scholarly journals Influence of Growth Regulators on Shoot Proliferation and Plantlet Production from Shoot Tips of Banana

2013 ◽  
Vol 20 (1-2) ◽  
pp. 9-16
Author(s):  
S Rehana ◽  
MS Alam ◽  
KS Islam ◽  
MA Samad
Keyword(s):  
Root Formation ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Lower Percentage ◽  
Ms Medium ◽  
Plantlet Production ◽  
Single Shoot

The effect of BAP and IBA on in vitro regeneration of four banana cultivars viz. ‘Amritsagar’, ‘Seeded banana’, ‘Sabri’ and ‘Anajee’ was studied. The response of single shoot regeneration from shoot tips of four banana cultivars at different concentrations of BAP was found to be different. The cultivar ‘Amritsagar’ produced the highest percentage (60 %) of single shoot at 4.0 mg/l BAP within 10-15 days. The cultivar ‘Sabri’ and ‘Anajee’ produced lower percentage of single shoots. Rates of shoot multiplication of ‘Amritsagar’, ‘Sabri’, and ‘Anajee’ were 6-7 plantlets/explant, 2-4 plantlets/explant, and 2 plantlets/explant, respectively on medium containing 4.0 mg/l BAP after 30 days of culture. But in subsequent subculture, on the same medium, ‘Amritsagar’ produced the highest number of plantlets (9 plantlets/explant) within the same period of time. The best root formation in multiplied shoots of ‘Amritsagar’ was found on MS medium containing 2.0 mg/l IBA after 15 days of culture. All the in vitro cultured banana plantlets of ‘Amritsagar’ survived when weaned to ex vitro conditions on soil.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16840 Progress. Agric. 20(1 & 2): 9 – 16, 2009

In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

2020 ◽  
Vol 100 (2) ◽  
pp. 202-208
Author(s):  
Mengting Wang ◽  
Guiliang Zhang ◽  
Peiyao Xin ◽  
Yun Liu ◽  
Bin Li ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Germplasm Conservation ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

scholarly journals Regeneration of plants from alginate-encapsulated shoots of Rhododendron dalhousiae Hook. F.

2011 ◽  
Vol 3 (1) ◽  
pp. 29-33 ◽  
Author(s):  
K. K. Singh ◽  
Bhusan Gurung
Keyword(s):  
Root Formation ◽  
Solid Medium ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Synthetic Seed ◽  
Root Induction ◽  
Ms Medium ◽  
Direct Sowing

A method has been developed for plant regeneration from alginate-encapsulated nodal segments of Rhododendron dalhousiae. Shoot tips collected from in vitro proliferated shoots were used for synthetic seed production. For encapsulation, nodal segments were mixed with MS medium supplemented with 3% sodium alginate and incubated with calcium chloride (60 mM). The maximum frequency (69%) of conversion of encapsulated shoot tips into plantlets was achieved on MS medium containing 25 μM 2-isopentenyladenine (2iP) along with additive such as, 100 mg L-l polyvinyl pyrrolidone (PVP), 100 mg L -l ascorbic acid, 10 mg L-l citric acid. The presence of 2iP (25 μM) with IAA (0.6 μM) improved re-generation. Amongst the two gelling agents used higher shoot proliferation as well as better growth were observed in cultures grown on Agar in comparison to Phytagel medium. Encapsulated nodal segments stored at 4°C for 25 days also showed successful conversion, followed by development into complete plantlets when returned to regeneration medium. Liquid medium was superior over solid medium for root formation and growth. IBA (1.0 μM) was more effective than other auxins for root induction. Plantlets with developed shoot and roots were hardened off to survive ex vitro conditions and successfully established in greenhouse. Possibility of direct sowing of synthetic seeds in the soil was also examined.

scholarly journals In vitro propagation of Citrullus lanatus Thumb. from nodal explants culture

1970 ◽  
Vol 8 (2) ◽  
pp. 203-206 ◽  
Author(s):  
MM Khatun ◽  
MS Hossain ◽  
MA Haque ◽  
M Khalekuzzaman
Keyword(s):  
Citrullus Lanatus ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Nodal Explant ◽  
Grown Plant

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully. Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus DOI: 10.3329/jbau.v8i2.7926 J. Bangladesh Agril. Univ. 8(2): 203-206, 2010  

scholarly journals Micropropagation of Clerodendrum phlomidis L.F.

2016 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
Neha Srivastava ◽  
Murugan Nataraj ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Axillary Shoot Proliferation ◽  
Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

scholarly journals Ethnobotany and In vitro regeneration of Acorus calamus L. (Acoraceae): a high valued medicinal and economic plant

2017 ◽  
Vol 6 (2) ◽  
pp. 1566
Author(s):  
Jintu Sarma* ◽  
Pratibha Sharma
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Culture Technique ◽  
Axillary Bud ◽  
Cow Dung ◽  
Acorus Calamus ◽  
Economic Plant ◽  
Ms Medium ◽  
Important Medicinal Plant

Acorus calamus L. is a species of enormous medicinal and economic importance. In vitro propagation of this plant was achieved using axillary bud explant. In the present investigation, naturally grown axillary bud and rhizome explants were cultured on standard MS and B5 medium supplemented with different concentration and combination of cytokinines and auxines. The best shoot proliferation was observed in MS medium containing Kn (1.0mg/l) +IBA (0.5mg/l) with 3.33±0.58 nos. of Shoots, 7.33±0.58 nos. of roots and 15.33±0.58 nos. leaves. In B5 medium best results found in Kn (1.5mg/) + NAA (1.0mg/l) with 2.67±0.58 nos. shoots, 3.67±0.58 nos. of roots and11.67±0.58 nos. of leaves. They were then transplanted in soil: sand: cow dung mixture (1:1:2) and kept in shade for 4 to 5 weeks and then transferred to field for one month. Survival rate was found 80 % in MS medium and 100 % in B5 medium. The present investigation was carried out with a view to standardize an in vitro culture technique for mass propagation of this important medicinal plant species and was found successful.

scholarly journals Influence of the Genotype and TDZ on the in Vitro Regeneration of Hybrid Grapes

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 877B-877
Author(s):  
Maritza I. Tapia ◽  
Paul E Read
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Ms Medium ◽  
High Concentrations ◽  
Grape Cultivars

It has been previously demonstrated that thidiazuron (TDZ) enhanced the regeneration and multiple shoot proliferation of vinifera grape cultivars. To determine the effect of TDZ on the multiplication of hybrid grapes, in vitro nodal segments from cultivars Chancellor, Leon Millot, and Valiant were cultured on MS medium supplemented with 0, 0.01, 0.05, 0.1, 0.5, and 1.0 mg TDZ/liter. After 1 month, the higher percentage of rooted shoots was obtained from the explants cultured in medium containing the lowest concentration of TDZ (0.01 mg–liter–1) independent of the genotype. Multiple shoot proliferation was favored by high concentrations of TDZ (0.5 and 1.0 mg–liter–1). An average of 0.39 and 0.39 shoots, respectively, was obtained from `Chancellor' cultures, 0.56 and 0.59 from `Leon Millot', and 1.93 and 2.38 from `Valiant'. Vitrification and teratological structures were observed in all the cultures of the three genotypes, but less vitrification occurred in `Valiant' plantlets.

scholarly journals Long-term in Vitro Regeneration of Hamelia patens

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873G-874
Author(s):  
D. Sankhla ◽  
T.D. Davis ◽  
N. Sankhla ◽  
A. Upadhyaya
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Prolific Shoot ◽  
Ornamental Shrub

This report describes an efficient in vitro regeneration protocol for H. patens (firebush), a heat-tolerant ornamental shrub native to tropical and subtropical America. Shoot cultures were initially established using shoot tips placed on MS-revised medium containing 2.3 μM 2,4-D, 2.3 μM kinetin, and 0.25% polyvinylpyrrolidone. Other types of explants (nodal and internodal segments, leaf pieces, floral buds) did not regenerate shoots when placed on this medium. Two-month-old plantlets derived from the shoot tips were subcultured on MS medium supplemented with 0.5 μM thidiazuron (TDZ), and within 3 to 4 weeks, some callus was produced at the root–shoot junction. When this callus, with a small portion of the root and shoots, was placed on MS medium with 0.05 μM TDZ and 0.01 μM ABA, prolific shoot formation occurred within 3 to 4 weeks followed by root formation. By regular subculturing every 5 to 6 weeks, hundreds of plantlets have been obtained over the past 3 years with no apparent decline in regeneration potential. Addition of activated charcoal (0.5%) to the culture medium has greatly improved growth of the plantlets.

scholarly journals Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Mohammed Elsayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Yougasphree Naidoo
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Naphthalene Acetic Acid ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Protocol ◽  
Regenerated Plantlets

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

scholarly journals Proliferation-rate Effects of BAP and Kinetin on Banana (Musa spp. AAA Group) ‘Basrai’

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1253-1255 ◽  
Author(s):  
Aish Muhammad ◽  
Hamid Rashid ◽  
Iqbal Hussain ◽  
S.M. Saqlan Naqvi
Keyword(s):  
Liquid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Shoot Height ◽  
Significant Difference ◽  
Rate Effects ◽  
Single Shoot ◽  
Number Of Shoots

The effect of benzylaminopurine (BAP) and kinetin alone and in combination with indole 3-acetic acid (IAA) on shoot proliferation of ‘Basrai’ (Musa spp., AAA group) was investigated. Shoot tips (4–6 mm) were excised from field-grown suckers to initiate the cultures. Concentrations of BAP and kinetin ranged from 0.0 to 8.0 mg·L−1 each on solid or in liquid MS medium. When liquid medium was used, cultures were agitated continuously on an orbital shaker moving at 40 rpm. Three subculture regimes were employed; after each subculture, the number of shoots regenerated from each explant was counted. The results showed that the multiplication rate was significantly (P ≤ 0.05) dependent upon cytokinin type, its concentration, and type of medium used. The maximum number of shoots regenerated from a single shoot tip was achieved in liquid MS medium containing 4.0 mg·L−1 BAP. There was no significant difference between liquid and solid medium when kinetin was used; however, kinetin at 4.0 mg·L−1 or above yielded significant results as compared with the control and lower kinetin concentrations. The results demonstrated that 4.0 mg·L−1 BAP with 1.0 mg·L−1 IAA in liquid medium was best for shoot multiplication and shoot height during micropropagation of ‘Basrai’.

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

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