TRUE Gene Silencing: Unique Gene Silencing by the tRNA Maturase tRNase ZL under the Direction of Small-guide RNA

2007 ◽  
Vol 49 (1) ◽  
pp. 54-64
Author(s):  
Aiko Nakashima ◽  
Masayuki Nashimoto ◽  
Masato Tamura
Keyword(s):  
Gene Silencing ◽  
Guide Rna ◽  
Unique Gene

TRUE Gene Silencing : Unique Gene Silencing by the tRNA Maturase tRNase ZL under the Direction of Small-guide RNA

2007 ◽  
Vol 49 (1) ◽  
pp. 54-64
Author(s):  
Aiko Nakashima ◽  
Masayuki Nashimoto ◽  
Masato Tamura
Keyword(s):  
Gene Silencing ◽  
Guide Rna ◽  
Unique Gene

scholarly journals Unique Gene-Silencing and Structural Properties of 2′-Fluoro-Modified siRNAs

2011 ◽  
Vol 50 (10) ◽  
pp. 2284-2288 ◽  
Author(s):  
Muthiah Manoharan ◽  
Akin Akinc ◽  
Rajendra K. Pandey ◽  
June Qin ◽  
Philipp Hadwiger ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Structural Properties ◽  
Unique Gene

Unique Gene-Silencing and Structural Properties of 2′-Fluoro-Modified siRNAs

2011 ◽  
Vol 123 (10) ◽  
pp. 2332-2336 ◽  
Author(s):  
Muthiah Manoharan ◽  
Akin Akinc ◽  
Rajendra K. Pandey ◽  
June Qin ◽  
Philipp Hadwiger ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Structural Properties ◽  
Unique Gene

scholarly journals Human Argonaute2 and Argonaute3 are catalytically activated by different lengths of guide RNA

2020 ◽  
Author(s):  
Mi Seul Park ◽  
GeunYoung Sim ◽  
Audrey C. Kehling ◽  
Kotaro Nakanishi
Keyword(s):  
Gene Silencing ◽  
Fold Increase ◽  
Specific Gene ◽  
Small Interfering Rnas ◽  
Guide Rna ◽  
Guide Rnas ◽  
Argonaute Proteins ◽  
Complementary Rnas ◽  
Standing Question

AbstractRNA interfering is a eukaryote-specific gene silencing by 20∼23 nucleotide (nt) microRNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation. In humans, Argonaute2 (AGO2) has been known as the only slicer while Argonaute3 (AGO3) barely cleaves RNAs. Therefore, the intrinsic slicing activity of AGO3 remains controversial and a long-standing question. Here, we report 14-nt 3′ end-shortened variants of let-7a, miR-27a, and specific miR-17-92 families that make AGO3 an extremely competent slicer by an ∼ 82-fold increase in target cleavage. These RNAs, named cleavage-inducing tiny guide RNAs (cityRNAs), conversely lower the activity of AGO2, demonstrating that AGO2 and AGO3 have different optimum guide lengths for target cleavage. Our study sheds light on the role of tiny guide RNAs.

scholarly journals Human Argonaute2 and Argonaute3 are catalytically activated by different lengths of guide RNA

2020 ◽  
Vol 117 (46) ◽  
pp. 28576-28578
Author(s):  
Mi Seul Park ◽  
GeunYoung Sim ◽  
Audrey C. Kehling ◽  
Kotaro Nakanishi
Keyword(s):  
Gene Silencing ◽  
Specific Gene ◽  
Small Interfering Rnas ◽  
Guide Rna ◽  
Guide Rnas ◽  
Argonaute Proteins ◽  
Rna Interfering ◽  
Complementary Rnas ◽  
Standing Question

RNA interfering is a eukaryote-specific gene silencing by 20∼23-nucleotide (nt) microRNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation. In humans, Argonaute2 (AGO2) has been known as the only slicer while Argonaute3 (AGO3) barely cleaves RNAs. Therefore, the intrinsic slicing activity of AGO3 remains controversial and a long-standing question. Here, we report 14-nt 3′ end-shortened variants of let-7a, miR-27a, and specific miR-17–92 families that make AGO3 an extremely competent slicer, increasing target cleavage up to ∼82-fold in some instances. These RNAs, named cleavage-inducing tiny guide RNAs (cityRNAs), conversely lower the activity of AGO2, demonstrating that AGO2 and AGO3 have different optimum guide lengths for target cleavage. Our study sheds light on the role of tiny guide RNAs.

scholarly journals Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA

Gene Therapy ◽  
2006 ◽  
Vol 14 (1) ◽  
pp. 78-85 ◽  
Author(s):  
A Nakashima ◽  
H Takaku ◽  
H S Shibata ◽  
Y Negishi ◽  
M Takagi ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Guide Rna

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure
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