scholarly journals Direct Cardiac Reprogramming for Cardiovascular Regeneration and Differentiation

2020 ◽  
Vol 69 (3) ◽  
pp. 49-58 ◽  
Author(s):  
Taketaro Sadahiro ◽  
Masaki Ieda
Keyword(s):  
Cardiovascular Regeneration

Abstract P011: Endothelial Differentiation and Lineage Tracing in Adipocyte-Derived Multipotent Cells

2011 ◽  
Vol 109 (suppl_1) ◽  
Author(s):  
Medet Jumabay ◽  
Raushan Abdmaulen ◽  
Yucheng Yao ◽  
Kristina Bostrom
Keyword(s):  
Stem Cell ◽  
Lineage Tracing ◽  
Collagen Gels ◽  
Cellular Origin ◽  
Differentiated Cells ◽  
Stem Cell Source ◽  
Cardiovascular Regeneration ◽  
Morphogenetic Protein ◽  
Dfat Cells

We previously showed that so-called de-differentiated fat (DFAT) cells, which are derived from mature white adipocytes, spontaneously differentiate into beating cardiomyocytes. Our aim in this study was to investigate if DFAT cells also differentiate into endothelial cells (ECs) in vitro, and to further examine the cellular origin of DFAT cells as well as adipose stromal cells (ASCs) using lineage tracing. First, we examined DFAT and ASCs prepared from aP2-Cre+/+;LacZ ROSA(R26R)+/+ double transgenic mice, which express LacZ under the aP2 promoter. The results revealed that 99.9% of DFAT cells and 45% of the ASCs stained positive for LacZ, supporting that the DFAT cells and part of the ASCs are of adipocytic origin. Second, we allowed newly isolated DFAT cells to spontaneously undergo EC differentiation, which was monitored by expression of EC lineage markers as determined by real-time PCR, immunofluorescence, and FACS. Expression of the EC markers CD31 and VE-cadherin increased progressively during 2 weeks in culture, the percentage of CD31(+) cells increased from 0.0% to 8.3%, and the cells formed multi-cellular tube structures when placed in Matrigel™/Collagen gels. The data supported that a fraction of the DFAT cells differentiate into ECs. Furthermore, the EC differentiation could be enhanced in DFAT cells by treatment with bone morphogenetic protein (BMP)-4 and BMP-9. In addition to EC differentiation, the DFAT cells also expressed markers of other cardiovascular lineages including smooth muscle cells and pericytes. The multipotency of DFAT cells suggests that cellular de-differentiation might be a way for differentiated cells to regain stem cell-like properties. Thus, white mature adipocytes maybe a new stem cell source for cardiovascular regeneration.

Pluripotent Reprogramming and Lineage Reprogramming: Promises and Challenges in Cardiovascular Regeneration

2014 ◽  
Vol 20 (4) ◽  
pp. 304-313 ◽  
Author(s):  
Wen-Jun He ◽  
Qian Hou ◽  
Qing-Wang Han ◽  
Wei-Dong Han ◽  
Xiao-Bing Fu
Keyword(s):  
Cardiovascular Regeneration ◽  
Lineage Reprogramming

scholarly journals Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration

2007 ◽  
Vol 120 (10) ◽  
pp. 1791-1800 ◽  
Author(s):  
K. Tateishi ◽  
E. Ashihara ◽  
N. Takehara ◽  
T. Nomura ◽  
S. Honsho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Stem Cells ◽  
Cardiovascular Regeneration

scholarly journals Cardiovascular regeneration

2014 ◽  
Vol 5 (6) ◽  
pp. 141 ◽  
Author(s):  
Ronald A Li
Keyword(s):  
Cardiovascular Regeneration

Purification and Long-Term Expansion of Multipotent Endothelial-Like Cells with Potential Cardiovascular Regeneration

2012 ◽  
Vol 21 (4) ◽  
pp. 562-574 ◽  
Author(s):  
Juan A. Marchal ◽  
Manuel Picón ◽  
Macarena Perán ◽  
Clara Bueno ◽  
Manuel Jiménez-Navarro ◽  
...  
Keyword(s):  
Cardiovascular Regeneration
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