Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pik h )

2010 ◽  
Vol 27 (1) ◽  
pp. 129-135 ◽  
Author(s):  
G. Ramkumar ◽  
K. Srinivasarao ◽  
K. Madhan Mohan ◽  
I. Sudarshan ◽  
A. K. P. Sivaranjani ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Functional Marker ◽  
Blast Disease ◽  
Disease Resistance Gene ◽  
Development And Validation

scholarly journals Identification of Microsatellite (SSR) and RAPD Markers Linked to Rice Blast Disease Resistance gene in Rice (Oryza sativa L.)

2011 ◽  
Vol 10 (17) ◽  
pp. 3301-3321 ◽  
Author(s):  
Selvaraj C Immanuel ◽  
Nagarajan Pothiraj ◽  
K Thiyagarajan ◽  
M Bharathi ◽  
R Rabindran
Keyword(s):  
Oryza Sativa ◽  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Rapd Markers ◽  
Oryza Sativa L ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Disease Resistance Gene

Saturation mapping with subclones of YACs: DNA marker production targeting the rice blast disease resistance gene, Pi-b

1997 ◽  
Vol 94 (2) ◽  
pp. 170-176 ◽  
Author(s):  
L. Monna ◽  
A. Miyao ◽  
H. S. Zhong ◽  
M. Yano ◽  
M. Iwamoto ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Dna Marker ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Disease Resistance Gene ◽  
Saturation Mapping

scholarly journals Cloning and Function Analysis of a Novel Rice Blast Resistance Gene Pi65 in Japonica Rice

2021 ◽  
Author(s):  
Lili Wang ◽  
Zuobin Ma ◽  
Houxiang Kang ◽  
Shuang Gu ◽  
Zhanna Mukhina ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Resistant Variety ◽  
Japonica Rice ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

Abstract Rice blast seriously threatens rice production worldwide. Utilizing the rice blast resistance gene to breed the rice blast resistant varieties is one of the best ways to control rice blast disease. Using a map-based cloning strategy, here, we cloned a novel rice blast resistance gene, Pi65 from the resistant variety GangYu129 (abbreviated GY129, O. sativa japonica ). Overexpression of Pi65 in the susceptible variety LiaoXing1 (abbreviated LX1, O. sativa japonica ) enhanced rice blast resistance, while knockout of Pi65 in GY129 resulted in susceptible to rice blast disease. Pi65 encodes two transmembrane domains, with 15 LRR domains and one serine/threonine protein kinase catalytic domain, conferring resistance to isolates of M. oryzae collected from northeast China. There are sixteen amino acids differences between the Pi65 resistance and susceptible alleles. Compared with the Pi65 resistant allele, the susceptible allele deleted one LRR domain. Pi65 was constitutively expressed in whole plants, and it could be induce expressed in the early stage of M. oryzae infection . Transcriptome analysis revealed that numerous genes associated with disease resistance were specifically upregulated in GY129 24-hour post inoculation (HPI), on the contrary, the photosynthesis-and carbohydrate metabolism-related genes were particularly downregulated 24 HPI, demonstrating that the disease resistance associated genes has been activated in GY129 (carrying Pi65 ) after rice blast fungal infection, and the cellular basal and energy metabolism was inhibited simultaneously. Our study provides genetic resources for improving rice blast resistance as well as enriches the study of rice blast resistance mechanisms.

Recent Insights in Rice Blast Disease Resistance

2021 ◽  
pp. 89-123
Author(s):  
Susheel Kumar Sharma ◽  
Devender Sharma ◽  
Ram Prasnna Meena ◽  
Manoj Kumar Yadav ◽  
H. Rajashekara ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease

Confirming and Identifying New Loci for Rice Blast Disease Resistance using Magnaporthe oryzae Field Isolates in the US

Crop Science ◽  
2015 ◽  
Vol 55 (6) ◽  
pp. 2620-2627 ◽  
Author(s):  
Junjie Xing ◽  
Melissa H Jia ◽  
James C. Correll ◽  
Longping Yuan ◽  
Huangfeng Deng ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Field Isolates ◽  
The Us

scholarly journals Osa‐miR398b boosts H 2 O 2 production and rice blast disease‐resistance via multiple superoxide dismutases

2019 ◽  
Vol 222 (3) ◽  
pp. 1507-1522 ◽  
Author(s):  
Yan Li ◽  
Xiao‐Long Cao ◽  
Yong Zhu ◽  
Xue‐Mei Yang ◽  
Kai‐Ni Zhang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Superoxide Dismutases

A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice blast disease resistance

2008 ◽  
Vol 69 (3) ◽  
pp. 337-346 ◽  
Author(s):  
Hui Li ◽  
Shan-Yue Zhou ◽  
Wen-Sheng Zhao ◽  
Sheng-Chang Su ◽  
You-Liang Peng
Keyword(s):  
Disease Resistance ◽  
Protein Kinase ◽  
Rice Blast ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Kinase Gene ◽  
Protein Kinase Gene

scholarly journals A Blast Resistance Gene Pi65 with LRR-RLK Domain is Required for Resistance to M. Oryzae

2021 ◽  
Author(s):  
Li-Li Wang ◽  
Zuo-Bin Ma ◽  
Hou-Xiang Kang ◽  
Shuang Gu ◽  
Zhanna Mukhina ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Catalytic Domain ◽  
Blast Resistance ◽  
Resistant Variety ◽  
Disease Resistance Gene ◽  
Rice Varieties ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

Abstract Rice blast seriously threatens rice production worldwide. To control this disease, it is necessary to identify and utilize blast resistance genes to breed disease-resistant rice varieties. Here, we report a rice blast resistance gene, Pi65, isolated from the resistant variety GangYu129 (abbreviated GY129, O. sativa japonica) by map-based cloning. Pi65 overexpression in the susceptible variety LiaoXing1 (abbreviated LX1, O. sativa japonica) enhanced blast resistance, while Pi65 knockout in GY129 resulted in a decrease in its resistance to rice blast. Pi65 encodes two transmembrane regions, with 15 LRR domains and one serine/threonine protein kinase catalytic domain, conferring resistance to isolates of M. oryzae collected from northeast China. Sixteen amino acids differed between the resistance and susceptibility proteins. The Pi65 susceptibility allele had one fewer LRR duplication. Pi65 was constitutively expressed in whole plants, and M. oryzae inoculation significantly increased its expression level. Transcriptome sequencing revealed that numerous genes associated with disease resistance were specifically upregulated in GY129 24 h after M. oryzae inoculation, and photosynthesis-and carbohydrate metabolism-related genes were particularly downregulated, demonstrating disease resistance gene activation in GY129 mediated by Pi65 after rice blast fungal infection, cellular basal and energy metabolism was inhibited simultaneously. These combined factors endow GY129 with rice blast resistance. Our study provides genetic resources for improving rice blast resistance in japonica rice and enriches the study of rice blast resistance mechanisms.

Sign in / Sign up

Export Citation Format

Share Document