scholarly journals In vitro regeneration and molecular characterization of some varieties of Lycopersicon esculentum Mill. in Bangladesh

2019 ◽  
Vol 54 (2) ◽  
pp. 117-124
Author(s):  
M Billah ◽  
TA Banu ◽  
M Islam ◽  
NA Banu ◽  
S Khan ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Molecular Characterization ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill ◽  
Regeneration Response

An efficient regeneration protocol was established for two varieties (BARI tomato-9 and BARI tomato-15) of tomato (Lycopersicon esculentum Mill.) using three explants namely cotyledonary node, cotyledonary leaf and hypocotyls. Among the three explants, maximum number of shoots was produced from cotyledonary leaf explants of BARI tomato-15 on MS with 2.0 mg/l BAP and 0.5 mg/l IAA. In this combination of BAP and IAA 86%, on an average, cotyledonary leaf explants showed regeneration response 14.12 shoots/explants. Explants from hypocotyl showed best results in MS medium with 2.0 mg/l BAP and 0.2 mg/l IAA in both the varieties. In case of cotyledonary node, BARI tomato-15 showed 6.0 shoot/explant on MS with 2.0 mg/l BAP and 1.0 mg/l IAA. Molecular characterization of total ten varieties of tomato in Bangladesh was done by using six arbitrary oligonucleotide RAPD primers. A total of 140 bands were produced where the highest genetic distance (0.6769) was found between BARI tomato-3 and Mintoo tomato and lowest distance (0.1035) was observed between BARI tomato-7 and BARI tomato-8. This result will be useful for designing future breeding programs. Bangladesh J. Sci. Ind. Res.54(2), 117-124, 2019

scholarly journals In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Tomato (Lycopersicon esculentum Mill.)

1970 ◽  
Vol 19 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Rakha Hari Sarker ◽  
Khaleda Islam ◽  
M.I. Hoque
Keyword(s):  
Lycopersicon Esculentum ◽  
Genetic Transformation ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill

Agrobacterium-mediated genetic transformation system has been developed for two tomato (Lycopersicon esculentum Mill.) varieties, namely Pusa Ruby (PR) and BARI Tomato-3 (BT-3). Prior to the establishment of transformation protocol cotyledonary leaf explants from the two varieties were cultured to obtain genotype independent in vitro regeneration. Healthy multiple shoot regeneration was obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength of MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants. Transformation ability of cotyledonary leaf explants was tested with Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121, containing GUS and npt II genes. Transformed cotyledonary leaf explants were found to produce multiple shoots on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin to 200 mg/l since kanamycin resistant gene was used for transformation experiments. Shoots that survived under selection pressure were subjected to rooting. Transformed rooted plantlets were transferred to soil. Stable expression of GUS gene was detected in the various tissues from putatively transformed plantlets using GUS histochemical assay.  Key words: In vitro regeneration, transformation, tomato D.O.I. 10.3329/ptcb.v19i1.5004 Plant Tissue Cult. & Biotech. 19(1): 101-111, 2009 (June)

IN VITRO REGENERATION PLANTS OF TWO CULTIVATED TOMATO LYCOPERSICON ESCULENTUM MILL.

2007 ◽  
pp. 67-71
Author(s):  
H. Majoul ◽  
S. Gharsallah-Chouchane ◽  
F. Gorsane ◽  
H. Fakhfakh ◽  
R. Lengliz ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
In Vitro Regeneration ◽  
Lycopersicon Esculentum Mill

Molecular characterization of a WEE1 gene homologue in tomato (Lycopersicon esculentum Mill.)

2004 ◽  
Vol 56 (6) ◽  
pp. 849-861 ◽  
Author(s):  
Nathalie Gonzalez ◽  
Michel Hernould ◽  
Fr�d�ric Delmas ◽  
Fr�d�ric G�vaudant ◽  
Philippe Duffe ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Molecular Characterization ◽  
Lycopersicon Esculentum Mill ◽  
Gene Homologue

scholarly journals In vitro regeneration and molecular characterization of Jatropha curcas plant

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mohamed El-Sayed ◽  
Usama I. Aly ◽  
Mervat S. Mohamed ◽  
Mohamed R. Rady
Keyword(s):  
Molecular Characterization ◽  
Jatropha Curcas ◽  
In Vitro Regeneration

In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

2017 ◽  
Author(s):  
Shikha Sharma ◽  
Geetika Gambhir ◽  
D. K. Srivastava
Keyword(s):  
Pisum Sativum ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Shoot Elongation ◽  
Root Regeneration ◽  
Pisum Sativum L ◽  
Regenerated Plantlets

In vitro regeneration of pea explants (Pisum sativum L. var. ‘Lincon’) was done in 49 different combinations and concentrations of BAP, BAP and NAA, BAP and IBA, TDZ, TDZ and Adenine for shoot regeneration from hypocotyl, root, leaf and cotyledonary node. High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively. No shoot regeneration was obtained from leaf explants on any of the combination used. Shoot elongation was observed on the same medium used for shoot regeneration respectively.MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots. The plantlets were able to regenerate within 6-7 weeks. The regenerated plantlets were acclimatized in pre-sterilized cocopeat.

In vitro regeneration of chlorophyll chimeras in tomato (Lycopersicon esculentum)

1981 ◽  
Vol 59 (10) ◽  
pp. 1941-1943 ◽  
Author(s):  
S. Seeni ◽  
A. Gnanam
Keyword(s):  
Lycopersicon Esculentum ◽  
Agar Medium ◽  
In Vitro Regeneration ◽  
Lycopersicon Esculentum Mill ◽  
Murashige Skoog

Explants of hypocotyl, cotyledon, and hypocotyl-derived calli of homozygous (xa-2/xa-2) green, heterozygous (Xa-2/xa-2) yellow–green, and homozygous (Xa-2/Xa-2) albino seedlings of Lycopersicon esculentum Mill. were induced to regenerate plants in vitro on Murashige-Skoog agar medium. Approximately 10–15% of the regenerants obtained from cultures of heterozygous individuals were of the variegated type. Homozygous lines green (xa-2/xa-2) and albino (Xa-2/Xa-2) however, did not regenerate any chimeral plants.

scholarly journals Genetic Transformation of a Local Tomato (Solanum lycopersicum L.) Variety of Bangladesh

2015 ◽  
Vol 25 (1) ◽  
pp. 87-97
Author(s):  
Pronabananda Das ◽  
Aneesa Ansari ◽  
Mohammad Nurul Islam ◽  
RH Sarker
Keyword(s):  
Genetic Transformation ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Bacterial Suspension ◽  
Marker Genes ◽  
Root Induction ◽  
Multiple Shoot Induction ◽  
Tomato Variety ◽  
Cotyledonary Leaf

An in vitro regeneration and Agrobacterium?mediated genetic transformation protocol was optimized for a local tomato variety, BARI Tomato?8 using cotyledonary leaf and hypocotyls explants. The explants were treated with various growth regulators in MS at different concentrations and combinations. Highest number of multiple shoot induction was observed from both the explants cultured in MS supplemented with 8.88 ?M BAP and 0.57 ?M IAA. Half strength of MS supplemented with 1.14 ?M IAA was found to be the best for root induction from excised shoots. Agrobacterium mediated genetic transformation was carried using pBI121 plasmid harboring ??glucuronidase (GUS) reporter and nptII selectable marker genes. Transient GUS assay confirmed that both the explants pre?cultured for two days showed best transformation efficiency in bacterial suspension having optical density (OD) of 0.8 (at 600 nm) for 15 min and co?cultivation period of 3 days. The shoots regenerated from transformed cotyledonary leaf explants survived at 200 mg/l kanamycin selection. The presence of expected amplicon corresponding to the GUS gene was confirmed by PCR. This protocol paves a way for developing disease resistant tomato variety using target gene/s.Plant Tissue Cult. & Biotech. 25(1): 87-97, 2015 (June)

In vitro regeneration and cytological characterization of shoots from leaf explants of three accessions of Solanum commersonii

1993 ◽  
Vol 34 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Teodoro Cardi ◽  
Vittoria Iannamico ◽  
Fabrizio D'Ambrosio ◽  
Edgardo Filippone ◽  
Paul F. Lurquin
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Solanum Commersonii
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