The Occurrence of Anopheles darlingi Root in British Honduras and Guatemala

A biodiversidade da Região Amazônica inclui diversas espécies de vetores artrópodes em seus diferentes ecótopos, possibilitando o surgimento de doenças como malária, filarioses e arboviroses. De agosto de 2001 a julho de 2002, realizou-se coletas de culicídeos no domicílio, peridomicílio e nas matas da Comunidade São João, área rural de Manaus, Amazonas. Foram capturados 1.240 culicídeos, pertencentes às subfamílias Culicinae (99%) e Anophelinae (1%), somando 50 espécies. O predomínio entre as tribos foi nitidamente de Culicini, com 904 (72,9%) exemplares, destacando-se as espécies Culex usquatus (22,6%) e Culex quinquefasciatus (17,7%). Do total de culicídeos, 1.077 (86,9%) exemplares foram capturados no interior das matas, 101 (8,1%) no peridomicílio e 62 (5%) no intradomicílio. O ecótopo com maior diversidade de espécies foi a mata. Assinalou-se a presença de Anopheles darlingi, Anopheles triannulatus, Aedes aegypti, Haemagogus janthinomys e outros vetores comprovados ou potenciais.

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The Pattern of a Dependent Economy. A Study of the National Income of British Honduras

Revue économique ◽

10.2307/3497845 ◽

1954 ◽

Vol 5 (4) ◽

pp. 661

Author(s):

G. Hosmalin ◽

N. S. Carey Jones

Keyword(s):

National Income ◽

British Honduras

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American trypanosomiasis in British Honduras

Annals of Tropical Medicine and Parasitology ◽

10.1080/00034983.1971.11686743 ◽

1971 ◽

Vol 65 (2) ◽

pp. 179-183 ◽

Cited By ~ 1

Author(s):

W. B. Petana

Keyword(s):

American Trypanosomiasis ◽

British Honduras

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Anopheles darlingi versus Nyssorhynchus darlingi, the discussion continues

Trends in Parasitology ◽

10.1016/j.pt.2021.07.014 ◽

2021 ◽

Author(s):

Carlos J.E. Lamas ◽

Silvio S. Nihei ◽

Peter G. Foster ◽

Jan E. Conn ◽

Maria A.M. Sallum

Keyword(s):

Anopheles Darlingi

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Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential

Malaria Journal ◽

10.1186/s12936-020-03574-1 ◽

2021 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Elerson Matos Rocha ◽

Osvaldo Marinotti ◽

Deidre Machado Serrão ◽

Laura Viana Correa ◽

Ricardo de Melo Katak ◽

...

Keyword(s):

Public Health Problem ◽

Major Public Health Problem ◽

Culturable Bacteria ◽

Anopheles Darlingi ◽

Next Generation ◽

Bacterial Strains ◽

Promising Alternative ◽

Breeding Sites ◽

Adult Females ◽

Microbiological Techniques

Abstract Background Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.

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