Biorational management of maize fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) using Bacillus thuringiensis (Berliner) enriched with chemical additives

An invasive pest, fall armyworm, Spodoptera frugiperda (J.E.Smith) (Lepidoptera: Noctuidae) attacks maize at every stage of development, from seedling emergence up to cob formation. Early instar larvae were seen mostly on leaves of maize with characteristics pin or shot hole symptoms. Later instar larvae were confined to deep whorls, leaving typically ragged like appearance and fed on the reproductive stage of the crop especially tassels and developing cobs resulting in quality and quantity loss of maize produce. The effect of commercially available Bacillus thuringiensis subsp. kurstaki product, Dipel® against the second instar larvae of Fall Armyworm (FAW )was not promising under laboratory conditions. Hence, an effort was made to add an adjuvant along with B. thuringiensis to increase the virulence of commercially available B. thuringiensis.The Laboratory bioassays with B. thuringiensis and seven chemical additives ( T1- Bt + Boric acid, T2- Bt + Zinc oxide, T3- Bt + Sodium nitrate, T4- Bt + Peptone, T5- Bt + Urea, T6- Bt + EDTA, T7- Bt + Citric acid & T8- Bt alone T9- Control) were tested against second instar larvae of Spodoptera frugiperda larvae. The results showed that B. thuringiensis plus sodium nitrate (T3) promoted maximum mortality 82.2 per cent with a minimum LC50 value of 54.620 mg/l. Sodium nitrate boosted B. thuringiensis activity at a concentration of 0.05 per cent by 2.128-fold than B. thuringiensis alone. Overall, sodium nitrate improved the efficacy of B. thuringiensis spray at the maximum level followed by boric acid, urea, EDTA and peptone.

Effect of biochar composite and organic sources on soil properties and yield of bhendi (Abelmoschus esculentus L.)

Biochar is considered as a possible and potential tool for soil fertility improvement, climate change mitigation and long term sink for atmospheric carbon dioxide. Soil application of biochar enhances the soil properties indirectly. A field experiment was conducted to evaluate the influence of organic manures viz., Farm Yard Manure (FYM), Vermicompost, Biochar, Biochar composite on soil properties, growth and yield of bhendi, Abelmoschus esculentus in Somayyanur soil series of Madurai district, Tamil Nadu. The experiment was laid out in randomized block design (RBD) with eleven treatments and three replications during the summer season (March – June) 2021 with the inclusion of inorganic fertilizers based on soil test crop response (STCR) based recommendation. Application of biochar composite (5 t ha-1) along with STCR based NPK (75% STCR) increased the total carbon content in soil by 0.538 %. This, in turn, increased the available nitrogen status to 295 and 244 Kg ha-1 at 40 and 70 DAS, respectively. Similarly, the available phosphorous (22.4, 19.3 Kg ha-1) and potassium (344.70, 323.70 Kg ha-1) status also showed a considerable increase with the same treatment. The yield attributes of bhendi viz., fruit length, girth, weight, dry matter production and yield recorded maximum values of 15.23 cm, 6.93 cm, 21.56 g, 11.9 t ha-1 and 25.20 t ha-1 with the combined application of biochar composite and NPK. The findings revealed that 25 % STCR based NPK could be reduced with the application of 5 t ha-1 of biochar composite, which is economically an option besides promoting soil health.

Effect of long term fertilization on phosphorus dynamics in root zone environment under finger millet - Maize cropping sequence

In an agro-ecosystem, phosphorus (P) is found in organic and an inorganic form which includes soluble P, sorbed P and mineral bounded P. In soil, added P sources undergo various soil processes like mineralization, immobilization, precipitation, weathering, dissolution, sorption and desorption. For understanding the P dynamics in clay loam (Vertic Ustropept) soil, the present study was undertaken in P dynamics under rhizosphere and non-rhizosphere environment of maize in Long Term Fertilizer Experiment at Tamil Nadu Agricultural University, Coimbatore. The results revealed that the fractions of various pools of inorganic NaOH extractable Fe - P, H2SO4 extractable Ca- P, NH4F extractable Al- P, NH4Cl extractable Saloid P and Na citrate - Dithionate extractable Reductant soluble P were dominant in the non-rhizospheric soil than rhizospheric environment. The order of inorganic P fractions in the non-rhizospheric and rhizospheric region of the soil was found as Ca-P > Fe-P > Al-P > Reductant soluble-P > Saloid P and the knee-high stage of the non - rhizosphere soil recorded the highest inorganic as well organic P fractions. Irrespective of P fractions, Ca – P was recorded high (192.5 & 186.7 mg kg-1 ) followed by Fe - P (40.8& 34.9 mg kg-1) at a knee-high stage in non-rhizosphere and rhizosphere, respectively. Practising various nutrient management systems, application of 100% recommended dose of fertilizer along with FYM @ 10 t ha-1 (T8) recorded significant changes in all inorganic (Ca-P, Fe-P, Al-P, Reductant soluble-P, Saloid P), organic fractions and also Total P followed by 150% NPK (T3) in sandy clay loam soil. Nowadays, increasing demand for P fertilizer in India, judicious use of P fertilizer is important. Despite that, intensively cultivated soils have a lot of P reserves like organic and inorganic P pools and effective way of P transformation management could reduce the quantum of P fertilization in soil.

Phytostimulation and growth promotion activities of Trichoderma spp. on groundnut (Arachis hypogaea L.) crop

Groundnut (Arachis hypogaea L.) suffers from many soil borne pathogens that deteriorate the quality of the seeds and are responsible for high yield loss. Practically Trichoderma sp. is used for seed treatment, it minimizes the seed and soil borne pathogens and supports plant growth promotion activities. In the present study, five different isolates of Trichoderma spp. were isolated from groundnut (A. hypogaea ) rhizosphere soil. All the five isolates were confirmed by morphological methods and using molecular tools through Polymerase Chain Reaction (PCR) amplification of Internal Transcribed Spacer (ITS) region of Trichoderma sp. and DNA gets amplified in 650 bp to 700 bp. Trichoderma spp. were molecularly identified as T(SP)-20 (Trichoderma longibrachiatum), T(AR)-10 (T. asperellum), T(VT)-3 (T. hamatum), T(BI)-16 (T. longibrachiatum), T(TK)-23 (T. citrinoviride). Phytostimulation activities of all the six isolates viz., phosphate solubilization, Ammonia production, IAA production, and Siderophore production, were evaluated. Among the six isolates, T(SP)-20, T(AR)-10, and TNAU-TA showed higher phytostimulation activities. The growth promotion of Trichoderma spp. on groundnut was assessed through the roll towel method. The isolate T(SP)-20 (T. longibrachiatum) produced the highest germination percentage of 93.33 and vigor index of 2246.2. This work developed a new isolate of T. longibrachiatum (T(SP)-20) which is a native isolate having significant phytostimulation and growth promotion activities and it could be exploited for other soil borne disease managing successfully.

Standardization of optimum sieve size for maximizing seed quality in Amaranthus (Amaranthus tricolor L.)

Vegetables have a critical function in human health and nutritional security. Vegetables are considered an essential building block of any diet. Out of the leafy vegetables, Amaranthus is the most popular and salable vegetable consumed by people all over India. Seed processing experiment was undertaken in Amaranthus tricolor (CO 2) by using the sieves placed inside the mechanical seed shaker (Gyratory sieve shaker) to improve the quality of Amaranthus seeds. The seeds of Amaranthus were size graded with seed shaker attached with various sieve size of BSS 18 X18 (R), BSS 20 X 20 (R), BSS 22 X 22 (R) and BSP 22 X 22 (P). During processing, the machine could be adjusted for 2, 3, 4 and 5 minutes with an oscillating speed of 1440 rpm. The separated seeds were evaluated for seed quality characteristics such as seed germination percentage, shoot length, root length, dry matter production, 1000 seed weight and seed recovery percentage. The results revealed that the sieve size of BSS 22 X 22 (R=retained) mesh sieve had the better quality seeds with a maximum recovery of 67.41 g and when it is operated for a period of 5 minutes. The germination percentage was improved from 77 % to 95 % with 1000 seed weight of 73.21 mg, and the observed recovery was 56 per cent with the vigour index of 1145. Hence, BSS 22 X 22 retained mesh sieve with a duration of 5 minutes could be recommended as an optimum sieve size for grading Amaranthus seeds for improving the seed quality.

Crosstalk between circadian rhythms, sleep and eating habits to improve public health

Lifestyle changes in the past few decades have resulted in irregularity in sleep and meal timings. People are taking less sleep and resorting more to unhealthy food and eating habits. These factors are the most important health determinants. Sleep, food choices and eating habits are closely associated with each other. Thus, the crosstalk between circadian rhythms, sleep, and food needs urgent attention and discussion for better health management. Various research studies indicate that a healthy trend in sleep augments the regularity in meal timings and good eating habits. Healthy food and eating habits, in turn, boost sleep quality. Deterioration in the quality of anyone adversely affects the other. Several health disorders like obesity, diabetes, cancer, cardiovascular problems, and low immunity can be linked to these disturbances. The present review considers several previous studies to point out the inter-relationship between sleep and eating habits and the various health disorders arising from poor attention. Poor sleep promotes the tendency to eat junk food or eat at odd times, such as snacking late at night, leading to weight gain and other health problems. Junk food and unhealthy eating habits cause sleep disturbances. This inter-relationship of sleep and food preferences is important as it can have important treatment implications for health disorders arising due to these modifiable behavioural factors. Circadian alignment, improvements in sleep timings, and healthy eating habits have positive effects on other health behaviours as well.

Extrapolation of post-harvest soil test values in barnyard millet-based cropping sequence through multivariate analysis

The soil test value is based on the soil test-based fertilizer prescription/ recommendation equation. Each crop harvesting after the next crop is necessary to analyze the soil. Therefore, it is necessary to develop an alternative technique to predict postharvest soil tests after the harvest of every crop. For that a study was conducted in mixed black calcareous soils at Tamil Nadu agricultural University, Coimbatore to develop the post-harvest prediction equations for available nitrogen, phosphorus and potassium in barnyard millet cropping sequence based on a multiple regression model by considering post-harvest soil test value as the dependent variable and initial available nutrients, fertilizer doses and crop yield or crop nutrient uptake as an independent variables. The developed model was validated by computing R2 value, RMSE (root means square error), RE (relative error), and the ratio of performance to deviation (RPD) and the developed model was found to be valid. Using the validated model, post-harvest soil test values were predicted. A fertilizer recommendation was made for blackgram based on predicted post-harvest soil test values in the barnyard millet-blackgram cropping sequence. The predicted soil test values were compared with actual soil test values and it revealed that the developed model is fairly accurate and best-fitted with more precision. The predicted post-harvest soil test values of barnyard millet could be used in order to prescribe fertilizer for desired yield targets for subsequent crops.

Smartphone assist deep neural network model to recognize the high-quality tea using leaf maturity and its effect on leaf chlorophyll

Immature and tender tea leaves always produce high-quality tea than mature tea leaves. Depending on the maturity and age of the leaf, the colour and texture of the tea leaf are different. The photosynthesis capacity of the tea leaf also changes with the change of leaf maturity. Though the tea farmer plucks, classifies, and recognizes the best tea leaves (immature and tender) by viewing the visual symptoms and position of the leaves, the method is not authentic all time and leads to the overall degradation of the tea quality. The present study presents a smartphone assist tea leaf recognition system by analyzing the colour and texture properties of the tea leaf. The six different colour features and 4 Haralick texture features were extracted in the colour and grey domain of the leaf images. Three types of tea leaves, i.e., mature, immature, and tender, were classified using Deep Neural Network (DNN) with ADAM (Adaptive Moment Estimation) optimizer. With an accuracy of 97%, the DNN outperformed the Support Vector Machine (SVM) and K Nearest Neighbor (KNN). The SVM and KNN reported a total of 94.42% and 95.53% accuracy, respectively. The investigated system using DNN with an average precision and recall value of 98.67 and 98.34, respectively, may detect and classify the tea leaf maturity status. The system also can be used in AI-based tea plucking robotic systems or machines.

Response of growth, yield and quality of small onion (Allium cepa L. var. aggregatum don.) to Tamil Nadu Agricultural University-Water Soluble Fertilizers (TNAU-WSF)

Enhancing the food production for the growing world population has needed application of highly sustainable and efficient inputs to produce more food per unit of land. Hence, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu has produced Water soluble fertilizers (WSF) in its maiden attempt and it is necessary to optimize on different crops. Small onion is one of the most important vegetables in the Indian diet and it has high demand but low productivity. To enhance crop productivity and quality of small onions, the application of TNAU-WSF was taken up. A field experiment was laid out in a Randomized block design (RBD) incorporating 8 treatments comprising of application of RDF at100% NPK as TNAU WSF, soil test based application of 75%, 100%, 125% NPK ha-1 as TNAU-WSF with soil application of sulphur (S) and foliar spray of TNAU Liquid multi micronutrient (LMM) and without S and TNAU LMM and absolute control. Each treatment was replicated thrice with onion (CO 4). Soil test based application of 125% NPK ha-1 as TNAU-WSF with sulphur (S) and TNAU LMM recorded significantly higher in plant height (54.01 cm), the number of leaves per bulb (8.56), leaf greenness (67.5 SPAD), root length (5.42 cm), polar bulb diameter (4.38 cm), equatorial bulb diameter (2.72 cm) fresh bulb weight (74.21 g), bulb yield (1751 t ha-1) and quality attributes like total soluble solids (TSS) (14.78 °Brix), ascorbic acid content (15.34 mg 100 g-1), pyruvic acid content (2.27 µmol g-1). However, soil test based application of 100% NPK ha-1 as TNAU-WSF was found to be an ideal rate to attain the economic target yield of the onion crop.

Effect of packaging material and postharvest calcium treatment on weight loss, decay and biochemical quality of strawberry fruits during storage

Strawberry (Fragaria x ananassa Duch.) fruits are highly perishable which lose their marketability within a short period of time, resulting in considerable loss of fruits after harvest. In present study, the packaging materials treatment i.e. polypropylene terephthalate (PET) Punnet and High density polythene (HDPE) crates and calcium compounds (CaCl2 and Ca(No3)2) were examined for the storability and biochemical quality of strawberry (cv. Winter Down) fruits in cold condition (3±1 °C) in comparison to the untreated control fruit in ambient storage for 3, 6 and 8 days storage period. Results revealed that physiological loss in weight (PLW) and fruit decay increased with the advancement of the storage period irrespective of the treatments. Fruits treated with CaCl2 (2%), packed in PET Punnets and stored in cold condition (3±1 °C) showed significantly minimum PLW (6.55, 11.12 and 15 34 %) and fruit decay (7.29, 17.71 and 34.38 %) at 3, 6 and 8 days storage, respectively. Non-treated control fruits at ambient conditions could not maintain their marketability even 3 days of storage and showed relatively high decay losses (25.00, 62.21 and 91.67 % at 3, 6 and 8 days of storage, respectively). Packaging and calcium treatment did not affect the fruit total soluble solids (TSS) and total sugars but was found effective to slow down the decrease of ascorbic acid and acidity contents of the fruit during storage. PET Punnets packaged fruits treated with CaCl2 (2%) under cold conditions retained better shelf-life with better biochemical quality of fruits during storage while untreated control fruits deteriorated rapidly.