scholarly journals PAK4 pathway as a potential therapeutic target in pancreatic cancer

2018 ◽  
Vol 14 (7) ◽  
pp. 579-582 ◽  
Author(s):  
Kiruthikah Thillai ◽  
Debashis Sarker ◽  
Claire Wells
2021 ◽  
Vol 12 (10) ◽  
Author(s):  
Xiao-ren Zhu ◽  
Shi-qing Peng ◽  
Le Wang ◽  
Xiao-yu Chen ◽  
Chun-xia Feng ◽  
...  

AbstractPancreatic cancer is the third leading cause of cancer-related mortalities and is characterized by rapid disease progression. Identification of novel therapeutic targets for this devastating disease is important. Phosphoenolpyruvate carboxykinase 1 (PCK1) is the rate-limiting enzyme of gluconeogenesis. The current study tested the expression and potential functions of PCK1 in pancreatic cancer. We show that PCK1 mRNA and protein levels are significantly elevated in human pancreatic cancer tissues and cells. In established and primary pancreatic cancer cells, PCK1 silencing (by shRNA) or CRISPR/Cas9-induced PCK1 knockout potently inhibited cell growth, proliferation, migration and invasion, and induced robust apoptosis activation. Conversely, ectopic overexpression of PCK1 in pancreatic cancer cells accelerated cell proliferation and migration. RNA-seq analyzing of differentially expressed genes (DEGs) in PCK1-silenced pancreatic cancer cells implied that DEGs were enriched in the PI3K-Akt-mTOR cascade. In pancreatic cancer cells, Akt-mTOR activation was largely inhibited by PCK1 shRNA, but was augmented after ectopic PCK1 overexpression. In vivo, the growth of PCK1 shRNA-bearing PANC-1 xenografts was largely inhibited in nude mice. Akt-mTOR activation was suppressed in PCK1 shRNA-expressing PANC-1 xenograft tissues. Collectively, PCK1 is a potential therapeutic target for pancreatic cancer.


Theranostics ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2141-2157 ◽  
Author(s):  
Qing Wang ◽  
Mengge Li ◽  
Yu Gan ◽  
Shuheng Jiang ◽  
Jie Qiao ◽  
...  

2010 ◽  
Vol 28 (15_suppl) ◽  
pp. e14614-e14614
Author(s):  
H. Miller ◽  
K. Ramachandran ◽  
E. Gordian ◽  
C. S. Rocha Lima ◽  
R. Singal

2018 ◽  
Vol 19 (4) ◽  
pp. 316-327 ◽  
Author(s):  
Rajani Kanteti ◽  
Tamara Mirzapoiazova ◽  
Jacob J. Riehm ◽  
Immanuel Dhanasingh ◽  
Bolot Mambetsariev ◽  
...  

2010 ◽  
Author(s):  
Kazim Husain ◽  
Domenico Coppola ◽  
Barbara Centeno ◽  
James Helm ◽  
John Kooman ◽  
...  

2011 ◽  
Author(s):  
Clifford Whatcott ◽  
Aprill Watanabe ◽  
Joseph Demirjian ◽  
Michael Barrett ◽  
Galen Hostetter ◽  
...  

Pancreas ◽  
2015 ◽  
Vol 44 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Jose M. Pimiento ◽  
Dung-Tsa Chen ◽  
Barbara A. Centeno ◽  
Ashley H. Davis-Yadley ◽  
Kazim Husain ◽  
...  

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