Medium-term in vitro storage of pear as a complementary germplasm preservation technique

A technique for the long-term (up to 27 years) in vitro storage of valuable birch genotypes under normal (25 °C, 2.0 klx, 16-h day and 8-h night) and low temperature (4 °C, 0.5 klx, 6-h day and 18-h night) growing conditions on hormone-free media has been described. The study explored for the first time the influence of different strategies to store the clones of Betula pubescens and B. pendula var. сarelica (6 genotypes) on the regenerative capacity of collection samples, adaptive potential of regenerated plants and plant production by the in vitro and ex vitro techniques. It was established that both storage strategies provided a persistently high survival rate (82-100%) and regenerative capacity of in vitro shoots (the multiplication coefficient of 4.2-6.3 and rhizogenic activity of 90-100%). The clones retained their characteristics of height growth under the in vitro and ex vitro conditions, and demonstrated intraclonal homogeneity and lack of signs of somaclonal variability. The plants showed substantial interspecific differences at the stage of multiplication and transfer to the greenhouse. The highest percentage of acclimated plants (75-98% depending on the clone genotype) was obtained after planting of micro plants straight in the greenhouse, which simplified the technology and made plant production less costly. long-term in vitro storage, birch, species, genotype, micropropagation, ex vitro adaptation, plant material

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Cryopreservation of Woody Crops: The Avocado Case

Plants ◽

10.3390/plants10050934 ◽

2021 ◽

Vol 10 (5) ◽

pp. 934

Author(s):

Chris O’Brien ◽

Jayeni Hiti-Bandaralage ◽

Raquel Folgado ◽

Alice Hayward ◽

Sean Lahmeyer ◽

...

Keyword(s):

Tissue Culture ◽

Ex Situ Conservation ◽

Plant Genetic Resources ◽

Ex Situ ◽

Conservation Strategies ◽

Recalcitrant Seed ◽

In Vitro Storage ◽

Germplasm Collections ◽

Seed Crops

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

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Effect of Photosensitization Mediated by Curcumin on Carotenoid and Aflatoxin Content in Different Maize Varieties

Applied Sciences ◽

10.3390/app11135902 ◽

2021 ◽

Vol 11 (13) ◽

pp. 5902

Author(s):

Rafael Nguenha ◽

Maral Seidi Damyeh ◽

Anh D. T. Phan ◽

Hung T. Hong ◽

Mridusmita Chaliha ◽

...

Keyword(s):

Health Risks ◽

Carotenoid Content ◽

Naturally Occurring ◽

Maize Varieties ◽

Food And Feed ◽

Maize Kernels ◽

Dose Dependent ◽

Preservation Technique ◽

Scanning Electron

Mycotoxins are naturally occurring toxins produced by certain types of fungi that contaminate food and feed, posing serious health risks to human and livestock. This study evaluated the combination of blue light with curcumin to inactivate Aspergillus flavus spores, its effect on aflatoxin B1 (AFB1) production and maintaining carotenoid content in three maize varieties. The study was first conducted in vitro, and the spore suspensions (104 CFU·mL−1) were treated with four curcumin concentrations (25 and 50 µM in ethanol, 1000 and 1250 µM in propylene glycol) and illuminated at different light doses from 0 to 130.3 J·cm−2. The photoinactivation efficiency was light-dose dependent with the highest photoinactivation of 2.3 log CFU·mL−1 achieved using 1000 µM curcumin at 104.2 J·cm−2. Scanning electron microscopy revealed cell wall deformations as well as less density in photosensitized cells. Photosensitization of maize kernels gave rise to a complete reduction in the viability of A. flavus and therefore inhibition of AFB1 production, while no significant (p > 0.05) effect was observed using either light or curcumin. Moreover, photosensitization did not affect the carotenoids in all the studied maize varieties. The results suggest that photosensitization is a green alternative preservation technique to decontaminate maize kernels and reduce consumer exposure to AFB1 without any effect on carotenoid content.

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In vitro storage ofXanthosoma spp. under minimal growth conditions

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/bf00046088 ◽

1994 ◽

Vol 36 (3) ◽

pp. 309-316 ◽

Cited By ~ 9

Author(s):

E. A. Zandvoort ◽

M. J. H. Hulshof ◽

G. Staritsky

Keyword(s):

Growth Conditions ◽

Minimal Growth ◽

In Vitro Storage

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Genotype- and Sex-Specific Protocols for in vitro Micropropagation and Medium-Term Conservation of Jojoba

Biologia Plantarum ◽

10.1023/b:biop.0000024270.02186.1f ◽

2004 ◽

Vol 48 (1) ◽

pp. 19-23 ◽

Cited By ~ 14

Author(s):

R.K. Tyagi ◽

S. Prakash

Keyword(s):

Medium Term ◽

In Vitro Micropropagation

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The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

10.21203/rs.3.rs-597268/v1 ◽

2021 ◽

Author(s):

Paulo Hercilio Viegas Rodrigues ◽

Emerson Oliveira ◽

Christian Demetrio ◽

Guilherme Ambrosano ◽

Sônia Maria Stefano Piedade

Keyword(s):

Plant Material ◽

Slow Growth ◽

Shoot Cultures ◽

Ms Medium ◽

In Vitro Storage ◽

Light Spectra ◽

Slow Growth Storage ◽

C 50 ◽

Commercial Micropropagation

Abstract Maintaining updated in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delay and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra did not survive after 140 days of in vitro slow-growth storage. The plantlets maintained under the B spectrum survived after 140 days of in vitro slow-growth storage and showed little browning.

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