A search for 4 specific markers linked to Pm3 alleles for resistance to powdery mildew (Blumeria graminis) in rye (Secale cereale)

Abstract To investigate powdery mildew resistance in rye (Secale cereale), 397 inbred lines of winter rye were tested for susceptibility to infection with Blumeria graminis f. sp. secalis. The 50 most tolerant lines and 50 most infected lines were chosen for comparison. They were next tested for the presence of 4 markers linked to 4 alleles for resistance to powdery mildew, identified earlier in common wheat (Triticum aestivum). We found Pm3a only in 3 susceptible genotypes of winter rye, although this marker is linked to the powdery mildew resistance gene in wheat. The other 3 markers linked to Blumeria graminis f. sp. secalis resistance genes (Pm3b, Pm3c, Pm3d) were found in neither resistant nor susceptible rye genotypes.

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Using the 6RLKu Minichromosome of Rye (Secale cereale L.) to Create Wheat-Rye 6D/6RLKu Small Segment Translocation Lines with Powdery Mildew Resistance

International Journal of Molecular Sciences ◽

10.3390/ijms19123933 ◽

2018 ◽

Vol 19 (12) ◽

pp. 3933 ◽

Cited By ~ 8

Author(s):

Haimei Du ◽

Zongxiang Tang ◽

Qiong Duan ◽

Shuyao Tang ◽

Shulan Fu

Keyword(s):

Powdery Mildew ◽

Resistance Gene ◽

Secale Cereale ◽

Powdery Mildew Resistance ◽

Translocation Chromosome ◽

Translocation Line ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance ◽

Small Segment ◽

Secale Cereale L

Long arms of rye (Secale cereale L.) chromosome 6 (6RL) carry powdery mildew resistance genes. However, these sources of resistance have not yet been successfully used in commercial wheat cultivars. The development of small segment translocation chromosomes carrying resistance may result in lines carrying the 6R chromosome becoming more commercially acceptable. However, no wheat-rye 6RL small segment translocation line with powdery mildew resistance has been reported. In this study, a wheat-rye 6RLKu minichromosome addition line with powdery mildew resistance was identified, and this minichromosome was derived from the segment between L2.5 and L2.8 of the 6RLKu chromosome arm. Following irradiation, the 6RLKu minichromosome divided into two smaller segments, named 6RLKumi200 and 6RLKumi119, and these fragments participated in the formation of wheat-rye small segment translocation chromosomes 6DS/6RLKumi200 and 6DL/6RLKumi119, respectively. The powdery mildew resistance gene was found to be located on the 6RLKumi119 segment. Sixteen 6RLKumi119-specific markers were developed, and their products were cloned and sequenced. Nucleotide BLAST searches indicated that 14 of the 16 sequences had 91–100% similarity with nine scaffolds derived from 6R chromosome of S. cereale L. Lo7. The small segment translocation chromosome 6DL/6RLKumi119 makes the practical utilization in agriculture of powdery mildew resistance gene on 6RLKu more likely. The nine scaffolds are useful for further studying the structure and function of this small segment.

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Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines

Frontiers in Plant Science ◽

10.3389/fpls.2017.01716 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 6

Author(s):

Qiong Duan ◽

Yang Yang Wang ◽

Ling Qiu ◽

Tian Heng Ren ◽

Zhi Li ◽

...

Keyword(s):

Powdery Mildew ◽

Resistance Gene ◽

Secale Cereale ◽

Powdery Mildew Resistance ◽

Chromosome 4 ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance ◽

Physical Location ◽

Secale Cereale L

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Estimation of winter rye (Secale cereale L.) susceptibility to infection by powdery mildew (Blumeria graminis F. sp. secalis)

Acta Agrobotanica ◽

10.5586/aa.2013.037 ◽

2013 ◽

Vol 66 (3) ◽

pp. 49-54

Author(s):

Henryk Bujak ◽

Andrzej Jurkowski

Keyword(s):

Powdery Mildew ◽

Inbred Lines ◽

Blumeria Graminis ◽

Winter Rye ◽

Point Scale ◽

Crossing Experiments ◽

Homogeneous Groups ◽

Susceptibility To Infection ◽

Secale Cereale L ◽

Powdery Mildew Infection

In cold and wet years, powdery mildew (Blumeria graminis) causes losses in the yield of winter rye (Secale cerale L.) amounting to 20%. In order to control the incidence of this disease and reduce the use of fungicides, it is recommendable to grow winter rye cultivars that are resistant or tolerant to infection by Blumeria graminis. The first step in the programmes of resistance-oriented cultivation of winter rye is the choice of adequate components for crossing. Such components should be characterized by resistance to powdery mildew infection, a trait which would be passed onto the new genotypes to be obtained. The paper discusses the outcome of research aimed at selecting inbred lines of winter rye distinguishable by the lowest susceptibility to infection by Blumeria graminis. The evaluation covered 233 winter rye genotypes which had been inoculated with a field population of the pathogen mentioned. The level of infection was defined in a five-point scale from 0 to 4 (the authors’ own scale). Next, the results were analyzed statistically. The genotypes under study were divided using Duncan’s test into homogeneous groups. Seven such groups were distinguished. The first homogeneous group, of the lowest level of infection, comprised five inbred lines of rye. These lines can serve as components for crossing experiments in programmes of resistance-oriented breeding.

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Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat

Genome ◽

10.1139/g05-016 ◽

2005 ◽

Vol 48 (4) ◽

pp. 585-590 ◽

Cited By ~ 74

Author(s):

Zhendong Zhu ◽

Ronghua Zhou ◽

Xiuying Kong ◽

Yuchen Dong ◽

Jizeng Jia

Keyword(s):

Powdery Mildew ◽

Microsatellite Markers ◽

Resistance Gene ◽

Common Wheat ◽

Resistance Genes ◽

Powdery Mildew Resistance ◽

Wheat Chromosome ◽

The Other ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance

Two dominant powdery mildew resistance genes introduced from Triticum carthlicum accession PS5 to common wheat were identified and tagged using microsatellite markers. The gene designated PmPS5A was placed on wheat chromosome 2AL and linked to the microsatellite marker Xgwm356 at a genetic distance of 10.2 cM. Based on the information of its origin, chromosome location, and reactions to 5 powdery mildew isolates, this gene could be a member of the complex Pm4 locus. The 2nd gene designated PmPS5B was located on wheat chromosome 2BL with 3 microsatellite markers mapping proximally to the gene: Xwmc317 at 1.1 cM; Xgwm111 at 2.2 cM; and Xgwm382 at 4.0 cM; and 1 marker, Xgwm526, mapping distally to the gene at a distance of 18.1 cM. Since this gene showed no linkage to the other 2 known powdery mildew resistance genes on wheat chromosome 2B, Pm6 and Pm26, we believe it is a novel powdery mildew resistance gene and propose to designate this gene as Pm33.Key words: powdery mildew, resistance gene, microsatellite, Triticum carthlicum, wheat.

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