scholarly journals Intracytoplasmic Sperm Injection (ICSI) in B6D2F1 and CB6F1 Strains Mice Using Cauda Epididymal Spermatozoa

Experimed ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 179-183
Author(s):  
Ali Taşkın ◽  
Ahmet Kocabay ◽  
Nilhan Çoşkun
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Epididymal Spermatozoa

scholarly journals Correlation between the motility of frozen-thawed epididymal spermatozoa and the outcome of intracytoplasmic sperm injection

1999 ◽  
Vol 22 (5) ◽  
pp. 324-328 ◽  
Author(s):  
Shibahara ◽  
Hamada ◽  
Hasegawa ◽  
Toji ◽  
Shigeta ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Epididymal Spermatozoa

79 DEVELOPMENT TO TERM OF RAT ZYGOTES DERIVED FROM CRYOPRESERVED MATURE OOCYTES AND SPERM THROUGH INTRACYTOPLASMIC SPERM INJECTION

2008 ◽  
Vol 20 (1) ◽  
pp. 120
Author(s):  
N. Kashiwazaki ◽  
D. Sano ◽  
Y. Seita ◽  
S. Sugio ◽  
C. Suzukamo ◽  
...  
Keyword(s):  
Germ Cells ◽  
Intracytoplasmic Sperm Injection ◽  
Wistar Rats ◽  
Room Temperature ◽  
Developmental Competence ◽  
Key Technology ◽  
Experimental Animals ◽  
Epididymal Spermatozoa ◽  
Vitrification Solution ◽  
Physiological Research

The rat, as well as the mouse, is one of the most valuable experimental animals for biomedical and physiological research. There are numerous valuable mutant rats including transgenetic strains. Cryopreservation of rat oocytes and sperm as haploid germ cells is a key technology for banking the genetic resources efficiently. The aim of the present study was to examine survival of vitrified/warmed oocytes and developmental competence of resultant zygotes in the rat. Rats used in the present study were all Wistar rats. Epididymal spermatozoa were frozen as described previously (Seita et al. 2005 Reprod. Fertil. Dev. 18, 256). After thawing, spermatozoa were sonicated to obtain sperm heads for intracytoplasmic sperm injection (ICSI). Oocytes were collected from immature females superovulated with eCG and hCG. Oocytes were equilibrated in 7.5% (v/v) ethylene glycol (EG) + 7.5% (v/v) dimethylsulfoxide (DMSO) + 20% (v/v) FCS in PB1 for 5 min and then transferred into 15.0% EG (v/v) + 15.0% DMSO (v/v) + 20% FCS + 0.5 m sucrose in PB1 (vitrification solution) for 1 min at room temperature (22–24�C). During exposure to the vitrification solution, oocytes were loaded on a Cryotop� (Kitazato Supply Co., Tokyo, Japan). At warming, the film of Cryotop was directly immersed into PB1 containing 0.5 m sucrose and 20% FCS at 37.5�C. The warmed oocytes were washed three times and put into a HEPES-buffered (22 mm) modified R1ECM (310 mOsm) medium. The sperm heads were microinjected intracytoplasmically into the warmed oocytes. Then, presumptive zygotes were transferred surgically into the oviducts of recipient females (Day 0), and Caesarean section of the recipients was performed on Day 22. After vitrification and warming, 245 of 275 (88%) oocytes survived morphologically, 240 of the warmed oocytes were injected, and 156 oocytes (65%) were morphologically normal after the injection. To confirm development to term of zygotes derived from cryopreserved oocytes and sperm, 143 injected oocytes were transferred to 9 recipients, resulting in 3 pregnancies and the generation of one live pup. The results indicate that rat zygotes derived from cryopreserved oocytes and sperm through ICSI can develop to term, and full developmental competence can be preserved in rat oocytes after cryopreservation.

Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa

2001 ◽  
Vol 56 (5) ◽  
pp. 955-967 ◽  
Author(s):  
L Bogliolo ◽  
G Leoni ◽  
S Ledda ◽  
S Naitana ◽  
M Zedda ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Domestic Cats ◽  
Epididymal Spermatozoa

scholarly journals In Vitro Fertilization Outcome After Intracytoplasmic Sperm Injection with Fresh and with Frozen-Thawed Epididymal Spermatozoa

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Hilma Putri Lubis
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Oocyte Retrieval ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Sperm Aspiration ◽  
Significant Difference ◽  
Epididymal Spermatozoa

<p><strong>Introduction</strong></p><p>Testicular epididymal sperm aspiration (TESA) is one of the method  to retrieve sperm from the testes in men with azoospermia. The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with  oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa.</p><p><strong>Material &amp; Methods</strong></p><p>A retrospective comparative analysis of  patients who underwent fresh TESA and frozen-thawed TESA in ICSI-ET cycles from January 2012 to December 2014 in Halim Fertility Center was done. Fresh testicular epididymal sperm aspiration (fresh TESA) was performed on the same day with oocyte retrieval in 28 cycles and the frozen-thawed testicular epididymal sperm aspiration (frozen-thawed TESA) was used in 30 cycles.  </p><p><strong>Results</strong></p><p>The two groups were comparable in terms of the ages of male and female patients, etiology of infertility and duration of infertility. Fertilization rates in fresh TESA group were 53,5% and in frozen-thawed TESA group, fertilization rates were 50%. There was no statistically significant difference between the groups. Clinical pregnancy rates in fresh TESA group were 35,7%  and in frozen-thawed TESA group, clinical pregnancy rates were 26,7% and statistically there was no significant difference between the groups.</p><p><strong>Conclusion</strong></p>There is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA .

Fertilization and early embryology: Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen—thawed epididymal spermatozoa

1995 ◽  
Vol 10 (4) ◽  
pp. 903-906 ◽  
Author(s):  
Paul Devroey ◽  
Sherman Silber ◽  
Zsolt Nagy ◽  
Jiaen Liu ◽  
Herman Tournaye ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Epididymal Spermatozoa

Frozen-thawed epididymal spermatozoa for intracytoplasmic sperm injection

1997 ◽  
Vol 67 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Carol A. Holden ◽  
Giuliana F. Fuscaldo ◽  
Peter Jackson ◽  
Alex Cato ◽  
Graeme J. Southwick ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Epididymal Spermatozoa
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