In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

2017 ◽  
Author(s):  
Shikha Sharma ◽  
Geetika Gambhir ◽  
D. K. Srivastava
Keyword(s):  
Pisum Sativum ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Shoot Elongation ◽  
Root Regeneration ◽  
Pisum Sativum L ◽  
Regenerated Plantlets

In vitro regeneration of pea explants (Pisum sativum L. var. ‘Lincon’) was done in 49 different combinations and concentrations of BAP, BAP and NAA, BAP and IBA, TDZ, TDZ and Adenine for shoot regeneration from hypocotyl, root, leaf and cotyledonary node. High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively. No shoot regeneration was obtained from leaf explants on any of the combination used. Shoot elongation was observed on the same medium used for shoot regeneration respectively.MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots. The plantlets were able to regenerate within 6-7 weeks. The regenerated plantlets were acclimatized in pre-sterilized cocopeat.

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid

2012 ◽  
Vol 47 (2) ◽  
pp. 211-216
Author(s):  
KK Paul ◽  
MA Bari
Keyword(s):  
Shoot Regeneration ◽  
White Light ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Multiple Shoot Regeneration ◽  
Regenerated Plantlets ◽  
Light Green

An efficient in vitro regeneration protocol was developed in medicinal aroid, Ghetkachu (Typhonium trilobatum Schott) using field grown corm bud explant. Highest percentage (75 %) of direct multiple shoot regeneration obtained in MS media supplemented with 5.0 mgL-1BAP + 1.5mg L-1NAA. Callus formation occur (80 %) in MS media containing 0.5mgL-1BAP + 2.0mgL-1NAA. The appearance of calli was white, creamy white light green in colour and the texture of calli were soft, friable and semi hard and compact. Shoot regeneration (85 %) obtained from calli in MS medium having 5.0mgL-1BAP +1.0mgL-1NAA. The regenerated plantlets were successfully acclimatized with loamy fertile soil and survived cent percentage in natural condition.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11454   Bangladesh J. Sci. Ind. Res. 47(2), 211-216, 2012  

scholarly journals In vitro Direct Regeneration from Node and Leaf Explants of Phalaenopsis cv. ‘Surabaya’

2016 ◽  
Vol 25 (2) ◽  
pp. 193-205 ◽  
Author(s):  
Khosro Balilashaki ◽  
Maryam Vahedi ◽  
Roghayeh Karimi
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Nodal Explants ◽  
Direct Regeneration ◽  
Flower Stalk ◽  
Breeding Programs ◽  
Half Strength ◽  
Direct Embryogenesis ◽  
Regenerated Plantlets

An efficient and reproducible procedure for the direct regeneration of phalaenopsis cv. ‘Surabaya’ using of nodal explants and leaf segments derived from in vitro flower stalk was conducted. Three experiments were carried out for shoot development and subsequent plant regeneration: Direct shoot regeneration from nodal explants of Phalaenopsis cv. ‘Surabaya’ flower stalks on MS added with different combination of NAA and BAP, direct regeneration of protocormlike bodies (PLBs) from leaf explants in a MS with different concentrations of the TDZ, acclimatization of regenerated plantlets in different mixture of components and nutrients. The results showed that 5 mg/l BAP and 2 mg/l NAA were most effective concentration for shoot regeneration, regenerated shoots were cultured on half strength of MS containing activated charcoal, IAA and NAA at various concentrations, highest number of root (6.7) was obtained in higher concentration of NAA (2 mg/l). TDZ induced a higher frequency of embryogenesis from leaf explants than BAP, the highest number of embryos per explant was 22.45 at 3 mg/l TDZ. Altogether, BAP at higher concentration (10 mg/l) with 1 mg/l NAA had the highest enhancement on the amount of direct embryogenesis. In our investigation 87.20% plantlets via nodal explants survived acclimatization process in medium containing cocopeat and coal (1 : 1). The survival rate of regenerated plantlets via nodal explants (82.07%) was more than of regenerated plantlets via leaf explants (70.47). This protocol provides the basis for further investigation on micropropagation and breeding programs in Phalaenopsis cv. ‘Surabaya’.Plant Tissue Cult. & Biotech. 25(2): 193-205, 2015 (December)

An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis

2013 ◽  
Vol 23 (2) ◽  
pp. 184-189 ◽  
Author(s):  
Alok Das ◽  
Sumit Kumar ◽  
P. Nandeesha ◽  
Indu Singh Yadav ◽  
Jyoti Saini ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Pisum Sativum L

scholarly journals In Vitro Shoot Regeneration and Polyploid Induction from Leaves of Hypericum Species

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1957-1961 ◽  
Author(s):  
Elisabeth M. Meyer ◽  
Darren H. Touchell ◽  
Thomas G. Ranney
Keyword(s):  
Shoot Regeneration ◽  
Chromosome Doubling ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Induction ◽  
Bluish Green ◽  
Spontaneous Chromosome ◽  
Ornamental Traits ◽  
In Vitro Shoot Regeneration

Hypericum L. H2003-004-016 is a complex hybrid among Hypericum frondosum Michx., Hypericum galioides Lam., and Hypericum kalmianum L. and exhibits valuable ornamental characteristics, including compact habit, bluish green foliage, and showy flowers. Inducing polyploidy may further enhance the ornamental traits of this hybrid and provide new opportunities for hybridizing with other naturally occurring polyploid Hypericum sp. In this study, in vitro shoot regeneration and treatment of regenerative callus with the dinitroaniline herbicide oryzalin (3,5-dinitro-N4,N4-dipropylsufanilamide) were investigated as a means of inducing allopolyploidy. First, in vitro regeneration was optimized for callus and shoot induction by culture of leaf explants on medium supplemented with benzylamino purine (BA) or meta-topolin (mT) at 5, 10, or 15 μM in combination with indoleacetic acid (IAA) at 0, 1.25, 2.5, or 5 μM. Both BA and mT treatments successfully induced regenerative callus and shoots. Multiple regression analysis estimated maximum regenerative callus (94%) and shoot induction (18 shoots per explant) in medium supplemented with 5 μM BA and 3.75 μM IAA. In the second part of the study, exposure of regenerative callus to oryzalin at 0, 7.5, 15, 30, 60, or 90 μM for durations of 3, 6, or 9 d was investigated for polyploid induction. There was no survival for any of the calli in the 60- or 90-μM oryzalin treatments, but calli subjected to the other treatments exhibited some survival and polyploid induction. Duration had no effect on callus survival or ploidy level, but oryzalin concentration was a significant factor in both. The greatest percentage (44%) of polyploids was induced with 30 μM oryzalin. Spontaneous chromosome doubling was observed in 8% of control explants receiving no oryzalin treatment.

The influential role of polyamines on the in vitro regeneration of pea (Pisum sativum L.) and genetic fidelity assessment by SCoT and RAPD markers

2019 ◽  
Vol 139 (3) ◽  
pp. 547-561 ◽  
Author(s):  
Chandrasekaran Ajithan ◽  
Venkatachalam Vasudevan ◽  
Dorairaj Sathish ◽  
Selvam Sathish ◽  
Veda Krishnan ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Rapd Markers ◽  
In Vitro Regeneration ◽  
Genetic Fidelity ◽  
Pisum Sativum L ◽  
Fidelity Assessment

scholarly journals Pea (Pisum sativum L.) in biology prior and after Mendel's discovery

2014 ◽  
Vol 50 (No. 2) ◽  
pp. 52-64 ◽  
Author(s):  
P. Smýkal
Keyword(s):  
Pisum Sativum ◽  
Reverse Genetics ◽  
Agronomic Traits ◽  
In Vitro Regeneration ◽  
Lotus Japonicus ◽  
Model Organism ◽  
Germplasm Collections ◽  
Economic Significance ◽  
Pisum Sativum L

Pea (Pisum sativum L.) has been extensively used in early hybridization studies and it was the model organism of choice for Mendel’s discovery of the laws of inheritance, making pea part of the foundation of modern genetics. Pea has also been used as model for experimental morphology and physiology. However, subsequent progress in pea genomics has lagged behind many other plant species, largely as a consequence of its genome size and low economic significance. The availability of the genome sequences of five legume species (Medicago truncatula, Lotus japonicus, Glycine max, Cajanus cajan and Cicer aerietinum) offers opportunities for genome wide comparison. The combination of a candidate gene and synteny approach has allowed the identification of genes underlying agronomically important traits such as virus resistances and plant architecture. Useful genomic resources already exist and include several types of molecular marker sets as well as both transcriptome and proteome datasets. The advent of greater computational power and access to diverse germplasm collections enable the use of association mapping to identify genetic variation related to desirable agronomic traits. Current genomic knowledge and technologies can facilitate the allele mining for novel traits and their incorporation from wild Pisum sp. into elite domestic backgrounds. Fast neutron and targeting-induced local lesions in genomes (TILLING) pea mutant populations are available for reverse genetics approaches, BAC libraries for positional gene cloning as well as transgenic and in vitro regeneration for proof of function through gene silencing or over-expression. Finally, recently formed International Pea Genome Sequencing Consortium, holds promise to provide the pea genome sequence by 2015, a year of 150 anniversary of Mendel’s work.

scholarly journals In vitro regeneration and molecular characterization of some varieties of Lycopersicon esculentum Mill. in Bangladesh

2019 ◽  
Vol 54 (2) ◽  
pp. 117-124
Author(s):  
M Billah ◽  
TA Banu ◽  
M Islam ◽  
NA Banu ◽  
S Khan ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Molecular Characterization ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill ◽  
Regeneration Response

An efficient regeneration protocol was established for two varieties (BARI tomato-9 and BARI tomato-15) of tomato (Lycopersicon esculentum Mill.) using three explants namely cotyledonary node, cotyledonary leaf and hypocotyls. Among the three explants, maximum number of shoots was produced from cotyledonary leaf explants of BARI tomato-15 on MS with 2.0 mg/l BAP and 0.5 mg/l IAA. In this combination of BAP and IAA 86%, on an average, cotyledonary leaf explants showed regeneration response 14.12 shoots/explants. Explants from hypocotyl showed best results in MS medium with 2.0 mg/l BAP and 0.2 mg/l IAA in both the varieties. In case of cotyledonary node, BARI tomato-15 showed 6.0 shoot/explant on MS with 2.0 mg/l BAP and 1.0 mg/l IAA. Molecular characterization of total ten varieties of tomato in Bangladesh was done by using six arbitrary oligonucleotide RAPD primers. A total of 140 bands were produced where the highest genetic distance (0.6769) was found between BARI tomato-3 and Mintoo tomato and lowest distance (0.1035) was observed between BARI tomato-7 and BARI tomato-8. This result will be useful for designing future breeding programs. Bangladesh J. Sci. Ind. Res.54(2), 117-124, 2019

Development of an efficient protocol for high frequency in vitro regeneration of a horticultural plant Primulina tamiana (B.L. Burtt) Mich. Möller & A. Webber

2014 ◽  
Vol 94 (7) ◽  
pp. 1281-1287 ◽  
Author(s):  
Priya Padmanabhan ◽  
Susan J. Murch ◽  
J. Alan Sullivan ◽  
Praveen K. Saxena
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
De Novo ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Root Induction ◽  
Horticultural Plant ◽  
Leaf Segments

Padmanabhan, P., Murch, S. J., Sullivan, J. A. and Saxena, P. K. 2014. Development of an efficient protocol for high frequency in vitro regeneration of a horticultural plant Primulina tamiana (B.L. Burtt) Mich. Möller & A. Webber. Can. J. Plant Sci. 94: 1281–1287. A rapid and efficient in vitro regeneration system was established for Primulina tamiana, an attractive ornamental plant of horticultural importance. The successful regeneration protocol employed induction of shoot organogenesis on leaf explants. Among the various plant growth regulators evaluated, thidiazuron (TDZ) proved to be the most effective in inducing rapid de novo shoot formation. The highest shoot regeneration frequency within the shortest time from leaf explants was obtained on medium enriched with 2.5 µM TDZ. Furthermore, leaf segments were found to be a more suitable explant for in vitro shoot regeneration compared with petiole explants. The abaxial side of the leaf segments, which was in contact with the culture medium, generated more shoots than the adaxial side. Scanning electron microscopic studies carried out at various stages of de novo shoot differentiation demonstrated that shoot organogenesis involved the formation of actively dividing regions from the epidermal and subepidermal layers of the explants. In vitro rooting of the regenerated shoots was attempted using two different auxins, IAA (3-indoleacetic acid) and 3-indolebutyric acid (IBA) (0, 0.1, 0.5, 1.0, 2.0, and 5.0 µM). The optimum concentration for root induction was found to be 5 µM IBA. Approximately, 98% of the regenerated plants survived transfer and acclimation to greenhouse conditions.

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