Studies on Intrinsic Factor and Pernicious Anemia: III. Intrinsic Factor Activity and B12-Binding Power of Electrophoretic Fractions of Intrinsic Factor Concentrates

Abstract Fractionation of desiccated defatted hog stomach through the use of isoelectric and ammonium sulfate precipitation technics, and guided by assays in patients with pernicious anemia in relapse given daily oral doses of 5 µg. of vitamin B12, has led to the concentration of intrinsic factor activity in an amount of protein equivalent to 0.03 per cent of the original weight of crude material. The electrophoretic pattern of this active fraction indicated a lack of homogeneity. The vitamin B12-binding activities of three fractions obtained by precipitation with ammonium sulfate were investigated by microbial inhibition and dialysis methods. There was no correlation between the ability of the fractions to bind vitamin B12 and to exert intrinsic factor activity. Among these fractions, that with the highest intrinsic factor activity in patients with pernicious anemia in relapse bound the least amount of vitamin B12.

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Quantitative Assay of Intrinsic Factor Activity by Urinary Excretion of Radioactive Vitamin B12

Blood ◽

10.1182/blood.v13.6.582.582 ◽

1958 ◽

Vol 13 (6) ◽

pp. 582-588 ◽

Cited By ~ 23

Author(s):

LEON ELLENBOGEN ◽

WILLIAM L. WILLIAMS

Keyword(s):

Oral Dose ◽

Urinary Excretion ◽

Vitamin B12 ◽

Pernicious Anemia ◽

Intrinsic Factor ◽

Quantitative Assay ◽

Factor Activity ◽

Anemia Patient

Abstract A technic for assaying consecutively up to 18 intrinsic factor preparations with one pernicious anemia patient is described. The use of repeated flushing doses appeared to have little effect on the respsonse in the urinary excretion test; nine patients were given up to 38 flushing doses of 1 mg. of nonradioactive B12 over a period of 44 days and showed essentially the same response to a standard intrinsic factor preparation at the beginning and end of this period. The excretion of vitamin B12 in the test increased with increasing amounts of intrinsic factor in each of four different preparations, indicating that large amounts of intrinsic factor did not inhibit absorption. There was no relationship between the urinary excretion values obtained with radioactive B12 alone and the values obtained with radioactive B12 plus standard intrinsic factor in a series of tests on 127 pernicious anemia patients. It appears that total rather than net excretion values are to be preferred in evaluating preparations. Since it has been shown that pernicious anemia patients given intrinsic factor absorb more than 0.5 µg. of radioactive vitamin B12, an oral dose of 2.0 µg. of B12 which allows an absorption of more than 0.5 µg. is preferred in order to extend the range of intrinsic factor test doses that can be assayed.

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Pernicious Anemia without Gastric Atrophy and in the Presence of Free Hydrochloric Acid

Blood ◽

10.1182/blood.v12.5.461.461 ◽

1957 ◽

Vol 12 (5) ◽

pp. 461-468 ◽

Cited By ~ 25

Author(s):

J. N. HARRIS-JONES ◽

H. T. SWAN ◽

G. R. TUDHOPE

Keyword(s):

Hydrochloric Acid ◽

Gastric Mucosa ◽

Vitamin B12 ◽

Pernicious Anemia ◽

Gastric Juice ◽

Intrinsic Factor ◽

Gastric Atrophy ◽

Free Hydrochloric Acid ◽

Normal Gastric Mucosa ◽

Factor Activity

Abstract A case is described of Addisonian pernicious anemia in a girl aged 16 years, associated with a normal gastric mucosa and free hydrochloric acid in the stomach. Partial, but significant deficiency of intrinsic factor activity was demonstrated in her gastric juice by the use of Co56-labeled vitamin B12.

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An in vitro assay for intrinsic factor activity of porcine mucosal concentrates

Analytical Biochemistry ◽

10.1016/0003-2697(64)90219-2 ◽

1964 ◽

Vol 7 (2) ◽

pp. 129-134 ◽

Cited By ~ 14

Author(s):

Hans J. Hansen ◽

O.Neal Miller

Keyword(s):

Intrinsic Factor ◽

In Vitro Assay ◽

Factor Activity ◽

Vitro Assay

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Intrinsic Factor Activity of Normal Serum after Intravenously Administered Heparin

Fünfter Kongress der Europäischen Gesellschaft für Hämatologie ◽

10.1007/978-3-662-25760-9_74 ◽

1956 ◽

pp. 288-289

Author(s):

Ragnar Berlin

Keyword(s):

Intrinsic Factor ◽

Normal Serum ◽

Factor Activity

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Chronic Atrophic Gastritis with Negative Intrinsic Factor and Parietal Cell Antibody Presenting as a Severe Hemolytic Anemia

Case Reports in Hematology ◽

10.1155/2020/8697493 ◽

2020 ◽

Vol 2020 ◽

pp. 1-4

Author(s):

G. F. Cittolin-Santos ◽

S. Khalil ◽

J. K. Bakos ◽

K. Baker

Keyword(s):

Vitamin B12 ◽

Atrophic Gastritis ◽

Pernicious Anemia ◽

Hemolytic Anemia ◽

Parietal Cell ◽

Intrinsic Factor ◽

Peripheral Blood Smear ◽

Chronic Atrophic Gastritis ◽

Cell Antibody ◽

Parietal Cell Antibody

A 28-year-old Caucasian male with Hashimoto’s disease and vitiligo presented with two weeks of dizziness on exertion following pharyngitis which was treated with prednisone 40 mg by mouth once a day for five days. Initial workup revealed anemia, elevated lactate dehydrogenase (LDH), and low haptoglobin. He underwent workup for causes of hemolytic anemia which was remarkable for a peripheral blood smear with hypersegmented neutrophils and low vitamin B12 levels concerning for pernicious anemia. Parietal cell and intrinsic factor antibodies were negative, and he then underwent an esophagogastroduodenoscopy with biopsy. The biopsy was negative for Helicobacter pylori, and the immunohistochemical stains were suggestive of chronic atrophic gastritis. He was started on vitamin B12 1,000 mcg intramuscular injections daily. His hemoglobin, LDH, and haptoglobin normalized. Given the absence of the parietal cell antibody and intrinsic factor antibody, this is a rare case of seronegative pernicious anemia.

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