scholarly journals Sequences Comparison of Cytochrome Oxidase I Gene of Certain Species of Insects in Kurdistan with Other Locations in The World

2021 ◽  
Vol 14 (4) ◽  
pp. 79-90
Author(s):  
Janati, S. ◽  
Kahrizi, D. ◽  
Mirmoayedi, A. ◽  
Yari, K.
Keyword(s):  
Cytochrome Oxidase ◽  
Cytochrome Oxidase I ◽  
The World ◽  
Cytochrome Oxidase I Gene ◽  
I Gene

Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes

2020 ◽  
Vol 23 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Ratnapal Gandhi ◽  
Kamlesh K. Yadav ◽  
Prabhakargouda B. Patil ◽  
Pankaj Bihani ◽  
Bharat Char ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

scholarly journals Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR

2018 ◽  
Vol 60 (0) ◽  
Author(s):  
Evelynne Urzêdo Leão ◽  
David Marques de Almeida Spadotti ◽  
Kelly Cristina G. Rocha ◽  
Élison Fabricio B. Lima ◽  
Luciana Tavella ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Real Time ◽  
Real Time Pcr ◽  
Direct Sequencing ◽  
Cytochrome Oxidase I ◽  
Frankliniella Schultzei ◽  
Efficient Detection ◽  
Cytochrome Oxidase I Gene ◽  
I Gene

The Comparative Molecular Study between Bombycidae and Saturniidae Based on mtDNA RFLP and Cytochrome Oxidase I Gene Sequences: Implication for Molecular Evolution

1999 ◽  
Vol 54 (7-8) ◽  
pp. 587-594 ◽  
Author(s):  
Jae-Sam Hwang ◽  
Jin-Sung Lee ◽  
Tae-Won Goo ◽  
Hyun-Ah Kang ◽  
Hae-Ryong Sohn ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Study ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Confidence Limits ◽  
Sequence Identity ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene ◽  
Mtdna Rflp

Abstract Bombycidae, Saturniidae, mtDNA RFLP, Cytochrome Oxidase I Gene The phylogenetic relationships between Bombyx mori and Bombyx mandarina species of Bombycidae, and Antheraea yamamai and Antheraea pernyi species of Saturniidae were investigated based on mtDNA RFLP and cytochrome oxidase I gene. The sizes of the mtDNA of all the species were estimated at approximately 16 kbp ± 500 bp by total length of all the restricted fragments and no variation in size was recognized. Of the fourteen different restriction endonucleases used, BamHl, Hindlll, Pstl, EcoRl and Xbal showed RFLP. Among these, only Hindlll showed RFLP between B. mori and B. mandarina. A comparative analysis of sequences was also conducted with the mitochondrial cytochrome oxidase I genes of each species. The results indicated that B. mori shared a 97% , 85% and 87% sequence identity with B. mandarina, A. yamamai and A. pernyi, respectively. B. mandarina shared a 87% and 88% sequence identity with A. yamamai and A. penyi, respectively. A. yamamai shared 92% sequence identity with A. pernyi. The results of the phylogenetic analysis exhib­ited monophyly and confidence limits of more than 99% in all trees for both Bombycidae and Saturniidae.

scholarly journals Pengujian Dua Pasang Primer Universal Untuk Amplifikasi Gen Cytochrome Oxidase I Copepoda

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (1) ◽  
pp. 93
Author(s):  
Thalita C. P. Sumampow
Keyword(s):  
Cytochrome Oxidase ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Universal Primer ◽  
Cytochrome Oxidase I Gene ◽  
I Gene

Copepoda merupakan zooplankton kaya manfaat dengan diversitas yang sangat tinggi dan terdiri dari banyak spesies kriptik. Identifikasi cepat, akurat, dan hemat dapat dilakukan dengan menggunakan teknik DNA Barcoding. Kesuksesan teknik tersebut sangat dipengaruhi oleh penggunaan primer yang tepat. Tujuan penelitian ini adalah untuk menguji kemampuan dua pasang primer universal, yakni LCO1490-HCO2198 dan FF2d-FR1d, mengamplifikasi gen COI Copepoda. Dalam penelitian ini, pasangan primer LCO1490-HCO2198 tidak berhasil mengamplifikasi gen target. Sekuens-sekuens hasil amplifikasi menggunakan pasangan primer FF2d-FR1d diidentifikasi melalui BLAST. Hasil yang diperoleh menunjukan bahwa sekuens-sekuens tersebut memiliki persentase kemiripan sebesar 92% dengan bakteri Pandoraea pnomenusa. Melalui hasil yang didapatkan disimpulkan bahwa kedua pasangan primer universal LCO1490-HCO2198 dan FF2d-FR1d tidak cukup spesifik untuk amplifikasi gen cytochrome oxidase I Copepoda.Copepoda is a very beneficial and highly diverse zooplankton with many cryptic species. A fast, reliable, and affordable identification can be done through DNA Barcoding. The success of this technique is affected by the usage of correct primers. The aim of this research was to test the ability of two universal primer pairs, which were LCO1490-HCO2198 and FF2d-FR1d, amplifying COI gene of Copepoda. In this research, LCO1490-HCO2198 primer pairs weren’t able to amplify COI gene of Copepoda. Sequences which were successfully amplified using FF2d-FR1d primer pairs were identified through BLAST. The result shows that the sequences are 92% similar to bacteria named Pandoraea pnomenusa. It can be concluded that both primer pairs are not specific enough to amplify cytochrome oxidase I gene of Copepoda.

scholarly journals Figure 2: Phylogeographic, morphometric and taxonomic re-evaluation of the river sardine, Mesobola brevianalis (Boulenger, 1908) (Teleostei, Cyprinidae, Chedrini)

2020 ◽  
Author(s):  
Megan A Ridden ◽  
Roger Bills ◽  
Martin H Villet
Keyword(s):  
Cytochrome Oxidase ◽  
Southern Africa ◽  
Morphometric Analysis ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Cytochrome Oxidase I ◽  
Cytochrome Oxidase I Gene ◽  
I Gene

<div><p>The river sardine, <i>Mesobola</i><i> </i><i>brevianalis</i> (Boulenger, 1908), is the type species of <i>Mesobola</i> Howes, 1984. Standard phylogenetic analyses of partial sequences of the cytochrome oxidase I gene of individuals from populations across southern Africa that are currently identified as <i>M.</i><i> </i><i>brevianalis</i> showed that these populations represent four genetically distinct allopatric lineages. Furthermore, <i>Engraulicypris</i><i> </i><i>sardella</i> (Günther, 1868), the type species of <i>Engraulicypris</i> Günther, 1894, was convincingly nested amongst these clades. </p><p><br></p> <p>Fig 2. Truss network used for morphometric analysis, defined by ten landmarks <b>A–J</b>.</p></div>

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