Confirming and Identifying New Loci for Rice Blast Disease Resistance using Magnaporthe oryzae Field Isolates in the US

Crop Science ◽  
2015 ◽  
Vol 55 (6) ◽  
pp. 2620-2627 ◽  
Author(s):  
Junjie Xing ◽  
Melissa H Jia ◽  
James C. Correll ◽  
Longping Yuan ◽  
Huangfeng Deng ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Field Isolates ◽  
The Us

scholarly journals Role of two metacaspases in development and pathogenicity of the Rice Blast fungus, Magnaporthe oryzae

2020 ◽  
Author(s):  
Jessie Fernandez ◽  
Victor Lopez ◽  
Lisa Kinch ◽  
Mariel A. Pfeifer ◽  
Hillery Gray ◽  
...  
Keyword(s):  
Cell Death ◽  
Food Security ◽  
Life Cycle ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Complex Life Cycle ◽  
Insight Into

ABSTRACTRice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell death in M. oryzae during infection, we characterized two predicted M. oryzae metacaspase proteins, MoMca1 and MoMca2. These proteins appear to be functionally redundant and are able to complement the yeast Yca1 homologue. Biochemical analysis revealed that M. oryzae metacaspases exhibited Ca2+ dependent caspase activity in vitro. Deletion of both MoMca1 and MoMca2 in M. oryzae resulted in reduced sporulation, delay in conidial germination and attenuation of disease severity. In addition, the double ΔMomca1mca2 mutant strain showed increased radial growth in the presence of oxidative stress. Interestingly, the ΔMomca1mca2 strain showed an increase accumulation of insoluble aggregates compared to the wild-type strain during vegetative growth. Our findings suggest that MoMca1 and MoMca2 promote the clearance of insoluble aggregates in M. oryzae, demonstrating the important role these metacaspases have in fungal protein homeostasis. Furthermore, these metacaspase proteins may play additional roles, like in regulating stress responses, that would help maintain the fitness of fungal cells required for host infection.IMPORTANCEMagnaporthe oryzae causes rice blast disease that threatens global food security by resulting in the severe loss of rice production every year. A tightly regulated life cycle allows M. oryzae to disarm the host plant immune system during its biotrophic stage before triggering plant cell death in its necrotrophic stage. The ways M. oryzae navigates its complex life cycle remains unclear. This work characterizes two metacaspase proteins with peptidase activity in M. oryzae that are shown to be involved in the regulation of fungal growth and development prior to infection by potentially helping maintain fungal fitness. This study provides new insight into the role of metacaspase proteins in filamentous fungi by illustrating the delays in M. oryzae morphogenesis in the absence of these proteins. Understanding the mechanisms by which M. oryzae morphology and development promote its devastating pathogenicity may lead to the emergence of proper methods for disease control.

Microconidia: Understanding Its Role in the Fungus Magnaporthe oryzae Inciting Rice Blast Disease

2021 ◽  
pp. 143-150
Author(s):  
Ganesan Prakash ◽  
Asharani Patel ◽  
Ish Prakash ◽  
Kuleshwar Prasad Sahu ◽  
Rajashekara Hosahatti ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease

Morphological and molecular characterization of Magnaporthe oryzae causing rice blast disease in Odisha

2018 ◽  
Vol 55 (3) ◽  
pp. 467 ◽  
Author(s):  
Chinmayee Sahu ◽  
Manoj Kumar Yadav ◽  
Gayatree Panda ◽  
S Aravindan ◽  
Ngangkham Umakanta ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Morphological And Molecular Characterization

scholarly journals Effector-Mediated Suppression of Chitin-Triggered Immunity by Magnaporthe oryzae Is Necessary for Rice Blast Disease

2012 ◽  
Vol 24 (1) ◽  
pp. 322-335 ◽  
Author(s):  
Thomas A. Mentlak ◽  
Anja Kombrink ◽  
Tomonori Shinya ◽  
Lauren S. Ryder ◽  
Ippei Otomo ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease

Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pik h )

2010 ◽  
Vol 27 (1) ◽  
pp. 129-135 ◽  
Author(s):  
G. Ramkumar ◽  
K. Srinivasarao ◽  
K. Madhan Mohan ◽  
I. Sudarshan ◽  
A. K. P. Sivaranjani ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Functional Marker ◽  
Blast Disease ◽  
Disease Resistance Gene ◽  
Development And Validation

scholarly journals Equol, a Clinically Important Metabolite, Inhibits the Development and Pathogenicity of Magnaporthe oryzae, the Causal Agent of Rice Blast Disease

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1799 ◽  
Author(s):  
Jiaoyu Wang ◽  
Ling Li ◽  
Yeshi Yin ◽  
Zhuokan Gu ◽  
Rongyao Chai ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Causal Agent ◽  
Rice Blast Disease ◽  
Blast Disease

scholarly journals Role of Two Metacaspases in Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae

mBio ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jessie Fernandez ◽  
Victor Lopez ◽  
Lisa Kinch ◽  
Mariel A. Pfeifer ◽  
Hillery Gray ◽  
...  
Keyword(s):  
Cell Death ◽  
Food Security ◽  
Life Cycle ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Complex Life Cycle ◽  
Content Type

ABSTRACT Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell death in M. oryzae during infection, we characterized two predicted M. oryzae metacaspase proteins, MoMca1 and MoMca2. These proteins appear to be functionally redundant and can complement the yeast Yca1 homologue. Biochemical analysis revealed that M. oryzae metacaspases exhibited Ca2+-dependent caspase activity in vitro. Deletion of both MoMca1 and MoMca2 in M. oryzae resulted in reduced sporulation, delay in conidial germination, and attenuation of disease severity. In addition, the double ΔMomca1mca2 mutant strain showed increased radial growth in the presence of oxidative stress. Interestingly, the ΔMomca1mca2 strain showed an increased accumulation of insoluble aggregates compared to the wild-type strain during vegetative growth. Our findings suggest that MoMca1 and MoMca2 promote the clearance of insoluble aggregates in M. oryzae, demonstrating the important role these metacaspases have in fungal protein homeostasis. Furthermore, these metacaspase proteins may play additional roles, like in regulating stress responses, that would help maintain the fitness of fungal cells required for host infection. IMPORTANCE Magnaporthe oryzae causes rice blast disease that threatens global food security by resulting in the severe loss of rice production every year. A tightly regulated life cycle allows M. oryzae to disarm the host plant immune system during its biotrophic stage before triggering plant cell death in its necrotrophic stage. The ways M. oryzae navigates its complex life cycle remain unclear. This work characterizes two metacaspase proteins with peptidase activity in M. oryzae that are shown to be involved in the regulation of fungal growth and development prior to infection by potentially helping maintain fungal fitness. This study provides new insights into the role of metacaspase proteins in filamentous fungi by illustrating the delays in M. oryzae morphogenesis in the absence of these proteins. Understanding the mechanisms by which M. oryzae morphology and development promote its devastating pathogenicity may lead to the emergence of proper methods for disease control.

Recent Insights in Rice Blast Disease Resistance

2021 ◽  
pp. 89-123
Author(s):  
Susheel Kumar Sharma ◽  
Devender Sharma ◽  
Ram Prasnna Meena ◽  
Manoj Kumar Yadav ◽  
H. Rajashekara ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease
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