Usage of RNAi-based trigger gene silencing to identify pathogenicity factors of Entamoeba histolytica involved in amoebic liver abscess formation

2017 ◽  
Author(s):  
Sarah Corinna Lender
Keyword(s):  
Gene Silencing ◽  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Abscess Formation ◽  
Amoebic Liver Abscess ◽  
Pathogenicity Factors

Entamoeba histolytica: induction of cyclooxygenase-2 expression during amoebic liver abscess formation in hamsters (Mesocricetus auratus)

2004 ◽  
Vol 106 (3-4) ◽  
pp. 119-125 ◽  
Author(s):  
B. Sánchez-Ramı́rez ◽  
M. Ramı́rez-Gil ◽  
I. Vázquez-Moctezuma ◽  
E. Ramos-Martı́nez ◽  
P. Talamás-Rohana
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Mesocricetus Auratus ◽  
Cyclooxygenase 2 ◽  
Abscess Formation ◽  
Amoebic Liver Abscess

Entamoeba histolytica: Inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites

2006 ◽  
Vol 114 (3) ◽  
pp. 154-159 ◽  
Author(s):  
A. Gutiérrez-Alarcón ◽  
M. Moguel-Torres ◽  
O. Mata-Leyva ◽  
G. Cuellar-Nevárez ◽  
T. Siqueiros-Cendón ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Inflammatory Process ◽  
Cyclooxygenase 2 ◽  
Abscess Formation ◽  
Amoebic Liver Abscess

scholarly journals Overexpression of Specific Cysteine Peptidases Confers Pathogenicity to a Nonpathogenic Entamoeba histolytica Clone

mBio ◽  
2013 ◽  
Vol 4 (2) ◽  
Author(s):  
Jenny Matthiesen ◽  
Ann-Katrein Bär ◽  
Anne-Kathrin Bartels ◽  
Dennis Marien ◽  
Susann Ofori ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Clinical Symptoms ◽  
Axenic Culture ◽  
Protozoan Parasite ◽  
Culture Conditions ◽  
Amoebic Liver Abscess ◽  
Content Type ◽  
Pathogenicity Factors ◽  
Cysteine Peptidases

ABSTRACTCysteine peptidases (CPs) ofEntamoeba histolyticaare considered to be important pathogenicity factors. Previous studies have found that under standard axenic culture conditions, only four (ehcp-a1,ehcp-a2,ehcp-a5, andehcp-a7) out of 35 papain-likeehcpgenes present in theE. histolyticagenome are expressed at high levels. Little is known about the expression of CPs inE. histolyticaduring amoebic liver abscess (ALA) formation. In the current study, a quantitative real-time PCR assay was developed to determine the expression of the variousehcpgenes during ALA formation in animal models. Increased expression of fourehcpgenes (ehcp-a3,-a4,-a10, and-c13) was detected in the gerbil and mouse models. Increased expression of another threeehcpgenes (ehcp-a5,-a6, and-a7) was detected in the mouse model only, and two otherehcpgenes (ehcp-b8and-b9) showed increased expression in the gerbil model only. Trophozoites of the nonpathogenicE. histolyticaHM-1:IMSS clone A1, which was unable to induce ALAs, were transfected with vectors enabling overexpression of those CPs that are expressed at high levels under culture conditions or during ALA formation. Interestingly, overexpression ofehcp-b8,-b9, and-c13restored the pathogenic phenotype of the nonpathogenic clone A1 whereas overexpression of various other peptidase genes had no effect on the pathogenicity of this clone.IMPORTANCEEntamoeba histolyticais a widespread and clinically important protozoan parasite. It normally exists in the human intestine without causing clinical symptoms but can invade the intestinal mucosa, which causes serious intestinal (amoebic colitis) and extraintestinal (amoebic liver abscess [ALA]) diseases. The identification of factors responsible for the invasion of the parasite and disease formation is a major topic in the field. Here, we investigate the roles of different papain-like cysteine peptidases (CPs) as pathogenicity factors. We show that the expression of some of the peptidases that are normally expressed at low levels increases during ALA formation. Furthermore, nonpathogenic amoebae can be transformed to pathogenic amoebae, simply by specific overexpression of some of these CPs. Our findings reinforce the importance of CPs as pathogenicity factors ofE. histolytica.

scholarly journals Entamoeba histolytica acetyl–CoA synthetase: biomarker of acute amoebic liver abscess

2014 ◽  
Vol 4 (6) ◽  
pp. 446-450
Author(s):  
Lim Boon Huat ◽  
Alfonso Olivos Garcia ◽  
Tan Zi Ning ◽  
Wong Weng Kin ◽  
Rahmah Noordin ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Acetyl Coa

scholarly journals Trigger‐induced RNAi gene silencing to identify pathogenicity factors of Entamoeba histolytica

2018 ◽  
Vol 33 (2) ◽  
pp. 1658-1668 ◽  
Author(s):  
Jenny Matthiesen ◽  
Corinna Lender ◽  
Anne Haferkorn ◽  
Helena Fehling ◽  
Martin Meyer ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Entamoeba Histolytica ◽  
Pathogenicity Factors

Linearolactone and Kaempferol Disrupt the Actin Cytoskeleton in Entamoeba histolytica: Inhibition of Amoebic Liver Abscess Development

2020 ◽  
Vol 83 (12) ◽  
pp. 3671-3680
Author(s):  
José Antonio Velázquez-Domínguez ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Fernando Calzada ◽  
Luis Varela-Rodríguez ◽  
Diana L. Pichardo-Hernández ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess

Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess

2013 ◽  
Vol 134 (4) ◽  
pp. 504-510 ◽  
Author(s):  
Nurulhasanah Othman ◽  
Zeehaida Mohamed ◽  
Maya Mazuwin Yahya ◽  
Voon Meng Leow ◽  
Boon Huat Lim ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Antigenic Protein
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