Moss polsters were collected at 15 sites between Toronto and Lake Timagami, Ontario, and at 4 localities in the Lake Timagami area for the purpose of determining the regional pollen rain and its local variations. Pollen percentages of Acer, Quercus, Ambrosia, Chenopodiaceae, Compositae, and Gramineae decrease northward and Picea, Pinus, and Betula increase at the more northerly sites. From the three most northern sites a regional pollen rain was calculated by averaging the percentages from nine pollen spectra. In this area the regional pollen rain is dominated by Picea (15%), Pinus (38%), and Betula (22%). At one site a grain of Ephedra was recovered, apparently carried in by long range drift. The nearest place that it grows naturally is in the southwestern United States. Various pollen trap types were investigated and it was found that all types of moss polsters and some types of decaying stumps (depending on their moisture-holding capacity) were effective in preserving the modern pollen rain.