39. Review and Assessment of New Biological Safety Level 3 (BSL-3) Facilities

2004 ◽  
Author(s):  
D. Sassone ◽  
A. Jin ◽  
S. Kawalewsky ◽  
K. Groves
Keyword(s):  
Safety Level ◽  
Biological Safety ◽  
Level 3

SFGR Isolation from Clinical Diagnostic Material: Small volume inoculum v1

2021 ◽  
Author(s):  
Marah Condit ◽  
Cecilia Kato
Keyword(s):  
Small Volume ◽  
Clinical Samples ◽  
Growth Monitoring ◽  
Gram Negative Bacteria ◽  
Safety Level ◽  
Extreme Caution ◽  
Obligate Intracellular ◽  
Biological Safety ◽  
Growth Area ◽  
Level 3

Rickettsiae are small obligate intracellular gram-negative bacteria that are grown in a Biological Safety Level 3 (BSL-3) laboratory setting.This protocol uses antibiotic-free medium, and therefore cultures are susceptible to contamination by undesirable bacteria or fungi.This procedure provides guidance for the inoculation and growth of primary isolates derived from small volumes of clinical samples including blood and blood products in a small volume culture and includes topics of growth monitoring, changing media, freezing primary isolates, and troubleshooting in the event of contamination. This technique utilizes Vero E6 cells growth in small conical culture flasks.These flasks have a growth area of 10cm2 and can accommodate up to 5 mL of media.All techniques are to be performed in a biological safety cabinet (BSC) in a BSL-3 laboratory.Proper training and extreme caution is of the utmost importance when working with live rickettsial cultures.

Corrigendum to “Strengthening the diagnostic capacity to detect Bio Safety Level 3 organisms in unusual respiratory viral outbreaks” [J. Clin. Virol. 45 (2009) 185–190]

2010 ◽  
Vol 47 (2) ◽  
pp. 204 ◽  
Author(s):  
Liselotte van Asten ◽  
Mariken van der Lubben ◽  
Cees van den Wijngaard ◽  
Wilfrid van Pelt ◽  
Robert Verheij ◽  
...  
Keyword(s):  
Safety Level ◽  
Level 3 ◽  
Diagnostic Capacity

scholarly journals Risk Assessment Proposal In HydrauliC Presses With Fuzzy Logic

2021 ◽  
Vol 9 (5) ◽  
pp. 349-368
Author(s):  
Patricia Sluce
Keyword(s):  
At Risk ◽  
Hydraulic Press ◽  
Risk Level ◽  
Safety Level ◽  
Before And After ◽  
Level 3 ◽  
Machine Safety ◽  
Level 1 ◽  
Object Of Study ◽  
Risk Categories

The aim of this study was to evaluate the safety level of industrial machines, in particular hydraulic press. The dissertation used hydraulic presses as the object of study. The research instruments used were machine safety analyzes based on normative items pre-established in ABNT NBR: 12100, possible accidents that these machineries can cause. The results show that hydraulic presses cause many accidents, in some situations dying. Through Annex B of ABNT NBR 14153: 2013, there are 4 risk categories for machinery, the greater the degree of risk, the more unsafe the machine is. The appraiser's experience is very important to analyze the machine and reach the level of risk level before and after the adjustment. Finally, it appears that the machine analyzed in this study was at risk level 3, after analysis and adaptations the same machine was at risk level 1, totally acceptable to maintain the operator's safety level.

scholarly journals Role of the Class III Biological Safety Cabinet in Achieving Biological Safety Level 4 Containment (Chapter 10)

1999 ◽  
Vol 4 (1) ◽  
pp. 33-40 ◽  
Author(s):  
David G. Stuart ◽  
Julia Hilliard ◽  
Richard Henkel ◽  
Jack Kelley ◽  
Jonathan Y. Richmond
Keyword(s):  
Class Iii ◽  
Safety Level ◽  
Biological Safety
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