scholarly journals Para-Spinal Ganglion

2020 ◽  
Author(s):  
Keyword(s):  

Author(s):  
Neng-Yu Zhang ◽  
Bruce F. McEwen ◽  
Joachim Frank

Reconstructions of asymmetric objects computed by electron tomography are distorted due to the absence of information, usually in an angular range from 60 to 90°, which produces a “missing wedge” in Fourier space. These distortions often interfere with the interpretation of results and thus limit biological ultrastructural information which can be obtained. We have attempted to use the Method of Projections Onto Convex Sets (POCS) for restoring the missing information. In POCS, use is made of the fact that known constraints such as positivity, spatial boundedness or an upper energy bound define convex sets in function space. Enforcement of such constraints takes place by iterating a sequence of function-space projections, starting from the original reconstruction, onto the convex sets, until a function in the intersection of all sets is found. First applications of this technique in the field of electron microscopy have been promising.To test POCS on experimental data, we have artificially reduced the range of an existing projection set of a selectively stained Golgi apparatus from ±60° to ±50°, and computed the reconstruction from the reduced set (51 projections). The specimen was prepared from a bull frog spinal ganglion as described by Lindsey and Ellisman and imaged in the high-voltage electron microscope.



2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Lohit Velagapudi ◽  
Caio M. Matias ◽  
Christopher Elia ◽  
David Stolzenberg ◽  
Garrett Largoza ◽  
...  




2001 ◽  
Vol 33 (4) ◽  
pp. 342-348 ◽  
Author(s):  
M. Ledda ◽  
L. Barni ◽  
L. Altieri ◽  
E. Pannese


1989 ◽  
Vol 15 (4) ◽  
pp. 467-473 ◽  
Author(s):  
I TOYOSHIMA ◽  
A YAMAMOTO ◽  
O MASAMUNE ◽  
S YAMADA ◽  
M SATAKE


1966 ◽  
Vol 65 (1-3) ◽  
pp. 236-255 ◽  
Author(s):  
Julio Maria Sosa ◽  
Nadir Brum de Zorrilla
Keyword(s):  


1963 ◽  
Vol 25 (3) ◽  
pp. 435-452 ◽  
Author(s):  
S. Shishito ◽  
T. Saito ◽  
K. Imabayashi ◽  
N. Nakano ◽  
Y. Shiraiwa ◽  
...  


1958 ◽  
Vol 4 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Everett Anderson ◽  
V. L. van Breemen

Spinal ganglionic cells of Rana pipiens were studied with light and electron microscopes in normal animals and in animals which had received graded dosages of malononitrile intraperitoneally. After treatment no increase in the intensity of staining was noted in the Nissl substance when spinal ganglion cells were examined with the light microscope. The electron micrographs demonstrated the following in malononitrile-treated animals: 1. The cisternae of the endoplasmic reticulum composing the Nissl bodies appeared to fragment and lose their parallel orientation. 2. The microvesicular components of the Golgi complex appeared to increase in number, and the increase was apparently due to fragmentation of the membrane system of the Golgi complex. 3. The mitochondria enlarged and became pleomorphic, but displayed no alterations of internal structure. The morphological changes may be interpreted as reflections of biochemical alterations.



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