The Role of and the Place of Method Validation in Drug Analysis Using Electroanalytical Techniques

Fifteen cases of presumed cocaine intoxication were evaluated in the emergency room (ER) at a city hospital over a four-day period. This series is unique in that many of these patients were from a similar area of the city, in some cases had the same street address, were regular abusers of cocaine, and presented to the ER with similar symptoms of tachycardia, dilated pupils, marked confusion, bizarre and sometimes violent behavior, psychosis, and hallucinations. Many of these symptoms were present several hours after drug use. Samples of a white powder presumed by the patients to be cocaine were obtained from two patients and analyzed by gas-liquid chromatography. Neither sample contained cocaine, but rather revealed atropine, benzocaine, and procaine. The signs and symptoms of cocaine, amphetamine, and atropine intoxication are reviewed and the problems of drug analysis and differential diagnosis of drug intoxication are discussed.

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DRAXIN as a Novel Diagnostic Marker to Predict the Poor Prognosis of Glioma Patients

10.21203/rs.3.rs-636447/v1 ◽

2021 ◽

Author(s):

Zhendong Liu ◽

Runze Liu ◽

Xingbo Cheng ◽

Binfeng Liu ◽

Yongjie Zhu ◽

...

Keyword(s):

Poor Prognosis ◽

Clinical Information ◽

Drug Analysis ◽

Bioinformatics Analyses ◽

Carcinogenic Effect ◽

Tissue Samples ◽

Reverse Transcription Quantitative Pcr ◽

Biological Target ◽

The Relationship

Abstract An increasing number of evidences have shown that the carcinogenic effect of DRAXIN plays an important role in the malignant process of tumors, but the mechanism of its involvement in glioma has not yet been revealed. The main aim of this study is to explore the relationship between DRAXIN and the prognosis and pathogenesis of glioma through a large qualities of data analysis. Firstly, thousands of tissue samples with clinical information were collected based on various public databases. Then, a series of bioinformatics analyses were performed to mine data from information of glioma samples extracted from several reputable databases to reveal the key role of DRAXIN in glioma development and progression, with the confirmation of basic experiments. Our results showed high expression of the oncogene DRAXIN in tumor tissue and cells could be used as an independent risk factor for poor prognosis in glioma patients and was strongly associated with clinical risk features. The reverse transcription-quantitative PCR technique was then utilized to validate the DRAXIN expression results we obtained. In addition, co-expression analysis identified respectively top 10 genes that were closely associated with DRAXIN positively or negatively. Finally, four drugs that may have inhibitory effects on DRAXIN were gained by Cmap drug analysis. To sum up, this is the first report of DRAXIN being highly expressed in gliomas and leading to poor prognosis of glioma patients. DRAXIN may not only benefit to explore the pathogenesis of gliomas, but also serve as a novel biological target for the treatment of glioma.

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