Method for Large-Scale Isolation of Pancreatic Islets by Tissue Culture of Fetal Rat Pancreas

Diabetes ◽  
1979 ◽  
Vol 28 (8) ◽  
pp. 769-776 ◽  
Author(s):  
C. Hellerstrom ◽  
N. J. Lewis ◽  
H. Borg ◽  
R. Johnson ◽  
N. Freinkel
Keyword(s):  
Tissue Culture ◽  
Pancreatic Islets ◽  
Large Scale ◽  
Rat Pancreas ◽  
Fetal Rat ◽  
Isolation Of Pancreatic Islets

The Role of CCK and Its Analogues in the Organogenesis of the Fetal Rat Pancreas

Pancreas ◽  
1995 ◽  
Vol 10 (3) ◽  
pp. 281-286 ◽  
Author(s):  
Gerhard E. Feurle ◽  
Gerd Hamscher ◽  
Ali E. Firat
Keyword(s):  
Rat Pancreas ◽  
Fetal Rat

Rice Functional Genomics by Transposon Mutagenesis

2002 ◽  
Vol 06 (24) ◽  
pp. 930-935 ◽  
Author(s):  
Chang-deok Han
Keyword(s):  
Tissue Culture ◽  
Transposable Elements ◽  
Functional Genomics ◽  
Large Scale ◽  
Expression Patterns ◽  
Transposon Mutagenesis ◽  
Transposon Tagging ◽  
Genomic Sequences ◽  
Knock Out ◽  
Genetic Cross

Transposable elements are powerful mutagens. Along with genomic sequences, knock-out phenotypes and expression patterns are important information to elucidate the function of genes. In this review, I propose a strategy to develop tranposant lines on a large scale by combining genetic cross and tissue culture of Ac and Ds lines. Based on the facts that Ds tends to be inactive in F2 or later generation and Ds becomes reactivated via tissue culture, a large scale of transposants can be produced by tissue culture of seeds carrying Ac and inactive Ds. In this review, I describe limitations and considerations in operating transposon tagging systems in rice. Also, I discuss the efficiency of our gene trap system and technical procedures to clone Ds flanking DNA.

A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans

Metabolism ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Jack Noel ◽  
Alexander Rabinovitch ◽  
Les Olson ◽  
George Kyriakides ◽  
Joshua Miller ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Large Scale ◽  
High Yield ◽  
Pancreatic Islets Of Langerhans

scholarly journals Long-term effects of glucose on insulin release and glucose oxidation by mouse pancreatic islets maintained in tissue culture

1974 ◽  
Vol 140 (3) ◽  
pp. 377-382 ◽  
Author(s):  
Arne Andersson
Keyword(s):  
Tissue Culture ◽  
B Cells ◽  
Pancreatic Islets ◽  
Insulin Release ◽  
High Glucose ◽  
Glucose Concentration ◽  
Glucose Oxidation ◽  
Extracellular Glucose Concentration ◽  
Extracellular Glucose ◽  
Mouse Pancreatic Islets

Rates of glucose oxidation and insulin release in response to a wide range of glucose concentrations were studied in short-term experiments in isolated mouse pancreatic islets maintained in tissue culture for 6 days at either a physiological glucose concentration (6.7mm) or at a high glucose concentration (28mm). The curves relating glucose oxidation or insulin release to the extracellular glucose concentration obtained with islets cultured in 6.7mm-glucose displayed a sigmoid shape similar to that observed for freshly isolated non-cultured islets. By contrast islets that had been cultured in 28mm-glucose showed a linear relationship between the rate of glucose oxidation and the extracellular glucose concentration up to about 8mm-glucose. The maximal oxidative rate was twice that of the non-cultured islets and the glucose concentration associated with the half-maximal rate considerably decreased. In islets cultured at 28mm-glucose there was only a small increase in the insulin release in response to glucose, probably due to a depletion of stored insulin in those B cells that had been cultured in a high-glucose medium. It is concluded that exposure of B cells for 6 days to a glucose concentration comparable with that found in diabetic individuals causes adaptive metabolic alterations rather than degeneration of these cells.

TISSUE CULTURE REAGENT QUALITY ASSURANCE TESTING IN A LARGE-SCALE VIRUS PRODUCTION LABORATORY

1977 ◽  
pp. 559-570
Author(s):  
CHARLES V. BENTON ◽  
ROGER W. JOHNSON ◽  
ALBERT PERRY ◽  
W.I. JONES ◽  
GEORGE P. SHIBLEY
Keyword(s):  
Tissue Culture ◽  
Quality Assurance ◽  
Large Scale ◽  
Virus Production
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