Optimization of sterile male ratio of oriental fruit fly, Bactrocera dorsalis (HENDEL)

The study was conducted to establish the Sterile Insect Technique (SIT) method of controlling oriental fruit fly, Bactrocera dorsalis (Hendel). The sterile male ratio of oriental fruit fly was optimized for possible field application of Sterile Insect Technique. Several batches of 5 and 6-dayold pupae were irradiated at 30, 40, 50 and 60 Gray (Gy) to optimize the radiation dose of sterilization. Highest pupal duration and abnormal adults were found after imposing gamma radiation in 5-day-old pupae treated with 60 Gy dose. Radiation significantly increased the nonemergence percentage and decreased the normal emergence of oriental fruit fly. The sterilizing doses were recorded as 60 Gy for 5-dayold pupae. Fixed number of virgin females of oriental fruit flies were allowed to mate with unirradiated and irradiated males at 1:1, 1:2, 1:5 and 1:9 ratios in different cages to find out the rate of suppression against wild population. Minimum pupal recovery and percent normal emergence observed at 1:9 ratio. Deformed and undeveloped (not emerged) population increased with the ratio of irradiated males. The 1:9 ratio was found as the best for suppression of wild population of B. dorsalis.SAARC J. Agri., 13(2): 50-60 (2015)

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Effect of Irradiating Puparia of Oriental Fruit Fly (Diptera: Tephritidae) on Adult Survival and Fecundity for Sterile Insect Technique and Quarantine Purposes

Journal of Economic Entomology ◽

10.1093/jee/toz217 ◽

2019 ◽

Vol 112 (6) ◽

pp. 2808-2816 ◽

Cited By ~ 1

Author(s):

Suhana Yusof ◽

Ahmad Zainuri Mohamad Dzomir ◽

Salmah Yaakop

Keyword(s):

Sex Ratio ◽

Sterile Insect Technique ◽

Adult Emergence ◽

Fruit Fly ◽

Bactrocera Dorsalis ◽

Adult Survival ◽

Adult Longevity ◽

Oriental Fruit Fly ◽

New Findings ◽

Morphological Differences

Abstract The aim of this study was to investigate the effect of gamma irradiation on survivability (adult emergence, sex ratio, adult longevity), fecundity (eggs hatchability, number of eggs produced), and morphological differences in the size of the ovary and testes of unirradiated and irradiated adults of Oriental fruit fly [Bactrocera dorsalis (Hendel)]. A dose of 100 Gy was determined as the minimum needed for inhibitory effects against B. dorsalis that would not deter adult emergence, sex ratio (male:female), and adult longevity, with 82.6 ±7.02, 1:1.09, and 107 ± 24.5, respectively. Doses from 50 to 400 Gy range did not completely prevent the adult emergence; however, the emergence decreased by increasing the radiation dose. Adult survivability significantly decreased among all the treatment groups of B. dorsalis, except for the 50 Gy and unirradiated flies. At a dose of 50 Gy, fertile females showed a significant reduction in fecundity by not producing eggs after mating with the sterile males. Meanwhile, the number of eggs laid decreased with increasing dose and no fertile egg was hatched starting at 100 Gy of irradiation. Testes and ovaries of 20-d old flies irradiated as pupae were smaller than those of control flies. In this study, 100 Gy was concluded as the minimum effective dose for the disinfestation and sterilization of B. dorsalis puparia. Results represent new findings used as a basis for sterile insect technique and quarantine programs for managing B. dorsalis, particularly in Malaysia.

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Polyandry in the Fruit Fly Bactrocera dorsalis Hendel (Diptera: Tephritidae)

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.61108 ◽

2021 ◽

Vol 24 (2) ◽

pp. 209

Author(s):

Defiana Prastiti ◽

Suputa Suputa ◽

Y. Andi Trisyono

Keyword(s):

Mating Behavior ◽

Sterile Insect Technique ◽

Multiple Mating ◽

Fruit Fly ◽

Fruit Flies ◽

Bactrocera Dorsalis ◽

Oriental Fruit Fly ◽

Comprehensive Understanding ◽

Mated Female

The Oriental fruit fly, Bactrocera dorsalis (Hendel) is a global pest of a wide variety fruits. Due to its importance, the sterile insect technique (SIT) has raised attention as a safe and sustainable solution to this pest. Successful SIT programs require a comprehensive understanding on the mating behavior of this species. Females of oriental fruit flies are known monandrous which implies that females only mate once with one male. This experiment aimed to confirm that female flies were able to mate more than once with the same (multiple mating) or different males (polyandry) in certain period of time. Four different experiments were conducted to test the hypothesis: receptivity a female to the same male (1) and different males (2) in a cage for 11 days; receptivity a mated female with the same male (3) and different males (4) in a cage for 14 days after the first mating. Results indicated that a part of the female oriental fruit flies was capable to mating more than once with the same or different males.

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