Bimodal Gigaporous Polystyrene Microspheres with Glycopolymer Surfaces for High-Speed Protein Chromatography

2021 ◽  
Author(s):  
Bingqi Zhu ◽  
Xinyi Sui ◽  
Yue Li ◽  
Huanjie Che ◽  
Jing Li ◽  
...  
Keyword(s):  
High Speed ◽  
Polystyrene Microspheres ◽  
Protein Chromatography

Hydrophilically Coated Gigaporous Polystyrene Microspheres and their Application in High-Speed Protein Chromatography

2011 ◽  
Vol 233-235 ◽  
pp. 1924-1929 ◽  
Author(s):  
Jian Bo Qu ◽  
Xiao Xiao Zhang ◽  
Guang Lun Jing ◽  
Wei Qing Zhou ◽  
Guang Hui Ma
Keyword(s):  
Flow Velocity ◽  
High Speed ◽  
High Flow ◽  
Low Back ◽  
Polystyrene Microspheres ◽  
Protein Chromatography ◽  
Hydrophobically Modified ◽  
Model Protein ◽  
Styrene Divinylbenzene ◽  
Exchange Medium

Gigaporous poly (styrene-divinylbenzene) (PS) microspheres have been effectively hydrophilized by coating with hydrophobically modified agarose (phenoxyl agarose, Agap). The effect of the phenoxy content on the amount of Agap adsorbed onto the PS surface was examined and various samples of microspheres, differing in surface Agap density, were prepared. After coating, the hydrophilicity of gigaporous PS microspheres was found to be greatly enhanced and the gigaporous character of PS microspheres was well maintained. The modified particles were further coupled with DEAE groups to prepare a DEAE anion exchange medium (DEAE-AP). A column packed with DEAE-AP showed low back pressure and high protein resolution at high flow velocity. Notably, the DEAE-AP column could completely separate a model protein mixture at a flow velocity of 3612 cm/h within 2 min. The results indicate that the gigaporous matrix has considerable advantages for high-speed protein chromatography.

Chemical coating polysaccharide on gigaporous polystyrene microspheres as a high-speed protein chromatography matrix

2016 ◽  
Vol 56 (12) ◽  
pp. 1407-1414 ◽  
Author(s):  
Jian-Bo Qu ◽  
Yu-Liang Xu ◽  
Jun-Yi Liu ◽  
Shi-Hai Li ◽  
Wei-Qing Zhou ◽  
...  
Keyword(s):  
High Speed ◽  
Polystyrene Microspheres ◽  
Protein Chromatography ◽  
Chemical Coating

Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support

2012 ◽  
Vol 72 (9) ◽  
pp. 606-612 ◽  
Author(s):  
Jian-Bo Qu ◽  
Hui-Hui Shao ◽  
Juan Li ◽  
Guang-Lun Jing ◽  
Jian-Guo Liu ◽  
...  
Keyword(s):  
High Speed ◽  
Polystyrene Microspheres ◽  
Protein Chromatography

A novel superporous agarose medium for high-speed protein chromatography

2005 ◽  
Vol 92 (5) ◽  
pp. 643-651 ◽  
Author(s):  
Qing-Hong Shi ◽  
Xin Zhou ◽  
Yan Sun
Keyword(s):  
High Speed ◽  
Protein Chromatography

Synthesis of Bimodal Open-Porous Polystyrene Monoliths with Glycopolymer Surfaces for High-Speed Protein Chromatography

2021 ◽  
Author(s):  
Jian-Bo Qu ◽  
Yang-Yang Lin ◽  
Yuan Liu ◽  
Bing-Qi Zhu ◽  
Yong-Jun Sun ◽  
...  
Keyword(s):  
High Speed ◽  
Protein Chromatography

Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix

2014 ◽  
Vol 6 (15) ◽  
pp. 12752-12760 ◽  
Author(s):  
Jian-Bo Qu ◽  
Guan-Sheng Huan ◽  
Yan-Li Chen ◽  
Wei-Qing Zhou ◽  
Jian-Guo Liu ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Polystyrene Microspheres ◽  
Protein Chromatography
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