scholarly journals Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants

BioTechniques ◽  
2003 ◽  
Vol 35 (3) ◽  
pp. 488-492 ◽  
Author(s):  
Susan D. Lawrence ◽  
Nicole G. Novak ◽  
Jeffrey M. Slack
Keyword(s):  
Monoclonal Antibody ◽  
Fusion Proteins ◽  
Epitope Tagging ◽  
Recombinant Fusion Proteins

Isolation of a Monoclonal Antibody to the TrpE Protein and Its Use for the Purification of Recombinant Fusion Proteins

Hybridoma ◽  
1991 ◽  
Vol 10 (6) ◽  
pp. 753-760 ◽  
Author(s):  
FAY NURSE ◽  
SHLOMO DAGAN ◽  
CHARLES TACKNEY ◽  
NEIL I. GOLDSTEIN
Keyword(s):  
Monoclonal Antibody ◽  
Fusion Proteins ◽  
Recombinant Fusion Proteins

Use of recombinant fusion proteins for generation and rapid characterization of monoclonal antibodies

1992 ◽  
Vol 147 (1) ◽  
pp. 1-11 ◽  
Author(s):  
D. Wunderlich ◽  
A. Lee ◽  
R.P. Fracasso ◽  
D.V. Mierz ◽  
R.M. Bayney ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fusion Proteins ◽  
Recombinant Fusion Proteins ◽  
Rapid Characterization

scholarly journals Epitope mapping by cDNA expression of a monoclonal antibody which inhibits the binding of von Willebrand factor to platelet glycoprotein IIb/IIIa

1992 ◽  
Vol 284 (3) ◽  
pp. 711-715 ◽  
Author(s):  
G Piétu ◽  
A S Ribba ◽  
G Chérel ◽  
D Meyer
Keyword(s):  
Monoclonal Antibody ◽  
Von Willebrand Factor ◽  
Fusion Proteins ◽  
Solid Phase ◽  
Platelet Glycoprotein ◽  
Rgd Sequence ◽  
Adhesive Proteins ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Beta Galactosidase

In order to study the structure-function relationship of von Willebrand Factor (vWF), we have located the epitope of a well-characterized monoclonal antibody (MAb) to vWF (MAb 9). This MAb reacts with the C-terminal portion of the vWF subunit, SPII fragment [amino acids (aa) 1366-2050], which includes an Arg-Gly-Asp (RGD) sequence at positions 1744-1746, and totally inhibits vWF and SPII binding to platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa). A recombinant DNA library was constructed by cloning small (250-500 nucleotides) vWF cDNA fragments into the lambda gt11 vector and these inserts were expressed as fusion proteins with beta-galactosidase. Immunological screening of the library with 125I-MAb 9 identified three immunoreactive clones. vWF inserts were amplified by the PCR and their sequences demonstrated overlapping nucleotides from positions 7630 to 7855 of vWF cDNA, coding for aa residues 1698-1773 of the mature subunit, indicating that this is the epitope of MAb 9. vWF-beta-galactosidase fusion protein reacted with 125I-MAb 9 by Western blotting. In a solid-phase radioimmunoassay, the purified fusion proteins decreased the binding of vWF to 125I-MAb 9 by 50%, and this inhibition was dose-dependent between 3.5 and 120 nM. Therefore the epitope of MAb 9 is located within aa 1698-1773 of the vWF subunit, which includes the RGD sequence implicated in the binding of adhesive proteins of GPIIb/IIIa.

scholarly journals New Opportunities in Glycan Engineering for Therapeutic Proteins

Antibodies ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 5
Author(s):  
Xiaotian Zhong ◽  
Aaron M. D’Antona ◽  
John J. Scarcelli ◽  
Jason C. Rouse
Keyword(s):  
Fusion Proteins ◽  
Therapeutic Proteins ◽  
Quality Attributes ◽  
Efficacy And Safety ◽  
Biological Functions ◽  
Next Generation ◽  
Lysosomal Degradation ◽  
Critical Quality Attributes ◽  
Recombinant Fusion Proteins ◽  
Antibody Diversification

Glycans as sugar polymers are important metabolic, structural, and physiological regulators for cellular and biological functions. They are often classified as critical quality attributes to antibodies and recombinant fusion proteins, given their impacts on the efficacy and safety of biologics drugs. Recent reports on the conjugates of N-acetyl-galactosamine and mannose-6-phosphate for lysosomal degradation, Fab glycans for antibody diversification, as well as sialylation therapeutic modulations and O-linked applications, have been fueling the continued interest in glycoengineering. The current advancements of the human glycome and the development of a comprehensive network in glycosylation pathways have presented new opportunities in designing next-generation therapeutic proteins.

Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins

2010 ◽  
pp. 349-367 ◽  
Author(s):  
Adam Charlton ◽  
Michael Zachariou
Keyword(s):  
Fusion Proteins ◽  
Site Specific ◽  
Recombinant Fusion Proteins

Glucose Dehydrogenase as Detector Protein of Recombinant Fusion-Proteins Directly in SDS Gels

2001 ◽  
pp. 66-68
Author(s):  
Christa Burger ◽  
Winfried Linxweiler ◽  
Oliver Pöschke ◽  
Andrea Wolf ◽  
Uwe Hofmann ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Glucose Dehydrogenase ◽  
Recombinant Fusion Proteins

Exploration of Recombinant Fusion Proteins YAPO and YAPL as Carrier Proteins for Glycoconjugate Vaccine Design against Streptococcus pneumoniae Infection

2020 ◽  
Vol 6 (8) ◽  
pp. 2181-2191
Author(s):  
Shaojie Feng ◽  
Chenghe Xiong ◽  
Guirong Wang ◽  
Subo Wang ◽  
Guoxia Jin ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Fusion Proteins ◽  
Vaccine Design ◽  
Carrier Proteins ◽  
Recombinant Fusion Proteins ◽  
Glycoconjugate Vaccine

scholarly journals Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using Sortase A and Elastin-like Polypeptides

2013 ◽  
Vol 52 (13) ◽  
pp. 3703-3708 ◽  
Author(s):  
Joseph J. Bellucci ◽  
Miriam Amiram ◽  
Jayanta Bhattacharyya ◽  
Dewey McCafferty ◽  
Ashutosh Chilkoti
Keyword(s):  
Fusion Proteins ◽  
Sortase A ◽  
Site Specific ◽  
Specific Modification ◽  
Recombinant Fusion Proteins

Recombinant fusion proteins A and protein G with glutathione S-transferase as reporter molecules

1991 ◽  
Vol 136 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Andrew M. Lew ◽  
Dianne J. Beck ◽  
Lynda M. Thomas
Keyword(s):  
Fusion Proteins ◽  
Protein G ◽  
Glutathione S Transferase ◽  
Recombinant Fusion Proteins
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