Irradiation-sterilization of rat bone matrix gelatin

1988 ◽  
Vol 59 (2) ◽  
pp. 165-167 ◽  
Author(s):  
Nikolaus Schwarz ◽  
Heinz Redl ◽  
Anna Schiesser ◽  
Gunther Schlag ◽  
Martin Thurnher ◽  
...  
Keyword(s):  
2003 ◽  
Vol 51 (1) ◽  
pp. 61-67 ◽  
Author(s):  
O. Laboux ◽  
L-G. Ste-Marie ◽  
F.H. Glorieux ◽  
A. Nanci

Methylmethacrylate (MMA) embedding of undecalcified bone is routinely employed for histomorphometric analyses. Although MMA-embedded bone has been used for immunolabeling at the light microscopic level after removal of the resin, there are no such reports for electron microscopy. The aim of the present study was to determine whether MMA embedding can be used for ultrastructural immunolabeling and how it compares to LR White (LRW), an acrylic resin frequently used for immunocytochemistry of bone. Rat tibiae were fixed by vascular perfusion with aldehyde and embedded either in MMA or LRW resin. Thin sections were processed for postembedding protein A-gold immunolabeling with antibodies to rat bone sialoprotein (BSP) and osteopontin (OPN). The density of gold particles over bone was quantified. The density and distribution of immunolabeling for BSP and OPN respectively, were comparable between MMA and LRW. These results indicate that MMA performs as well as LRW for the ultrastructural immunolabeling of noncollagenous bone matrix proteins.


1989 ◽  
Vol 60 (6) ◽  
pp. 693-695 ◽  
Author(s):  
Nikolaus Schwarz ◽  
Hans P. Dinges ◽  
Anna Schiesser ◽  
Heinz Redl ◽  
Günther Schlag
Keyword(s):  
Dog Bone ◽  

1984 ◽  
Vol &NA; (185) ◽  
pp. 266???269
Author(s):  
F. C. HENRIK ◽  
OLLE S. NILSSON ◽  
HANS TORNKVTST ◽  
Tom C. Lindholm ◽  
T. SAM
Keyword(s):  

1992 ◽  
Vol 40 (8) ◽  
pp. 1079-1088 ◽  
Author(s):  
T Ikeda ◽  
S Nomura ◽  
A Yamaguchi ◽  
T Suda ◽  
S Yoshiki

2016 ◽  
Vol 77 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Sebahattin Kandal ◽  
Selahattin Özmen ◽  
Safak Uygur ◽  
Munci Yagci ◽  
Handan Kayhan ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document