The Use of Affinity Tags to Overcome Obstacles in Recombinant Protein Expression and Purification

2015 ◽  
Vol 22 (10) ◽  
pp. 885-892 ◽  
Author(s):  
Chinthaka Amarasinghe ◽  
Jian-Ping Jin
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Expression And Purification ◽  
Affinity Tags ◽  
Protein Expression And Purification

Recombinant protein expression and purification of Taq DNA polymerase v1

2021 ◽  
Author(s):  
Maira Rivera ◽  
Javiera Reyes ◽  
Javiera A Avilés ◽  
Amparo Núñez ◽  
Fernan Federici ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Dna Polymerase ◽  
Recombinant Protein Expression ◽  
Recombinant Expression ◽  
Storage Conditions ◽  
Expression And Purification ◽  
Taq Dna Polymerase ◽  
Protein Expression And Purification

This is a slightly modified and simplified version of a protocol by Thomas G.W. Graham et al, which is available at https://gitlab.com/tjian-darzacq-lab/bearmix and has been described in depth in the article 10.1371/journal.pone.0246647, for the recombinant expression of a E602D mutant of Taq DNA polymerase in pET-28a that is available in Addgene (Addgene plasmid # 166944 ; http://n2t.net/addgene:166944 ; RRID:Addgene_166944). The main goal of this protocol is to eliminate the use of large volumes for dialysis and potential issues with the protein crashing out of the solution due to the use of concentrators for buffer exchange of this enzyme into storage conditions.

scholarly journals The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112643 ◽  
Author(s):  
Theodoros Goulas ◽  
Anna Cuppari ◽  
Raquel Garcia-Castellanos ◽  
Scott Snipas ◽  
Rudi Glockshuber ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Recombinant Protein Expression ◽  
Expression And Purification ◽  
System A ◽  
Protein Expression And Purification
Sign in / Sign up

Export Citation Format

Share Document