scholarly journals Genetic identification and restriction fragment length polymorphism (RFLP) based analysis of Myxovirus resistant gene (Mx) of chicken in Bangladesh

2020 ◽  
Vol 20 (1-2) ◽  
pp. 18-25
Author(s):  
J Alam ◽  
M Hasan ◽  
K Hasan ◽  
M Giasuddin ◽  
MH Rahman ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Genetic Identification ◽  
Resistant Gene ◽  
Pcr Product ◽  
Allelic Gene ◽  
Restriction Fragment Length

The Myxovirus resistant gene (Mx) codes for Mx protein with antiviral activity. Mx gene has been found in yeast and vertebrates ranging from fish to humans. In the present study Mx gene and its diversity in chicken is reported. A total of 42 blood samples were collected from different groups of chicken and were analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The diversity of Mx gene was enumerated by RFLP of PCR product with RsaI and SspI restriction enzyme. Mx gene was amplified from all the tested samples (100%) with the primers used. The primers amplified specific 100bp and 300bp DNA fragments from Mx gene. RFLP analysis of PCR product obtained with mismatched primers revealed that three types of Mx gene allele are prevailing in the tested samples; these are homozygous resistant Mx allelic gene (R/R), heterozygous Mx allelic gene (R/S) and homozygous sensitive Mx allelic gene (S/S). The proportions of each gene allele in the sampled chicken were 42.86% R/R, 42.86% S/S and 14.29% R/S. Interestingly, variation in Mx gene was observed within and between the groups of chicken. Present study has limitations like number of sample, species of bird sampled, etc. However, the study provides the genetic basis to some extent of chicken about resistance or sensitivity to avian influenza. To the best of our knowledge this is the first report of Mx gene related study in chicken in Bangladesh. Bangladesh J. of Livestock Res. 20(1-2): 18-25, Jan-Dec 2013

Detection of Crustacean DNA and Species Identification Using a PCR–Restriction Fragment Length Polymorphism Method

2005 ◽  
Vol 68 (9) ◽  
pp. 1866-1873 ◽  
Author(s):  
JENNIFER L. BRZEZINSKI
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Food Products ◽  
Rrna Gene ◽  
Pcr Product ◽  
Allergenic Proteins ◽  
Restriction Fragment Length

The detection of potentially allergenic proteins, such as those derived from crustaceans, in food products is a major concern for the food processing industry. A PCR–restriction fragment length polymorphism (PCR-RFLP) method was designed to detect the presence of crustacean DNA in food products and to determine the species source of the DNA. This PCR assay amplifies an approximately 205-bp fragment of the 16S rRNA gene in crustacean species, including shrimp, crab, lobster, and crawfish. This reaction will not amplify DNA derived from mammals, such as cow and sheep. After amplification, the PCR product is digested with differential restriction endonucleases to determine the species source of the crustacean DNA. The specificity of this assay was demonstrated using four species of shrimp, three species of crab, and two species of lobster and crawfish. This assay is sensitive enough to detect crustacean DNA in a raw meat mixture containing <0.1% shrimp.

scholarly journals Identification of Polymorphisms of Gene CSN2 of B Casein in Greek Cow Breeds (Holstein) by Restriction Fragment Length Polymorphism

2020 ◽  
Vol 12 (11) ◽  
pp. 32
Author(s):  
Despina Vougiouklaki ◽  
Dionysios Antonopoulos ◽  
Stella Allexeli ◽  
Dimitra Houhoula
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Total Population ◽  
Fragment Length Polymorphism ◽  
Blood Samples ◽  
Pcr Product ◽  
Beta Casein ◽  
Restriction Fragment Length

The research focused to detect and identify genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows. For this purpose, DNA was extracted from 120 blood samples of cows. The desired region of exon 7 was amplified by PCR, resulting in a 121 bp product. The PCR product was digested by restriction fragment length polymorphism (RFLP) method. The results suggest that the A1A2 genotype prevails over the others. Specifically, of the 120 cattle, 72 showed triple bands of 121 bp, 86 bp, and 35 bp indicating the A1A2 genotype. The 42 cattle showed a single band at 121 bp, indicating that they carried the A1A1 genotype. The remaining 6 showed only two bands of 86 and 35 bps, indicating that they carried the A2A2 genotype. In the total population of heterozygotes A1A2-0.60 were the most frequent, while homozygotes A2A2-0.06 were the least frequent ones. This suggests a slight superiority of allele A-0.65.

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