scholarly journals Thidiazuron- and Zeatin-induced High-frequency Shoot Regeneration from Leaf and Shoot-tip Explants of Strawberry

2013 ◽  
Vol 23 (3) ◽  
pp. 276-281 ◽  
Author(s):  
Fatemeh Haddadi ◽  
Maheran Abd Aziz ◽  
Hossein Kamaladini ◽  
Seyed Ali Ravanfar
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Leaf Explant ◽  
Direct Organogenesis ◽  
Regeneration Response ◽  
Basal Salts ◽  
Shoot Tip Explants

Strawberry (Fragaria ×ananassa cv. Camarosa) was evaluated to determine a high-frequency shoot regeneration response for leaf and shoot-tip explants. For direct organogenesis from strawberry leaves, combinations of moderate concentrations of thidiazuron [TDZ (0, 2, and 4 μm)] and 6-benzylaminopurine [BAP (0, 4, and 9 μm)] added into medium containing Murashige and Skoog (MS) basal salts were compared. The most shoots regenerated per leaf explant were observed with 4-μm TDZ. Regeneration from shoot tips was evaluated with 0-, 2-, 4-, 8-, and 16-μm zeatin, kinetin, or 6-α,α-dimethylallylamino purine (2ip) tested individually. Optimum shoot proliferation was achieved from shoot-tip explants on medium containing 4-μm zeatin. Rooting was best without cytokinins in the medium; however, adequate rooting was obtained on the 4-μm zeatin treatment as well.

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

scholarly journals In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

2017 ◽  
Vol 2 ◽  
pp. 113-119 ◽  
Author(s):  
Marziyeh Jafari ◽  
Mohammad Hosein Daneshvar ◽  
Amin Lotfi
Keyword(s):  
Shoot Proliferation ◽  
Cotyledonary Node ◽  
Shoot Tip ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

scholarly journals Micropropagation of Members of the Cactaceae Subtribe Cactinae

1990 ◽  
Vol 115 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Philip W. Clayton ◽  
John F. Hubstenberger ◽  
Gregory C. Phillips ◽  
S. Ann Butler-Nance
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tip ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Production ◽  
Series Of Experiments ◽  
Basal Media ◽  
Free Media ◽  
Shoot Tip Explants

Micropropagation of 11 rare or endangered cacti species belonging to the subtribe Cactinae was achieved by rooting of proliferated axillary shoots. Shoot tip explants were obtained from seedlings of Escobaria missouriensis D.R. Hunt, E. robbinsorum (Earle) D.R. Hunt, Sclerocactus spinosior (Engelm.) Woodruff & L. Benson, and Toumeya papyracantha (Engelm.) Br. & Rose, and from mature plants of Mammillaria wrightii Engelm., Pediocactus bradyi L. Benson, P. despainii Welsh & Goodrich, P. knowltonii L. Benson, P. paradinei B.W. Benson, P. winkleri Heil, and S. mesae-verdae (Boissevain) L. Benson. Three or four species were used in each of a series of experiments investigating the effects of basal media and auxin and cytokinin types and concentrations on axillary shoot proliferation. Low or no auxin but moderate to high cytokinin concentrations were required for axillary shoot production. All species rooted spontaneously on hormone-free media; however, several species rooted better on media containing auxin. All species were re-established in the greenhouse.

scholarly journals Studies on High Frequency Shoot Regeneration in Sesame (Sesamum indicum L.)

2012 ◽  
Vol 21 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Bangaramma S. Wadeyar ◽  
R. Lokesha
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
High Frequency ◽  
Sesamum Indicum ◽  
Direct Seeding ◽  
The Other ◽  
Seeding Method ◽  
Regeneration Response ◽  
High Sucrose

High frequency shoot regeneration was attempted in sesame (Sesamum indicum L. Pedaliaceae), using five genotypes/varieties viz. Tumkur and Gulbarga Locals (land races), W-II, E-8 and DS-1 (varieties). The hypocotyl-derived callus obtained through direct seeding method was placed on MS with five different treatments viz. pre-culture of callus on high sucrose (6 - 9%) for two weeks and transferring on to plain MS with 3% sucrose, MS with variable concentrations of TDZ alone, MS with constant TDZ and variable concentrations of BA and IAA, MS with variable concentrations of BA and IAA without TDZ, MS with ABA and AgNO3 and MS with BAP, NAA and AgNO3.  Highest frequency of cent per cent shoot regeneration was initiated in variety DS-1on MS containing NAA 2.5 mg/l, BAP 3.5 mg/l and 20 µM AgNO3 with 2.50 mean shoots/callus followed by 91.6% in variety W-II on MS containing 25 µM TDZ with 2.20 mean shoots/callus. The other treatments had poor shoot regeneration response. The results have been discussed in the light of sesame improvement through biotechnological ways.   Key words: Sesamum indicum, hypocotyl, high frequency,  shoot regeneration   D. O. I. 10.3329/ptcb.v21i1.9562   Plant Tissue Cult. & Biotech. 21(1): 45-52, 2011 (June)

scholarly journals 252 STUDIES ON ADVENTITIOUS REGENERATION IN COCOYAM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 465f-465
Author(s):  
Leopold M. Nyochembeng ◽  
Stephen Garton
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Shoot Tips ◽  
Adventitious Regeneration ◽  
Petiole Explants ◽  
The Impact

Studies were conducted to determine the response of cocoyam shoot tips, petioles, cotyledons and hypocotyls in various media for callus formation, adventitious shoot development and somatic embryogenesis. In all experiments, B5 basal medium or low N B5 were supplemented with various growth regulators. High frequency adventitious shoot proliferation was obtained using cotyledons and hypocotyls in medium supplemented with 1 mg/l IBA and 0.5 mg/l TDZ. Embryogenic callus was obtained using shoot tips in media containing 1 mg/l Dicamba, hile somatic embryos were observed in media containing 0.3 mg/l 2, 4 - D and 1 mg/l Kinetin, using hypocotyl and petiole explants. The impact of these results on micropropagation of cocoyam is discussed.

scholarly journals Callus Induction and Plantlet Regeneration in Blackgram (Vigna mungo L. Hepper)

2013 ◽  
Vol 19 (2) ◽  
pp. 27-35 ◽  
Author(s):  
SA Mony ◽  
MS Haque ◽  
MA Karim ◽  
SK Roy
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Plantlet Regeneration ◽  
Shoot Tip ◽  
Root Tip ◽  
Ms Medium ◽  
Plantlet Formation ◽  
Rooting Medium ◽  
Shoot Tip Explants

The present study was undertaken for callus induction and subsequent plantlet regeneration in blackgram. The study comprised of experiments for callus initiation, shoot regeneration and plantlet formation from cotyledon, hypocotyl, root tip and shoot tip explants. The effects of explants and different concentrations and combinations of BAP (0.0, 1.0, 2.5, 5.0 and 10 mg L-1) and NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg L-1)) on callus induction were investigated first. Among the explants, hypocotyls showed the best performance in callus formation (92.33%) when cultured on MS medium supplemented with 2.5 mg L-1) BAP and 1.5 mg L-1) NAA followed by cotyledon, shoot tip and root tip explants, respectively. The height percentage of shoot regeneration from the calli derived from hypocotyls (56.33%) was achieved in MS medium supplemented with 3.0 mg L-1) BAP and 0.3 mg L-1) NAA and 0.5 mg L-1) GA3. Calli from other explants had no shoot regeneration. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100 %) of rooting was observed with MS medium supplemented with 0.5 mg L-1) IBA. The rooted plants were transferred to pots for hardening.DOI: http://dx.doi.org/10.3329/pa.v19i2.16917 Progress. Agric. 19(2): 27 - 35, 2008

High frequency in vitro shoot regeneration of Capparis deciduas from shoot tip culture

2007 ◽  
Vol 09 (02) ◽  
pp. 153-156
Author(s):  
S.N. Saxena ◽  
S. Janjharia ◽  
A.K. Sharma ◽  
R. Sharma ◽  
G. Singh
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Shoot Tip ◽  
Shoot Tip Culture ◽  
In Vitro Shoot Regeneration

scholarly journals In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

2009 ◽  
Vol 18 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H. David Raja ◽  
D.I. Arockiasamy
Keyword(s):  
High Frequency ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Root Induction ◽  
Root Proliferation ◽  
Key Words Micropropagation ◽  
Shoot Tip Explants ◽  
Important Medicinal Plant

A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 - 4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 - 2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 - 95% after 25 days. Key words: Micropropagation, Mentha viridis, Node, Shoot tip, Multiple shoots D.O.I. 10.3329/ptcb.v18i1.3243 Plant Tissue Cult. & Biotech. 18(1): 1-6, 2008 (June)

Sign in / Sign up

Export Citation Format

Share Document