IN VITRO CALLUS INDUCTION IN PHARMACOLOGICALLY SIGNIFICANT HERB: ECLIPTA ALBA

A competent protocol was developed for in vitro plant regeneration and multiplication through callus culture of Eclipta alba. Perfect medium for callus establishment through nodal tissue was observed in 2,4-D supplemented media. The callus formed was creamish yellow in colour, showed a growth period of three to four weeks. A moderate quality callus was obtained with different gradients of BAP supplemented medium. A white to creamy callus with rapid growth was observed in the medium supplemented with NAA. Callus was subjected for multiple shoot induction.

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In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i1.5970 ◽

2010 ◽

Vol 20 (1) ◽

pp. 73-79 ◽

Cited By ~ 2

Author(s):

M. F. Hasan ◽

B. Sikdar

Keyword(s):

Plant Regeneration ◽

In Vitro Propagation ◽

Multiple Shoots ◽

Multiple Shoot ◽

Shoot Tips ◽

Root Induction ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words: Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm

National Academy Science Letters ◽

10.1007/s40009-018-0714-2 ◽

2018 ◽

Vol 42 (3) ◽

pp. 271-276

Author(s):

Akshay Joshi ◽

Subhash Deokule

Keyword(s):

Plant Regeneration ◽

Multiple Shoot ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Ephedra Foliata

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Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v25i1.24110 ◽

2015 ◽

Vol 25 (1) ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Kee Hwa Bae ◽

Eui Soo Yoon

Keyword(s):

Plant Regeneration ◽

Seed Germination ◽

Callus Induction ◽

In Vitro Propagation ◽

Ornamental Plants ◽

Leaf Explants ◽

Adventitious Shoot ◽

Temperature Treatment ◽

In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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