scholarly journals Occurrence and Expression of Toxin Genes in Clostridium Perfringens Isolates from Pigs

2012 ◽  
Vol 56 (4) ◽  
pp. 495-498 ◽  
Author(s):  
Elżbieta Kukier ◽  
Magdalena Goldsztejn ◽  
Tomasz Grenda ◽  
Krzysztof Kwiatek
Keyword(s):  
Clostridium Perfringens ◽  
Type A ◽  
Qualitative Assessment ◽  
Toxin Genes ◽  
Genes Expression

Abstract Clostridium perfringens isolates were obtained from pigs of five porcine farms in Poland. The presence of C. perfringens was detected in 92% of faeces samples and its number ranged from 1.0 x 101 cfu/g to 1.2 x 107 cfu/g. All the isolates belonged to type A and 48.7% of them contained cpb2 gene. The qualitative assessment of toxin genes expression by type A subtype β2 isolates showed expression of cpa gene in 100% of strains and cpb2 gene in 71% of the analysed strains. The isolate from one-day-old piglets demonstrated also the expression of cpa and cpb2 genes.

scholarly journals Clostridium perfringens Type E Animal Enteritis Isolates with Highly Conserved, Silent Enterotoxin Gene Sequences

1998 ◽  
Vol 66 (9) ◽  
pp. 4531-4536 ◽  
Author(s):  
Stephen J. Billington ◽  
Eva U. Wieckowski ◽  
Mahfuzur R. Sarker ◽  
Dawn Bueschel ◽  
J. Glenn Songer ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Horizontal Transfer ◽  
Type A ◽  
Enterotoxin Gene ◽  
Ribosome Binding ◽  
Toxin Genes ◽  
Neonatal Calves ◽  
Hemorrhagic Enteritis ◽  
Episomal Dna ◽  
Genotype E

ABSTRACT Several Clostridium perfringens genotype E isolates, all associated with hemorrhagic enteritis of neonatal calves, were identified by multiplex PCR. These genotype E isolates were demonstrated to express α and ι toxins, but, despite carrying sequences for the gene (cpe) encoding C. perfringens enterotoxin (CPE), were unable to express CPE. These silent cpe sequences were shown to be highly conserved among type E isolates. However, relative to the functionalcpe gene of type A isolates, these silent type Ecpe sequences were found to contain nine nonsense and two frameshift mutations and to lack the initiation codon, promoters, and ribosome binding site. The type E animal enteritis isolates carrying these silent cpe sequences do not appear to be clonally related, and their silent type E cpe sequences are always located, near the ι toxin genes, on episomal DNA. These findings suggest that the highly conserved, silent cpe sequences present in most or all type E isolates may have resulted from the recent horizontal transfer of an episome, which also carries ι toxin genes, to several different type A C. perfringens isolates.

Ulcerative Enteritis-Like Disease Associated With Clostridium Perfringens Type A In Bobwhite Quail (Colinus virginianus)

2008 ◽  
Vol 3 (4) ◽  
pp. e23-e24
Author(s):  
H. L. Shivaprasad ◽  
Francisco Uzal ◽  
Randy Kokka ◽  
Derek J. Fisher ◽  
Bruce A. Mcclane ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Colinus Virginianus ◽  
Type A ◽  
Bobwhite Quail

Immunobiology of Clostridium perfringens Type A: Passive Protection of NMRI Mice

Chemotherapy ◽  
1991 ◽  
Vol 37 (5) ◽  
pp. 318-326
Author(s):  
Walter H. Traub ◽  
Dierk Bauer ◽  
Ursula Wolf
Keyword(s):  
Clostridium Perfringens ◽  
Type A ◽  
Passive Protection ◽  
Nmri Mice

scholarly journals Adhesive properties of an outer structure of Clostridium perfringens type A isolated from piglets with with catarrhal enteritis

1999 ◽  
Vol 30 (3) ◽  
pp. 242-248 ◽  
Author(s):  
Elizabeth Pelosi Teixeira ◽  
Marlene Braide Serafim ◽  
Maria Alice Cruz Höfling ◽  
Aureo T. Yamada ◽  
Antonio Fernando Pestana de Castro
Keyword(s):  
Clostridium Perfringens ◽  
Type A ◽  
Negative Control ◽  
Bacterial Cells ◽  
Intestinal Epithelial ◽  
Adhesive Properties ◽  
Ileal Loop ◽  
Fibrillar Material ◽  
Gram Staining ◽  
Transmission Electron

One strain (S32) of Clostridium perfringens type A was isolated from a case of catarrhal enteritis of piglets. This strain was able to adhere to HeLa cells showing an adherence index (AI) of 25.15 ± 1.26 (mean ± 1 standard error of the mean). Treatment of the bacterial cells with trypsin (0.25mg/ml) decreased in 70%-80% the AI and metaperiodate (10mg/ml) abolished completely the adherence, suggesting that the structure responsible for this phenomenon was probably a glycoprotein. Heating of bacterial suspensions (100ºC/5 min) before carrying out the adhesion test decreased the AI rendering it equal to the negative controls. Rabbit homologous S32 antiserum inhibited the adherence up to dilutions of 1: 640, at least. The piglet ileal loop assay, carried out with strains S32 and Jab-1 (negative control) demonstrated that the strain S32 was able to adhere to the intestinal epithelial cells when examined after Gram staining. Transmission electron microcopy (TEM) demonstrated that S32 strain displayed a loose fibrillar material not seen with Jab-1. Stabilization of the bacterial cells with homologous antiserum of strain S32, followed by staining with rhuteniun red, revealed loose long fibrillar material on the outer surface of the cells, that sometimes could be seen spreading out from the cells and linking bacterial cells. The question whether this structure might be an adhesin for this strain of Cl. perfringes type A, perhaps playing a role in the pathogenesis of the catarrhal enteritis of piglets, is dependent on further studies.

Virulence of Clinical and Fecal Isolates of Clostridium perfringens Type A for Outbred NMRI Mice

Chemotherapy ◽  
1991 ◽  
Vol 37 (6) ◽  
pp. 426-435 ◽  
Author(s):  
Walter H. Traub ◽  
Dierk Bauer ◽  
Ursula Wolf
Keyword(s):  
Clostridium Perfringens ◽  
Type A ◽  
Nmri Mice
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