Sections of Seedlings of Beaucarnea purpusii Rose, an Endemic and Endangered Species

HortScience ◽  
2016 ◽  
Vol 51 (3) ◽  
pp. 279-284 ◽  
Author(s):  
María del Carmen Vadillo-Pro ◽  
Luis Hernández-Sandoval ◽  
Guadalupe Malda-Barrera ◽  
María Luisa Osorio-Rosales ◽  
Martín Mata-Rosas
Keyword(s):  
Liquid Culture ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Sustainable Use ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Adventitious Shoot Formation ◽  
Efficient Alternative

The present study establishes an efficient protocol for in vitro propagation from longitudinal sections of seedlings of Beaucarnea purpusii, a threatened and highly appreciated ornamental species. The effect of three cytokinins: N6-benzyladenine (BA), kinetin (Kin), and thidiazuron, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ), in semisolid media and three different concentrations, as well as the effect of BA and TDZ pulses at higher concentrations in liquid culture medium, were investigated. Adventitious shoot formation by direct organogenesis was observed from all treatments. Additionally, adventitious shoot formation was recorded from the leaves of the new shoots; this particular response was exclusive to treatments supplemented with TDZ. In the experiment using semisolid culture media, the highest means of shoots per explant were obtained from treatments containing TDZ, particularly at a concentration of 0.45 μm (25.8 shoots per explant). For the pulses experiment, the liquid culture media supplemented with TDZ at 22.35 µm for 24 hours and 136.21 µm for 96 hours, induced a mean of 3.9 and 3.3 shoots per explant, respectively. Subculturing individual shoots on MS and half-strength MS (1/2MS) media, both supplemented with activated charcoal at 1 g·L−1, induced rooting in 85% to 95% of shoots. A survival rate of 100% under greenhouse conditions was achieved. The results of this study provide an efficient alternative for mass propagation of B. purpusii and may also contribute to the conservation and sustainable use of this valuable natural resource.

scholarly journals In Vitro Regeneration through Direct Organogenesis from Protocorms of Oncidium tigrinum Llave & Lex. (Orchidaceae), an Endemic and Threatened Mexican Species

HortScience ◽  
2011 ◽  
Vol 46 (8) ◽  
pp. 1132-1135 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Rosario Julieta Baltazar-García ◽  
Victor Manuel Chávez-Avila
Keyword(s):  
Culture Media ◽  
In Vitro Regeneration ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation

A protocol for in vitro propagation from protocorms of Oncidium tigrinum Llave & Lex., a threatened species distributed in Mexico and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6-benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg·L−1) and/or α-naphthaleneacetic acid (NAA) at 0, 0.1, and 0.5 mg·L−1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm-like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg·L−1 in combination with at NAA 0.1 mg·L−1. The average height of shoots was three times greater in MS medium than in KCm medium. Subculturing individual shoots in MS medium without plant growth regulators, but with 1 g·L−1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

scholarly journals In vitro studies on Dendrobium fimbriatum Hk.F – An endangered orchid

2019 ◽  
pp. 100-103
Author(s):  
T. Ramesh ◽  
P. Renganathan ◽  
M. Prabhakaran
Keyword(s):  
Culture Media ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Dendrobium Fimbriatum

A protocol for in vitro propagation from protocorms of Dendrobium fimbriatum a distributed in all over world and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6- benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg_L–1) and/or a-naphthalene acetic acid (NAA) at 0, 0.1, and 0.5 mg_L–1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg_L–1 in combination with at NAA 0.1 mg_L–1. The average height of shoots was three times greater in MS medium than in KC m medium. Sub culturing individual shoots in MS medium without plant growth regulators, but with 1 g_L–1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

scholarly journals Developmental Anatomy of Adventitious Shoot Formation on Antirrhinum majus L. Hypocotyls In Vitro

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 813C-813
Author(s):  
James S. Busse ◽  
Monica Figueroa-Cabanas ◽  
Dennis P. Stimart*
Keyword(s):  
Cell Division ◽  
Vascular System ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Epidermal Cells ◽  
Antirrhinum Majus ◽  
Direct Organogenesis ◽  
Developmental Anatomy ◽  
Adventitious Shoot Formation

Adventitious shoot formation in vitro from Antirrhinum majus L. hypocotyls was investigated using two inbred lines, the most and least regenerative lines selected from screening. Time course analysis indicated cell division in the most regenerative line occurred first in one or a small number of epidermal cells with periclinal and anticlinal divisions. Subsequently, cortical then vascular cells were recruited beneath the dividing epidermal cells. Once shoots formed, their vascular system was continuous with the original hypocotyl explant. The least regenerative line had no cell division directed toward organogenesis. Shoot formation on hypocotyls of A. majus was adventitious in origin, by direct organogenesis and genotype dependent.

scholarly journals Developmental Anatomy of Adventitious Shoot Formation on Snapdragon (Antirrhinum majus L.) Hypocotyls In Vitro

2005 ◽  
Vol 130 (2) ◽  
pp. 147-151 ◽  
Author(s):  
James S. Busse ◽  
M. Figueroa-Cabanas ◽  
D.P. Stimart
Keyword(s):  
Cell Division ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Epidermal Cells ◽  
Antirrhinum Majus ◽  
Direct Organogenesis ◽  
Induction Medium ◽  
Developmental Anatomy ◽  
Adventitious Shoot Formation

Developmental anatomy of adventitious shoot formation in vitro from snapdragon (Antirrhinum majus L.) hypocotyls was investigated using two inbred lines, the most (R) and least (NR) regenerative lines selected from screening (Schroeder and Stimart, 1999). Time course analysis indicated cell division in the most regenerative line occurred first in one or a small number of epidermal cells with periclinal and anticlinal divisions within 2 days of placing hypocotyls on induction medium. Subsequently, cortical then vascular cells were recruited beneath the dividing epidermal cells. Once shoots formed, their vascular system was continuous with the original hypocotyl explant. The least regenerative line had no cell division directed towards organogenesis through 6 days. Shoot formation on snapdragon hypocotyls was adventitious in origin, by direct organogenesis and genotype dependent.

In vitro studies of adventitious shoot formation in Pinus contorta

1981 ◽  
Vol 59 (5) ◽  
pp. 870-874 ◽  
Author(s):  
Sara Von Arnold ◽  
Tage Eriksson
Keyword(s):  
Pinus Contorta ◽  
Stem Elongation ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Buds ◽  
Adventitious Shoot Formation ◽  
Bud Induction ◽  
Further Development

Isolated embryos of Pinus contorta Dougl. ex Loud, were induced to form adventitious buds on a cytokinin-supplemented medium. Further development of the buds required transfer to a cytokininless medium. Both bud induction and development were stimulated by a dilution of the basal culture medium and best growth was obtained if the buds were isolated from the original tissue when stem elongation had started. The growth of these isolated adventitious shoots was further stimulated by adding activated charcoal to the diluted medium. A small percentage of the shoots have been rooted. The capacity for bud formation varied among seeds collected from different regions of British Columbia. This method for induction of adventitious buds on embryos was also applicable to explants of young seedlings.

scholarly journals 882 PB 276 IN VITRO PROPAGATION AND CHIMERAL TRAITS OF Cryptanthus `Marian Oppenheimer' (WIDE LEAF CLONE)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560c-560
Author(s):  
Yong Cheong Koh ◽  
Fred T. Davies
Keyword(s):  
In Vitro Propagation ◽  
Callus Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Periclinal Chimera ◽  
Adventitious Shoot Formation ◽  
L1 And L2

The leaves of vegetative stolons of greenhouse grown Cryptanthus `Marian Oppenheimer' (wide leaf clone) were cultured in modified MS media to induce adventitious shoot formation via callus formation. The best callus induction medium was basal MS medium with 10 μM NAA, IBA and BA. Pure green (843), maroon (3), striped (2) and albino plantlets were obtained. Most of the albino plantlets were stunted, tightly clumped together and impossible to score. The medium which produced the highest average number of non-albino plantlets was basal MS medium with 0.3 μM NAA, IBA and BA All non-albino plantlets were rooted in MS medium with 5.4 μM NAA and transplanted ex vitro with a survival rate of 96.7%. The maroon plantlets became green two weeks after transplanting. Histological studies revealed that C. `Marian Oppenheimer' (wide leaf clone) has two tunicas (L1 and L2) and a corpus (L3). Callus on the leaf explant arose mainly from the L2 and L3. Apparently C. `Marian Oppenheimer' (wide leaf clone) is a GWG periclinal chimera.

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