Immunocytolocalization Study of the External Covering of Trichinella spiralis Muscle Larva

1988 ◽  
Vol 74 (2) ◽  
pp. 270 ◽  
Author(s):  
Yuzo Takahashi ◽  
Takako Uno ◽  
Toshimasa Nishiyama ◽  
Shoji Yamada ◽  
Tsuneji Araki
Keyword(s):  
Trichinella Spiralis ◽  
Muscle Larva ◽  
External Covering

Trichinella spiralis: Proteins and antigens isolated from a large-particle fraction derived from the muscle larva

1981 ◽  
Vol 51 (2) ◽  
pp. 279-295 ◽  
Author(s):  
Dickson D. Despommier ◽  
Anthony Laccetti
Keyword(s):  
Large Particle ◽  
Trichinella Spiralis ◽  
Muscle Larva ◽  
Particle Fraction

Trichinella spiralis: Growth of the intracellular (muscle) larva

1975 ◽  
Vol 37 (1) ◽  
pp. 108-116 ◽  
Author(s):  
Dickson Despommier ◽  
Lorna Aron ◽  
Livia Turgeon
Keyword(s):  
Trichinella Spiralis ◽  
Muscle Larva

Trichinella spiralis : X-ray analysis of the nurse cell-muscle larva complex after exponential growth of the larva

1996 ◽  
Vol 82 (8) ◽  
pp. 731-736 ◽  
Author(s):  
M. J. Wranicz ◽  
H.-W. Koyro ◽  
R. Stelzer ◽  
M. Stoye
Keyword(s):  
Exponential Growth ◽  
Nurse Cell ◽  
Trichinella Spiralis ◽  
Muscle Larva ◽  
X Ray

Guanine-Cytosine Content of DNA from the Mature Muscle Larva of Trichinella spiralis as Determined from Buoyant Density and Thermal-Helix Coil Transition Measurements

1975 ◽  
Vol 61 (3) ◽  
pp. 570 ◽  
Author(s):  
Alan Feldman ◽  
Herbert S. Rosenkrantz ◽  
Dickson Despommier
Keyword(s):  
Trichinella Spiralis ◽  
Buoyant Density ◽  
Muscle Larva ◽  
Coil Transition

Detection of circulating Trichinella spiralis muscle larva antigens in serum samples of experimentally and naturally infected swine

1995 ◽  
Vol 58 (4) ◽  
pp. 319-326 ◽  
Author(s):  
C. Arriaga ◽  
L. Yépez-Mulia ◽  
A. Morilla ◽  
G. Ortega-Pierfes
Keyword(s):  
Trichinella Spiralis ◽  
Muscle Larva ◽  
Serum Samples

scholarly journals Molecular characterization of a Trichinella spiralis serine proteinase

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Xin Yue ◽  
Xiang Yuan Sun ◽  
Fang Liu ◽  
Chen Xi Hu ◽  
Ying Bai ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Infective Larva ◽  
Serine Proteinase ◽  
Secretory Protein ◽  
Muscle Larva ◽  
Target Antigen ◽  
Potential Candidate ◽  
Humoral Immune ◽  
Candidate Target ◽  
Cellular Immune

Abstract The aim of this study was to investigate the biological characteristics and functions of a Trichinella spiralis serine proteinase (TsSerp) during larval invasion and development in the host. The full-length TsSerp cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and western blotting analyses showed that TsSerp was a secretory protein that was highly expressed at the T. spiralis intestinal infective larva and muscle larva stages and primarily located at the cuticle, stichosome and intrauterine embryos of the parasite. rTsSerp promoted the larval invasion of intestinal epithelial cells (IECs) and the enteric mucosa, whereas an anti-rTsSerp antibody impeded larval invasion; the promotion and obstruction roles were dose-dependently related to rTsSerp and the anti-rTsSerp antibodies, respectively. Vaccination of mice with rTsSerp elicited a remarkable humoral immune response (high levels of serum IgG, IgG1/IgG2a, IgE and IgM), and it also triggered both systemic (spleen) and local intestinal mucosal mesenteric lymph node (MLN) cellular immune responses, as demonstrated by a significant elevation in Th1 cytokines (IFN-γ) and Th2 cytokines (IL-4) after the spleen and MLN cells from vaccinated mice were stimulated with rTsSerp. Anti-TsSerp antibodies participated in the killing and destruction of newborn larvae via ADCC. The mice vaccinated with rTsSerp exhibited a 48.7% reduction in intestinal adult worms and a 52.5% reduction in muscle larvae. These results indicated that TsSerp participates in T. spiralis invasion and development in the host and might be considered a potential candidate target antigen to develop oral polyvalent preventive vaccines against Trichinella infection.

Lenticular junctions: a form of cell contact between hypodermal cells in the nematode Trichinella spiralis

1987 ◽  
Vol 65 (3) ◽  
pp. 771-775 ◽  
Author(s):  
K. A. Wright ◽  
H. Hong ◽  
R. R. Shivers
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Intercellular Space ◽  
Trichinella Spiralis ◽  
Freeze Fracture ◽  
Intercellular Junctions ◽  
Muscle Larva ◽  
Dense Material ◽  
Cell Contact ◽  
Fracture Face

The intercellular junctions between hypodermal cells of the muscle larva of the nematode Trichinella spiralis were examined by thin section and freeze-fracture techniques of electron microscopy. The spacing between membranes varied greatly and the intercellular space contained dense material. In freeze-fractures, the P-fracture face had very few particles (especially compared with the rest of the lateral membranes), and possessed raised areas of an approximately lenticular shape. These areas were not outlined by particles. It is speculated that the form of the membranes is maintained by domains of phospholipids of conical and inverted cone shapes. These junctions likely have adhering and occluding functions.

Trichinella spiralis: The in situ localization of muscle larva antigens recognized by humans

1990 ◽  
Vol 70 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Yuzo Takahashi ◽  
Takako Uno ◽  
Naoto Mizuno ◽  
Hidekazu Suzuki ◽  
Kimitaka Shimazu ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Muscle Larva
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