scholarly journals Comparison of whole genome sequencing to restriction endonuclease analysis and gel diffusion precipitin test of Pasteurella multocida

2016 ◽  
Author(s):  
Karen Jeanne Lecount
Keyword(s):  
Whole Genome Sequencing ◽  
Restriction Endonuclease ◽  
Genome Sequencing ◽  
Pasteurella Multocida ◽  
Restriction Endonuclease Analysis ◽  
Whole Genome ◽  
Precipitin Test ◽  
Gel Diffusion ◽  
Endonuclease Analysis

scholarly journals Comparison of whole genome sequencing to restriction endonuclease analysis and gel diffusion precipitin-based serotyping of Pasteurella multocida

2017 ◽  
Vol 30 (1) ◽  
pp. 42-55 ◽  
Author(s):  
Karen J. LeCount ◽  
Linda K. Schlater ◽  
Tod Stuber ◽  
Suelee Robbe Austerman ◽  
Timothy S. Frana ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Restriction Endonuclease ◽  
Genome Sequencing ◽  
Pasteurella Multocida ◽  
Restriction Endonuclease Analysis ◽  
The United States ◽  
Snp Analysis ◽  
Whole Genome ◽  
Gel Diffusion ◽  
Endonuclease Analysis

The gel diffusion precipitin test (GDPT) and restriction endonuclease analysis (REA) have commonly been used in the serotyping and genotyping of Pasteurella multocida. Whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis has become the gold standard for other organisms, offering higher resolution than previously available methods. We compared WGS to REA and GDPT on 163 isolates of P. multocida to determine if WGS produced more precise results. The isolates used represented the 16 reference serovars, isolates with REA profiles matching an attenuated fowl cholera vaccine strain, and isolates from 10 different animal species. Isolates originated from across the United States and from Chile. Identical REA profiles clustered together in the phylogenetic tree. REA profiles that differed by only a few bands had fewer SNP differences than REA profiles with more differences, as expected. The GDPT results were diverse but it was common to see a single serovar show up repeatedly within clusters. Several errors were found when examining the REA profiles. WGS was able to confirm these errors and compensate for the subjectivity in analysis of REA. Also, results of WGS and SNP analysis correlated more closely with the epidemiologic data than GDPT. In silico results were also compared to a lipopolysaccharide rapid multiplex PCR test. From the data produced in our study, WGS and SNP analysis was superior to REA and GDPT and highlighted some of the issues with the older tests.

scholarly journals Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

2011 ◽  
Vol 55 (5) ◽  
pp. 2475-2477 ◽  
Author(s):  
Kristina Kadlec ◽  
Geovana Brenner Michael ◽  
Michael T. Sweeney ◽  
Elzbieta Brzuszkiewicz ◽  
Heiko Liesegang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Resistance Genes ◽  
Molecular Basis ◽  
Pasteurella Multocida ◽  
Whole Genome ◽  
Transporter Gene ◽  
Content Type ◽  
Methylase Gene

ABSTRACTThe mechanism of macrolide-triamilide resistance inPasteurella multocidahas been unknown. During whole-genome sequencing of a multiresistant bovineP. multocidaisolate, three new resistance genes, the rRNA methylase geneerm(42), the macrolide transporter genemsr(E), and the macrolide phosphotransferase genemph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)-mph(E)] in macrolide-susceptibleEscherichia coliandP. multocidahosts.

Restriction Endonuclease Analysis of Pasteurella multocida Isolates from Three California Turkey Premises

1992 ◽  
Vol 36 (2) ◽  
pp. 272 ◽  
Author(s):  
K. H. Christiansen ◽  
T. E. Carpenter ◽  
K. P. Snipes ◽  
D. W. Hird ◽  
G. Y. Ghazikhanian
Keyword(s):  
Restriction Endonuclease ◽  
Pasteurella Multocida ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis

Utility of Whole Genome Sequencing in diagnosing complex disorders: lesson from renal tubular disorders

2018 ◽  
Author(s):  
Mark Stevenson ◽  
Alistair T Pagnamenta ◽  
Heather G Mack ◽  
Judith A Savige ◽  
Kate E Lines ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Complex Disorders ◽  
Tubular Disorders ◽  
Renal Tubular Disorders ◽  
Renal Tubular
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