scholarly journals Vitality and in vitro pollen germination of different ‘Oblacinska’ sour cherry clones

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 791-800
Author(s):  
Milica Fotiric-Aksic ◽  
Radosav Cerovic ◽  
Vera Rakonjac ◽  
Ivana Bakic ◽  
Slavica Colic ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Sour Cherry ◽  
Tube Growth ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Pollen Tube Length

Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two ?Oblacinska? sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23?C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 ?m (clone XIII/1, after 1 h) to >1,100 ?m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 ?m min-1) after 6 h of germination, but rather decreasing until 24 h of germination (0.56-0.83 ?m min-1). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).

scholarly journals The effect of temperature on pollen germination and pollen tube growth of pear cultivars

2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Different Temperatures ◽  
Pollen Tube Length

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.

GENOTYPIC DIFFERENCES IN CARDINAL TEMPERATURES FOR IN VITRO POLLEN GERMINATION AND POLLEN TUBE GROWTH OF COCONUT HYBRIDS

2017 ◽  
Vol 54 (5) ◽  
pp. 731-743 ◽  
Author(s):  
C. S. RANASINGHE ◽  
M. D. P. KUMARATHUNGE ◽  
K. G. S. KIRIWANDENIYA
Keyword(s):  
High Temperature ◽  
Sri Lanka ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Tube Growth ◽  
Tube Length ◽  
Cardinal Temperatures ◽  
Pollen Tube Length

SUMMARYSuccessful fruit set in coconut depends on several reproductive processes including pollen germination and pollen tube growth. High temperature (˃33 °C) during flowering reduces fruit set in coconut. Therefore, identification and development of coconut varieties or hybrids with high reproductive heat tolerance will benefit the coconut industry in view of the climate changes. This experiment was conducted to quantify the response of pollen germination and pollen tube growth of seven coconut hybrids to increasing temperature from 16 to 38 °C. A Principal Component Analysis (PCA) was carried out to classify coconut hybrids on the basis of their temperature tolerances to pollen germination. Pollen germination and pollen tube length of the hybrids ranged from 56 to 78% and 242 to 772 µm, respectively. A modified bilinear model best described the response to temperature of pollen germination and pollen tube length. Cardinal temperatures (Tmin, Topt and Tmax) of pollen germination and pollen tube length varied among the seven hybrids. PCA identified Tmax for pollen germination and Topt for pollen tube length as the most important parameters in describing varietal tolerance to high temperature. PCA also identified SLGD × Sri Lanka Tall and Sri Lanka Brown Dwarf × Sri Lanka Tall as the most tolerant hybrids to high temperature stress and Sri Lanka Tall × Sri Lanka Tall and Sri Lanka Green Dwarf × San Ramon as less tolerant ones based on cardinal temperatures for pollen germination and pollen tube length. Tmax for pollen germination of the most tolerant and less tolerant hybrids were 41.9 and 39.5 °C, respectively. Topt for pollen tube length in the most tolerant and less tolerant hybrids were 29.5 and 26.0 °C, respectively.

scholarly journals Effects of Ca2+ on in vitro pollen germination of three Acacia species

2016 ◽  
Vol 65 (2) ◽  
pp. 11-16 ◽  
Author(s):  
Ni Zhan ◽  
Liejian Huang
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Acacia Mangium ◽  
Germination Percentage ◽  
Tube Growth ◽  
Acacia Auriculiformis ◽  
Tube Length ◽  
In Vitro Pollen Germination

Abstract We investigated the effects of Ca2+ on in vitro pollen germination of Acacia auriculiformis, Acacia mangium, and Acacia crassicarpa under different concentrations of Ca2+ (50 mg.L−1, 100 mg.L−1, 150 mg.L−1, 200 mg.L−1, 250 mg.L−1, 300 mg.L−1, 350 mg.L−1 and 400 mg.L−1) in the present study. Our results revealed that Ca2+ could stimulate the pollen germination percentage and the pollen tube growth of A. auficuliformis and A. crassicarpa under a certain concentration. In vitro pollen germination of A. mangium did not require exogenous Ca2+. With high Ca2+ concentration, the pollen germination of A. auriculiformis, A. mangium, and A. crassicarpa were obviously inhibited. With the rise of Ca2+ concentration, the percentage of pollen germination, the pollen tube length, the pollen tube growth, and the number of pollen tube were increased. However, the pollen germination percentage decreased with high Ca2+ concentration.

scholarly journals Differences in in vitro Pollen Germination and Pollen Tube Growth of Cotton Cultivars in Response to High Temperature

2005 ◽  
Vol 96 (1) ◽  
pp. 59-67 ◽  
Author(s):  
V. G. KAKANI ◽  
K. R. REDDY ◽  
S. KOTI ◽  
T. P. WALLACE ◽  
P. V. V. PRASAD ◽  
...  
Keyword(s):  
High Temperature ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
In Vitro Pollen Germination

In vitro germination and pollen tube growth of maize (Zea mays) pollen. II. Pollen source, calcium, and boron interactions

1968 ◽  
Vol 46 (3) ◽  
pp. 235-240 ◽  
Author(s):  
P. L. Pfahler
Keyword(s):  
Zea Mays ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Germination Percentage ◽  
Tube Growth ◽  
Tube Length ◽  
Pollen Tube Length

Pollen grains from six single cross hybrids (Wf9 × H55, Wf9 × H50, H49 × H55, H49 × H50, Ky49 × Ky27, and K64 × K55) of Zea mays L. were cultured on an artificial medium containing 15% sucrose and 0.6% bacto-agar supplemented with all possible combinations of two levels (0.00 and 0.03%) of calcium nitrate (Ca) and two levels (0.00 and 0.01%) of boric acid (B). The effect of Ca and B on germination percentage was found to be dependent on the genetic source of the pollen. At the 0.00% levels of Ca and B, the germination percentage of the hybrids ranged from 26.6% for Ky49 × Ky27 to 58.1% for K64 × K55. With either Ky49 × Ky27 or K64 × K55 pollen, the addition of Ca and B to the medium resulted in a lower germination percentage whereas the addition of Ca and B increased the germination percentage of the other hybrids. The addition of B in the absence of Ca sharply reduced the germination percentage in all hybrids while in the presence of Ca, the effect of B varied with the hybrid involved. For pollen tube length, highly significant interactions between hybrids and levels of Ca and B were found. However, maximum pollen tube lengths were obtained for all hybrids with both Ca and B in the medium. The addition of B in the absence of Ca reduced the pollen tube length of all hybrids whereas the addition of B in the presence of Ca increased pollen tube length. In general, the addition of both Ca and B increased the rate of pollen tube growth especially in the period 2 to 3 hours after inoculation.

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