The Proportions of Hemoglobin Types Induced in Mouse Erythroleukemia Cells Vary with the Inducer or Combination of Inducers, The Inducer Concentration and the Time of Induction

Hemoglobin ◽  
1985 ◽  
Vol 9 (6) ◽  
pp. 577-596 ◽  
Author(s):  
William Scher ◽  
Barbara M. Scher ◽  
Nella Hellinger ◽  
Samuel Waxman
Keyword(s):  
Inducer Concentration ◽  
Erythroleukemia Cells ◽  
Mouse Erythroleukemia

Rapid induction of polyadenylated H1 histone mRNAs in mouse erythroleukemia cells is regulated by c-myc

1989 ◽  
Vol 9 (6) ◽  
pp. 2332-2340
Author(s):  
G H Cheng ◽  
A I Skoultchi
Keyword(s):  
Terminal Differentiation ◽  
Histone Genes ◽  
H1 Histone ◽  
Rapid Induction ◽  
Histone Mrnas ◽  
Cellular Genes ◽  
Erythroleukemia Cells ◽  
Multistep Process ◽  
Murine Erythroleukemia ◽  
Mouse Erythroleukemia

Chemically induced differentiation of murine erythroleukemia cells is a multistep process involving a precommitment period in which exposure to inducer leads to cells that are irreversibly committed to terminal differentiation. Certain changes in the expression of cellular proto-oncogenes are an important feature of the precommitment phase. We have identified two H1 histone genes that are rapidly induced during this period. Unlike most histone genes, these two H1 genes encode polyadenylated mRNAs with long 3' untranslated regions. To investigate the relationship between induction of the H1 mRNAs and changes in proto-oncogene expression, we studied two independent series of mouse erythroleukemia cell lines that are inhibited from differentiating because of deregulated expression of transfected copies of c-myc or c-myb. The results showed that induction of the H1 mRNAs was negatively regulated by c-myc. The two H1 histone genes are among the first examples of specific cellular genes that are regulated by c-myc. The timing of their induction suggests that they may play an important role in achieving commitment to terminal differentiation.

Induction of mouse erythroleukemia cells by microinjection of inducing compound

1981 ◽  
Vol 131 (1) ◽  
pp. 73-77 ◽  
Author(s):  
E DIACUMAKOS ◽  
L KILLOS ◽  
L LEE ◽  
W ANDERSON
Keyword(s):  
Erythroleukemia Cells ◽  
Mouse Erythroleukemia

scholarly journals Globin synthesis in mouse erythroleukemia cells in vitro: a switch in beta chains due to inducing agent

Blood ◽  
1977 ◽  
Vol 50 (5) ◽  
pp. 867-876 ◽  
Author(s):  
BP Alter ◽  
SC Goff
Keyword(s):  
Protein Synthesis ◽  
Butyric Acid ◽  
Cell System ◽  
Globin Chain ◽  
Erythroleukemia Cells ◽  
Friend Cells ◽  
Erythroleukemia Cell ◽  
Globin Synthesis ◽  
Mouse Erythroleukemia

Abstract Friend erythroleukemia cells, originally derived from DBA/2 mice, differentiate when cultured with inducing agents. Studies of the different effects of inducing agents on clone 745 have revealed that both dimethyl sulfoxide (DMSO) and hemin produce benzidine-positive cells. Butyric acid produced mature but benzidine-negative cells in this clone. All agents induced globin synthesis above the 0.1% of protein synthesis found in uninduced cells. DMSO induction stimulated globin synthesis 9%, hemin 2%, and butyric acid 3%. Total beta/alpha ratios were approximately unity with all agents. Although the inducing agents all stimulated total globin synthesis in Friend cells, the relative rates of synthesis of the two mouse beta chains were affected differently by the various agents. Hemin markedly increased the proportion of beta minor. For example, DBA/2 mouse reticulocytes synthesized 20% beta minor and 80% beta major. DMSO induction of clone 745 caused 20%-33% synthesis of beta minor. In contrast, hemin increased the proportion of beta minor to 64%-69%. Thus the Friend erythroleukemia cell system provides an in vitro approach to the study of the regulation of globin-chain switching.

Sign in / Sign up

Export Citation Format

Share Document