Germplasm Preservation

Somatic embryogenesis is one of the newest technology that applied for the mass production of cocoa. This research aims to evaluate the regeneration rate of somatic embryos through somatic embryogenesis propagation techniques on java fine flavor cocoa. Cultivars in this study are ICCRI 01, ICCRI 02, DR 1, DR 2, DRC 16, DR 38, PNT 16, and PNT 30. Observations include parameters to determine the percentage of primary callus and embryogenic callus formation and the number of somatic embryos produced. Based on data, the ability of callus to produce primary embryos is highly dependent on plant cultivars and explant sources. Five cultivars showed a higher regeneration rate using explants from the petal part, while the rest showed a higher regeneration rate using explants from the staminode section. Embryogenic callus from each cacao cultivar has the same basic structure: a nodular friable structure consisting of many embryonic cells. Some fine flavor cacao cultivars that were able to produce callus and primary somatic embryos could not produce secondary somatic embryos and plantlets. However, two cultivars, which had low potential in producing primary embryos, had the high ability to produce secondary somatic embryos and develop into plantlets.

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Germplasm Preservation And Propagation; The Foundation Of Agricultural Development –A Review

IOSR Journal of Pharmacy and Biological Sciences ◽

10.9790/3008-1104017073 ◽

2016 ◽

Vol 11 (04) ◽

pp. 70-73 ◽

Cited By ~ 1

Author(s):

Nwachukwu, Ifeanyi ◽

Ndukwe, Okorie k ◽

Ekaiko, Marshall. U.

Keyword(s):

Agricultural Development ◽

Germplasm Preservation

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AN ALTERNATIVE APPROACH TO GERMPLASM PRESERVATION

HortScience ◽

10.21273/hortsci.25.9.1179f ◽

1990 ◽

Vol 25 (9) ◽

pp. 1179f-1179

Author(s):

Kent Whealy

Keyword(s):

Apple Orchard ◽

Germplasm Preservation ◽

Plant Germplasm ◽

Alternative Approach ◽

Working Together ◽

User Groups ◽

National Plant Germplasm System

Seed Savers Exchange (SSE), founded in 1975, is a grassroots network of nearly 1,000 amateur growers who are working together to save heirloom vegetables and fruit varieties from extinction. In 1986 SSE purchased a 140-acre farm near Decorah, Iowa. Major projects either in place or under development at Heritage Farm include large Preservation Gardens where the seeds of 1,200 rare vegetables are multiplied each summer, Historic Apple Orchard of 600 old-time varieties, and endangered breeds of livestock and poultry. This talk will focus on the history and development of SSE, differences in the user groups serviced by the National Plant Germplasm System (NPGS) and SSE, the differing problems faced by both systems, and specific ways that NPGS and SSE can compliment each other's efforts.

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