Resynthesis of new R lines in Brassica napus L.

Identification of male fertility restorer genotypes for rapeseed CMS lines towards hybrid development in spring habit rapeseed (Brassica napus L.) adapted for short day winter season was studied. The experiment was conducted at the experimental farm and laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur during October, 2008 to March, 2011. An exotic CMS-based F1 hybrid of rapeseed was selfed to get F2 generation with a view to resynthesizing restorer line. As a result a restorer line for Nap248A Z1 and Nap248A Z2 cytoplasmic male sterile lines was identified in the F3 generation of the exotic F1 rapeseed hybrid which appears as the first case so far reported as achievement in Bangladesh in this regard. Genetic analysis further revealed fertility restoration for Nap248A Z1 and Nap248A Z2 cytoplasmic male sterility was controlled by a single dominant nuclear gene as a simple genetic phenomenon.Bangladesh J. Agril. Res. 41(3): 529-540, September 2016

Download Full-text

Molecular tagging of the male fertility restorer gene for the 501-8S cytoplasmic male sterility in rapeseed (Brassica napus L.)

Australian Journal of Agricultural Research ◽

10.1071/ar06369 ◽

2007 ◽

Vol 58 (8) ◽

pp. 753

Author(s):

Haohua He ◽

Liang Xu ◽

Xiaosong Peng ◽

Guangsheng Yang ◽

Changlan Zhu ◽

...

Keyword(s):

Brassica Napus ◽

Male Fertility ◽

Mapping Population ◽

Bulked Segregant Analysis ◽

Fertility Restoration ◽

Nuclear Gene ◽

F1 Hybrids ◽

Male Sterile ◽

Brassica Napus L ◽

Rf Gene

The cytoplasmic male sterile (CMS) system has been successfully used to explore heterosis in rapeseed (Brassica napus L.). A newly developed male sterile line (501-8S) was characterised for its male fertility response to temperature and photoperiod, and the inheritance of fertility restoration. Segregation analysis using F1 and F2, BC1, and F3 populations of the crosses between the 501-8S and fertile lines of B. napus revealed that fertility restoration was conferred by a dominant nuclear gene (Rf). The F2 population of the cross 501-8S × Yuyou1 was used as a mapping population to map the Rf gene. A combination of bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) methodology was used to identify putative markers linked to the Rf gene. Twenty-nine of the 1280 primer combinations tested revealed polymorphism between the 2 extreme bulks. Further testing of these primer combinations in individual plants identified 5 AFLP markers tightly linked to the Rf gene with a map distance of less than 5.0 cM. All 5 markers were on one side of the restoration gene in the coupling phase. The closest marker, EA02MG03-260, is only 0.4 cM from the Rf gene. The EA02MG03-260 marker was converted to a dominant sequence characterised amplified region (SCAR) marker (SCARE2M3-214). Amplification using this locus-specific primer generated specific bands with male fertile plants when tested using the mapping population. Specific amplification of SCARE2M3-214 was also detected in all 3 male sterile plants and their F1 hybrids, with 5 restorer lines used for verification. Thus, SCARE2M3-214 will be very useful for the development of new restorer lines by the transfer of the Rf gene into other breeding lines. It can also be used for isolating the Rf gene by means of map-based cloning.

Download Full-text

Genetics Analysis of Rf Gene in Chilli Pepper (Capsicum annuum L.)

International Journal of Environment and Climate Change ◽

10.9734/ijecc/2021/v11i230368 ◽

2021 ◽

pp. 124-130

Author(s):

Manoj Kumar N. ◽

Vinod K. Sharma ◽

V. R. Sharma ◽

Ajay K Pandav

Keyword(s):

Agricultural Research ◽

Fertility Restoration ◽

Indian Agricultural Research Institute ◽

Dominant Gene ◽

Nuclear Gene ◽

Sweet Pepper ◽

Hot Pepper ◽

Male Sterile ◽

Male Sterile Line ◽

F1 Hybrid

The inability to develop functional pollen is caused by cytoplasmic male sterility (CMS), a maternally inherited trait. Restorer-of-fertility (Rf), a nuclear gene, could cause normal pollen production in CMS plants, resulting in fertility in the plant. This paper aimed to study the inheritance of restoration of fertility traits in both Sweet pepper and Hot pepper. The study was conducted in the Indian agricultural research institute, Katrain regional station, India. Genetic analysis of fertility restoration was performed on the progeny of chilli and sweet pepper. KTCA 5 (cytoplasmic-genetic male sterile line-Sweet pepper), KTCA 10 (cytoplasmic-genetic male sterile line-Sweet pepper. F2 segregation population and back cross BC1 population obtained from an F1 hybrid between KTCR 15 (a fertility restorer line). The fertility of the test-crossed lines was assessed under open field conditions using pollen related criteria. The fertility restoration trait segregated in 3:1 and 1:1 F2 segregation populations and backcrossed BC1 populations respectively in both Sweet Pepper and Hot pepper backgrounds. This indicates single dominant gene inheritance of the Rfgene. There is no effect of CMS background on the restoration trait inheritance.

Download Full-text

Cosegregation of Single Genes Associated with Fertility Restoration and Transcript Processing of Sorghum Mitochondrial orf107 and urf209

Genetics ◽

10.1093/genetics/150.1.383 ◽

1998 ◽

Vol 150 (1) ◽

pp. 383-391 ◽

Cited By ~ 1

Author(s):

Hoang V Tang ◽

Ruying Chang ◽

Daryl R Pring

Keyword(s):

Fertility Restoration ◽

Mitochondrial Gene ◽

Pollen Viability ◽

Single Gene ◽

Dominant Gene ◽

Nuclear Gene ◽

Male Sterile ◽

Reading Frame ◽

Transcript Processing ◽

Backcross Populations

Abstract Defective nuclear-cytoplasmic interactions leading to aberrant microgametogenesis in sorghum carrying the IS1112C male-sterile cytoplasm occur very late in pollen maturation. Amelioration of this condition, the restoration of pollen viability, involves a novel two-gene gametophytic system, wherein genes designated Rf3 and Rf4 are required for viability of individual gametes. Rf3 is tightly linked to, or represents, a single gene that regulates a transcript processing activity that cleaves transcriptsof orf107, a chimeric mitochondrial open reading frame specific to IS1112C. The mitochondrial gene urf 209 is also subject to nucleus-specific enhanced transcript processing, 5′ to the gene, conferred by a single dominant gene designated Mmt1. Examinations of transcript patterns in F2 and two backcross populations indicated cosegregation of the augmented orf107 and urf209 processing activities in IS1112C. Several sorghum lines that do not restore fertility or confer orf107 transcript processing do exhibit urf209 transcript processing, indicating that the activities are distinguishable. We conclude that the nuclear gene(s) conferring enhanced orf107 and urf209 processing activities are tightly linked in IS1112C. Alternatively, the similarity in apparent regulatory action of the genes may indicate allelic differences wherein the IS1112C Rf3 allele may differ from alleles of maintainer lines by the capability to regulate both orf107 and urf209 processing activities.

Download Full-text