In vitro study of complexing properties of high-molecular water-soluble polymer in relation to model protein

2017 ◽  
Vol 43 (1) ◽  
pp. 57-60
Author(s):  
V. V. Bydanova
Keyword(s):  
In Vitro Study ◽  
Water Soluble ◽  
Vitro Study ◽  
Molecular Water ◽  
Soluble Polymer ◽  
Water Soluble Polymer ◽  
Model Protein ◽  
Complexing Properties

Investigation of the Harmlessness of a High-Molecular Water-Soluble Polymer in Laboratory Animals

2021 ◽  
pp. 570-579
Author(s):  
Natalia Grudina ◽  
Vera Bydanova ◽  
Olga Izmestyeva ◽  
Nikolai Grudin
Keyword(s):  
Water Soluble ◽  
Laboratory Animals ◽  
Molecular Water ◽  
Soluble Polymer ◽  
Water Soluble Polymer

Study of toxic properties of high-molecular water-soluble polymer

2018 ◽  
pp. 56-57
Author(s):  
N. Grudina ◽  
◽  
V. Bydanova ◽  
N. Grudin ◽  
◽  
...  
Keyword(s):  
Water Soluble ◽  
Molecular Water ◽  
Soluble Polymer ◽  
Water Soluble Polymer

scholarly journals Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

2017 ◽  
Vol 25 (4) ◽  
pp. 196-206 ◽  
Author(s):  
Anton Oertl ◽  
Emmanouil Antonopoulos ◽  
Seruni U. Freisleben ◽  
Hans-Joachim Freisleben
Keyword(s):  
Colon Carcinoma ◽  
High Stability ◽  
In Vitro Study ◽  
Low Ph ◽  
Carcinoma Cells ◽  
Water Soluble ◽  
Vitro Study ◽  
Colon Carcinoma Cells ◽  
Diethylaminoethyl Cellulose

Background: Archaeal membranes have phytanyl ether lipids instead of common fatty acid-glycerol esters in bacterial and eukaryotic cells. Sulfolobus and Thermoplasma species have unique membrane-spanning tetraether lipids (TEL), which form stable liposomes. Recently, we cultured Thermoplasma species from the Indonesian volcano Tangkuban Perahu and isolated TEL. The purpose of this in vitro study is to investigate the transfer of fluorescent dye from stable TEL liposomes to cultured colon carcinoma cells.Methods: TEL was extracted from cultured cells with chloroform-methanol (1:1), then it was fractionated and purified via diethylaminoethyl-cellulose-acetate columns and activated charcoal for the formation of stable liposomes. For the fluorescence exchange assay, TEL liposomes were loaded with water-soluble carboxyfluorescein (CF). Staining experiments were conducted with various cell cultures, and T84 colon carcinoma cells were chosen for the main experiments. Liposome stability was tested by light scattering and electron microscopic size determinations as well as by unspecific CF release at low pH (6.0–7.4) and increased temperature  (4–50°C/70°C).Results: TEL liposomes exhibit high stability and extremely low proton permeability at low pH. CF staining of cultured T84 colon carcinoma cells appeares more intensive from TEL liposomes than from dipalmitoylphosphatidylcholine liposomes.Conclusion: The results of this in vitro study demonstrate CF staining of colon carcinoma cells and high stability of TEL liposomes at low pH, matching the condition in the gastro-intestinal (GI) route and in the urogentital (UG) tract. For this reason, in vivo studies on liposomal fluorescent photosensitizers for topical application of photodynamic cancer therapy in the GI and UG tracts should be carried out.

In Vitro Study of DNA Interaction with a Water-Soluble Dinitrogen Schiff Base

2009 ◽  
Vol 28 (11) ◽  
pp. 589-596 ◽  
Author(s):  
Nahid Shahabadi ◽  
Soheila Kashanian ◽  
Farivash Darabi
Keyword(s):  
Schiff Base ◽  
In Vitro Study ◽  
Dna Interaction ◽  
Water Soluble ◽  
Vitro Study

Regiospecific vs. non regiospecific click azide-alkyne polymerization: In vitro study of water-soluble antibacterial poly(amide aminotriazole)s

2021 ◽  
pp. 112113
Author(s):  
Cristian Rangel-Núñez ◽  
Cristina Ramírez-Trujillo ◽  
Khalid Hakkou ◽  
Adrián Suárez-Cruz ◽  
Inmaculada Molina-Pinilla ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Water Soluble ◽  
Vitro Study

scholarly journals Antibacterial Activity of Haslea ostrearia Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study)

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 30
Author(s):  
Ikfa Permatasari ◽  
Fiddy Semba Prasetiya ◽  
Sulastri Arsad ◽  
Mochamad Untung Kurnia Agung ◽  
Jean-Luc Mouget
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
In Vitro Study ◽  
Water Soluble ◽  
Vitro Study ◽  
Inhibitory Zone ◽  
Haslea Ostrearia

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic

Sign in / Sign up

Export Citation Format

Share Document