Hydrophilically Coated Gigaporous Polystyrene Microspheres and their Application in High-Speed Protein Chromatography

2011 ◽  
Vol 233-235 ◽  
pp. 1924-1929 ◽  
Author(s):  
Jian Bo Qu ◽  
Xiao Xiao Zhang ◽  
Guang Lun Jing ◽  
Wei Qing Zhou ◽  
Guang Hui Ma
Keyword(s):  
Flow Velocity ◽  
High Speed ◽  
High Flow ◽  
Low Back ◽  
Polystyrene Microspheres ◽  
Protein Chromatography ◽  
Hydrophobically Modified ◽  
Model Protein ◽  
Styrene Divinylbenzene ◽  
Exchange Medium

Gigaporous poly (styrene-divinylbenzene) (PS) microspheres have been effectively hydrophilized by coating with hydrophobically modified agarose (phenoxyl agarose, Agap). The effect of the phenoxy content on the amount of Agap adsorbed onto the PS surface was examined and various samples of microspheres, differing in surface Agap density, were prepared. After coating, the hydrophilicity of gigaporous PS microspheres was found to be greatly enhanced and the gigaporous character of PS microspheres was well maintained. The modified particles were further coupled with DEAE groups to prepare a DEAE anion exchange medium (DEAE-AP). A column packed with DEAE-AP showed low back pressure and high protein resolution at high flow velocity. Notably, the DEAE-AP column could completely separate a model protein mixture at a flow velocity of 3612 cm/h within 2 min. The results indicate that the gigaporous matrix has considerable advantages for high-speed protein chromatography.

Chemical coating polysaccharide on gigaporous polystyrene microspheres as a high-speed protein chromatography matrix

2016 ◽  
Vol 56 (12) ◽  
pp. 1407-1414 ◽  
Author(s):  
Jian-Bo Qu ◽  
Yu-Liang Xu ◽  
Jun-Yi Liu ◽  
Shi-Hai Li ◽  
Wei-Qing Zhou ◽  
...  
Keyword(s):  
High Speed ◽  
Polystyrene Microspheres ◽  
Protein Chromatography ◽  
Chemical Coating

Hydrophilization of gigaporous polystyrene microspheres with saccharide as high-speed protein chromatography base support

2012 ◽  
Vol 72 (9) ◽  
pp. 606-612 ◽  
Author(s):  
Jian-Bo Qu ◽  
Hui-Hui Shao ◽  
Juan Li ◽  
Guang-Lun Jing ◽  
Jian-Guo Liu ◽  
...  
Keyword(s):  
High Speed ◽  
Polystyrene Microspheres ◽  
Protein Chromatography

Corrosion Rate Measurement by Hydrogen Effusion In Dynamic Aqueous Systems At Elevated Temperature and Pressure

CORROSION ◽  
1959 ◽  
Vol 15 (4) ◽  
pp. 29-32
Author(s):  
M. KRULFELD ◽  
M. C. BLOOM ◽  
R. E. SEEBOLD
Keyword(s):  
Elevated Temperature ◽  
Corrosion Rate ◽  
Flow Velocity ◽  
High Flow ◽  
Low Flow ◽  
Aqueous Systems ◽  
Effusion Rates ◽  
Temperature And Pressure ◽  
Effusion Method ◽  
Hydrogen Effusion

Abstract A method of applying the hydrogen effusion method to the measurement of corrosion rates in dynamic aqueous systems at elevated temperature and pressure is described. Data obtained in low carbon steel systems are presented, including (1) reproducibility obtained in measured hydrogen effusion rates at a flow velocity of 1 foot per second at a temperature of 600 F and 2000 psi, and (2) a quantitative comparison between the hydrogen effusion rates in static and in low flow velocity dynamic systems at this temperature and pressure. Some observations are included on corrosion rate measurements in a high flow velocity (30 feet per second) loop by the hydrogen effusion method. Implications of these measurements with regard to the comparison between high flow velocity corrosion and low flow velocity corrosion are mentioned and some data indicating high local sensitivity of the hydrogen effusion method are noted. Some possible difficulties involved in the method are pointed out. 2.3.4

Large area diamond reactor using a high flow velocity microwave plasma jet

1992 ◽  
Vol 1 (7) ◽  
pp. 814-817 ◽  
Author(s):  
D.K. Smith ◽  
E. Sevillano ◽  
M. Besen ◽  
V. Berkman ◽  
L. Bourget
Keyword(s):  
Flow Velocity ◽  
Microwave Plasma ◽  
Plasma Jet ◽  
High Flow ◽  
Large Area ◽  
High Flow Velocity

Fluttering Amplitude Amplification by Utilizing Flapping Moment in Flutter-Driven Triboelectric Nanogenerator

2021 ◽  
Author(s):  
Yi Zhang ◽  
Ka Chung Chan ◽  
Sau Chung Fu ◽  
Christopher Yu Hang Chao
Keyword(s):  
Flow Velocity ◽  
High Speed ◽  
Aerodynamic Force ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Open Circuit ◽  
Energy Output ◽  
Small Scale ◽  
Triboelectric Nanogenerator ◽  
Peak Value

Abstract Flutter-driven triboelectric nanogenerator (FTENG) is one of the most promising methods to harvest small-scale wind energy. Wind causes self-fluttering motion of a flag in the FTENG to generate electricity by contact electrification. A lot of studies have been conducted to enhance the energy output by increasing the surface charge density of the flag, but only a few researches tried to increase the converting efficiency by enlarging the flapping motion. In this study, we show that by simply replacing the rigid flagpole in the FTENG with a flexible flagpole, the energy conversion efficiency is augmented and the energy output is enhanced. It is found that when the flag flutters, the flagpole also undergoes aerodynamic force. The lift force generated from the fluttering flag applies a periodic rotational moment on the flagpole, and causes the flagpole to vibrate. The vibration of the flagpole, in turn amplifies the flutter of the flag. Both the fluttering dynamics of the flags with rigid and flexible flagpoles have been recorded by a high-speed camera. When the flag was held by a flexible flagpole, the fluttering amplitude and the contact area between the flag and electrode plates were increased. The energy enhancement increased as the flow velocity increased and the enhancement can be 113 times when the wind velocity is 10 m/s. The thickness of the flagpole was investigated. An optimal output of open-circuit voltage reaching 1128 V (peak-to-peak value) or 312.40 V (RMS value), and short-circuit current reaching 127.67 μA (peak-to-peak value) or 31.99 μA (RMS value) at 12.21 m/s flow velocity was achieved. This research presents a simple design to enhance the output performance of an FTENG by amplifying the fluttering amplitude. Based on the performance obtained in this study, the improved FTENG has the potential to apply in a smart city for driving electronic devices as a power source for IoT applications.

Evaluation of Intra-Aneurysmal Blood Flow: Investigation of the Flow in Experimental Aneurysms

1998 ◽  
Vol 4 (1_suppl) ◽  
pp. 77-80 ◽  
Author(s):  
F. Asakura ◽  
H. Tenjin ◽  
K. Matsumoto ◽  
S. Ueda
Keyword(s):  
Blood Flow ◽  
Flow Velocity ◽  
Coil Embolization ◽  
High Speed ◽  
Mean Transit Time ◽  
Digital Subtraction ◽  
High Speed Video ◽  
Tracer Particles ◽  
Flow Investigation ◽  
Platinum Coil

We evaluated the flow in experimental pig aneurysms using a high speed video system with tracer particles and digital subtraction angiography (DSA). We found that flow velocity in experimental aneurysms was fast on the inflow of aneurysms. There was a certain correlation between mean transit time (MTT) and velocity in experimental aneurysms. Therefore, the differences of flow velocity in aneurysms will be detected using DSA. The flow in aneurysms was much changed by platinum coil embolization.

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