scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Rapid in vitro micropropagation of Cicer arietinum L.

2008 ◽  
Vol 34 (No. 1) ◽  
pp. 1-5 ◽  
Author(s):  
G. Sujatha ◽  
N. Jayabalan ◽  
D. Ranjitha Kumari B
Keyword(s):  
Cicer Arietinum ◽  
Multiple Shoots ◽  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Indole Butyric Acid ◽  
Shoot Number

A rapid, simple and efficient protocol for <i>in vitro</i> multiple shoot induction and plantlet regeneration was achieved from three different explants of <i>Cicer arietinum</i>. The explants viz shoot tip, cotyledonary node and node were cultured on MS medium fortified with Benzyl Adenine (BA) (0.44&minus;8.88&mu;M) for multiple shoot induction. Multiple shoots proliferation was best observed at 4.44&mu;M BA from all the three explants within two weeks of culture. Of the three different explants tested, cotyledonary nodes produced the maximum number of shoots. Shoot number per explant ranged between 7 and 15. Individual shoots were aseptically excised and subcultured in the same media for shoot elongation. The elongated shoots were transferred to Indole Butyric Acid (IBA) (2.46&minus;12.30&mu;M) for root induction. Rooting was observed within two weeks of culture. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 76.3%. Plants looked healthy with no visually detectable phenotypic variations.

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes

2015 ◽  
Vol 41 ◽  
pp. 71-77
Author(s):  
S. Parvin ◽  
M. Kausar ◽  
M. Enamul Haque ◽  
M. Khalekuzzaman ◽  
B. Sikdar ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Ms Medium ◽  
Cotyledonary Nodes ◽  
Cucumis Melo L

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from invitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growthregulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.Effective result was found on MS medium supplemented with 2.0 mg/l BAP, inwhich 90% and 70% cultures induced multiple shoots from nodal segments andshoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodeswere found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS mediumsupplemented with different concentrations of IBA and NAA for successful rootinduction and the effective result (up to 70%) was found in medium with 1 mg/lIBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, whereas 70% plantlets survived

In vitro propagation and multiple shoot induction of Rhodiola rosea L. by axillary bud culture

2012 ◽  
Vol 39 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Kee-Hwa Bae ◽  
Myung-Suk Ko ◽  
Nam-Young Kim ◽  
Jae-Mo Song ◽  
Gwan-Pil Song
Keyword(s):  
In Vitro Propagation ◽  
Multiple Shoot ◽  
Rhodiola Rosea ◽  
Axillary Bud ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Axillary Bud Culture

scholarly journals Micropropagation of Centella asiatica L. An Important Medicinal Herb

2013 ◽  
Vol 19 (2) ◽  
pp. 51-56 ◽  
Author(s):  
R Das ◽  
MF Hasan ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
In Vitro Propagation ◽  
Centella Asiatica ◽  
Multiple Shoot ◽  
Medicinal Herb ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
High Frequencies ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants

High frequencies of multiple shoot regeneration were achieved from shoot tip explants cultured on MS media fortified with 4.0 mgl-1 BAP + 0.1 mgl-1 NAA. On an average 10.2?0.38 shoots per explant were obtained. The regenerated shoots were rooted in MS medium supplemented with 1.0 mgl-1 IBA. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival. A reproducible protocol was established for in vitro propagation through multiple shoot induction of Centella asiatica L. an important medicinal herb having high medicinal value.DOI: http://dx.doi.org/10.3329/pa.v19i2.16928 Progress. Agric. 19(2): 51 - 56, 2008

scholarly journals An improved micropropagation protocol for lentisk (Pistacia lentiscus L.)

2019 ◽  
Vol 31 (1) ◽  
pp. 61-69
Author(s):  
Hakan Yildirim ◽  
Ahmet Onay ◽  
Kazim Gunduz ◽  
Sezai Ercisli ◽  
Firat Ege Karaat
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Shoot Length ◽  
Pistacia Lentiscus ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Long Shoots

AbstractThis study presents an efficient improvement in the in vitro propagation protocol for one cloned genotype of lentisk (Pistacia lentiscus L.) by assessing the effects of gibberellic acid (GA3) concentrations, different cytokinins and amino acids and their concentrations on shoot proliferation, the effects of shoot length on rooting and the effects of compost type (sterile and non-sterile) on acclimatization. The best growth medium for multiple shoot induction was the MS medium supplemented with a combination of 1 mg l−1 BA, 100 mg l−1 tryptophan and 0.5 mg l-1 GA3, which gave a mean shoot length of 1.64 ± 0.07 cm and a mean bud number of 5.46 ± 0.16. The best results in terms of root length, rooting rate and the number of roots per shoot were obtained with 2 cm long shoots. The rooted plantlets were readily acclimatized in the sterile compost. In conclusion, the micropropagation protocol developed in this study can be used for large-scale propagation of P. lentiscus L. in reforestation programmes.

scholarly journals Multiple Shoot Induction from Radicle-derived Callus and in Vitro Propagation of Silene Acaulis Subsp. Arctics

2006 ◽  
Vol 33 (4) ◽  
pp. 303-307
Author(s):  
Hyo-Won Seo ◽  
Jung-Yoon Yi ◽  
Young-Eun Park ◽  
Sung-Ho Kang ◽  
Ho-Sung Chung ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Silene Acaulis

scholarly journals IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD

2016 ◽  
Vol 4 (12SE) ◽  
pp. 23-28
Author(s):  
Ayyadurai V ◽  
Ramar K
Keyword(s):  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Solanum Pubescens

Efficientin Vitro direct multiple shoot regeneration from Solanum pubescens was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 1.0mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible protocol useful the mass propagation of Solanum pubescens.

IN VITRO CALLUS INDUCTION IN PHARMACOLOGICALLY SIGNIFICANT HERB: ECLIPTA ALBA

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
PUSHPLATA DODAKE ◽  
MALIKA PAL
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Growth Period ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
In Vitro Plant Regeneration ◽  
Multiple Shoot Induction ◽  
Eclipta Alba ◽  
Nodal Tissue

A competent protocol was developed for in vitro plant regeneration and multiplication through callus culture of Eclipta alba. Perfect medium for callus establishment through nodal tissue was observed in 2,4-D supplemented media. The callus formed was creamish yellow in colour, showed a growth period of three to four weeks. A moderate quality callus was obtained with different gradients of BAP supplemented medium. A white to creamy callus with rapid growth was observed in the medium supplemented with NAA. Callus was subjected for multiple shoot induction.

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