scholarly journals Plum Pox Virus Coat Protein Gene Intron-hairpin-RNA (ihpRNA) Constructs Provide Resistance to Plum Pox Virus in Nicotiana benthamiana and Prunus domestica

2007 ◽  
Vol 132 (6) ◽  
pp. 850-858 ◽  
Author(s):  
Jean-Michel Hily ◽  
Michel Ravelonandro ◽  
Vern Damsteegt ◽  
Carole Bassett ◽  
Cesar Petri ◽  
...  
Keyword(s):  
Coat Protein ◽  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
High Capacity ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Hairpin Rna ◽  
Woody Perennial ◽  
Virus Coat ◽  
Interfering Rna

Constructs with self-complementary sequences separated by an intron produce “hairpin” RNA [intron-hairpin-RNA (ihpRNA)] structures that efficiently elicit posttranscriptional gene silencing (PTGS). In the current study, the authors use this technology to confer resistance to plum pox virus (PPV) in herbaceous and woody perennial plants by silencing the PPV–coat protein (CP) gene. The authors confirmed the high capacity of ihpRNA constructs for inducing RNA silencing in Nicotiana benthamiana Domin., as more than 75% of the transformants displayed PTGS as evaluated by specific small interfering RNA (siRNA) production. The authors demonstrated that ihpRNA constructs provided PPV resistance, and they found a correlation between the length of the PPV sequence introduced in the ihpRNA constructs and the frequency of transgenic-resistant plants. Plants transformed with the full-length sequence produced a higher percentage of resistant lines. The authors further demonstrated for the first time that ihpRNA technology is applicable to a woody perennial species. A transgenic plum (Prunus domestica L.) PPV-CP ihpRNA line showed gene silencing characteristics (hypermethylation of the transgene sequence and specific siRNA production) and resistance to PPV infection 16 months after inoculation.

Transgenic plums (Prunus domestica L.) express the plum pox virus coat protein gene

1994 ◽  
Vol 14 (1) ◽  
Author(s):  
Ralph Scorza ◽  
Michel Ravelonandro ◽  
AnnM. Callahan ◽  
JohnM. Cordts ◽  
Marc Fuchs ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions

2004 ◽  
Vol 13 (5) ◽  
pp. 427-436 ◽  
Author(s):  
Jean-Michel Hily ◽  
Ralph Scorza ◽  
Tadeusz Malinowski ◽  
Barbara Zawadzka ◽  
Michel Ravelonandro
Keyword(s):  
Gene Silencing ◽  
Field Conditions ◽  
Plum Pox Virus ◽  
Prunus Domestica

scholarly journals Effect of Grafting on Viral Resistance of Non-transgenic Plum Scion Combined With Transgenic PPV-Resistant Rootstock

2021 ◽  
Vol 12 ◽  
Author(s):  
Tatiana Sidorova ◽  
Dmitry Miroshnichenko ◽  
Ilya Kirov ◽  
Alexander Pushin ◽  
Sergey Dolgov
Keyword(s):  
Sequence Data ◽  
Fruit Trees ◽  
Viral Resistance ◽  
Plum Pox Virus ◽  
Hairpin Rna ◽  
Rnai Silencing ◽  
Resistant Rootstock ◽  
Foreign Genes ◽  
Specific Degradation ◽  
Interfering Rna

In stone fruit trees, resistance to Plum pox virus (PPV) can be achieved through the specific degradation of viral RNA by the mechanism of RNA interference (RNAi). Transgenic virus-resistant plants, however, raise serious biosafety concerns due to the insertion and expression of hairpin constructs that usually contain various selective foreign genes. Since a mature stone tree represents a combination of scion and rootstock, grafting commercial varieties onto transgenic virus-tolerant rootstocks is a possible approach to mitigate biosafety problems. The present study was aimed at answering the following question: To what extent are molecular RNAi silencing signals transmitted across graft junctions in transgrafted plum trees and how much does it affect PPV resistance in genetically modified (GM)/non-transgenic (NT) counterparts? Two combinations, NT:GM and GM:NT (scion:rootstock), were studied, with an emphasis on the first transgrafting scenario. Viral inoculation was carried out on either the scion or the rootstock. The interspecific rootstock “Elita” [(Prunus pumila L. × P. salicina Lindl.) × (P. cerasifera Ehrh.)] was combined with cv. “Startovaya” (Prunus domestica L.) as a scion. Transgenic plum lines of both cultivars were transformed with a PPV-coat protein (CP)-derived intron-separate hairpin-RNA construct and displayed substantial viral resistance. High-throughput sequence data of small RNA (sRNA) pools indicated that the accumulation of construct-specific small interfering RNA (siRNA) in transgenic plum rootstock reached over 2%. The elevated siRNA level enabled the resistance to PPV and blocked the movement of the virus through the GM tissues into the NT partner when the transgenic tissues were inoculated. At the same time, the mobile siRNA signal was not moved from the GM rootstock to the target NT tissue to a level sufficient to trigger silencing of PPV transcripts and provide reliable viral resistance. The lack of mobility of transgene-derived siRNA molecules was accompanied by the transfer of various endogenous rootstock-specific sRNAs into the NT scion, indicating the exceptional transitivity failure of the studied RNAi signal. The results presented here indicate that transgrafting in woody fruit trees remains an unpredictable practice and needs further in-depth examination to deliver molecular silencing signals.

scholarly journals Co-expressing Turnip Crinkle Virus-coat protein with the serine protease α-thrombin precursor (pFIIa) in Nicotiana benthamiana Domin

2019 ◽  
Vol 55 (1) ◽  
pp. 88-98 ◽  
Author(s):  
Melina Laguia-Becher ◽  
Zurima Zaldúa ◽  
Weijie Xu ◽  
Patricia Laura Marconi ◽  
William Velander ◽  
...  
Keyword(s):  
Coat Protein ◽  
Serine Protease ◽  
Nicotiana Benthamiana ◽  
Turnip Crinkle Virus ◽  
Virus Coat Protein ◽  
Virus Coat

THE DEVELOPMENT OF GENETIC RESISTANCE TO PLUM POX VIRUS IN TRANSGENIC NICOTIANA BENTHAMIANA AND PRUNUS DOMESTICA

2009 ◽  
pp. 665-672 ◽  
Author(s):  
A. Wang ◽  
L. Tian ◽  
T.-S. Huang ◽  
D.C.W. Brown ◽  
A.M. Svircev ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Genetic Resistance ◽  
Plum Pox Virus ◽  
Prunus Domestica

EXPRESSION OF THE PLUM POX VIRUS COAT PROTEIN REGION IN ESCHERICHIA COLI

1989 ◽  
pp. 131-132
Author(s):  
D. Mattanovich ◽  
G. Himmler ◽  
M. Laimer ◽  
A. da Camara Machado ◽  
V. Hanzer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Plum Pox Virus ◽  
Coat Protein Region ◽  
Virus Coat Protein ◽  
Virus Coat
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