Bacteria-targeted infection imaging: Towards a faster diagnosis of bacterial infection

In order to seek novel technetium-99m bacterial infection imaging agents, a ciprofloxacin xanthate (CPF2XT) was synthesized and radiolabeled with [99mTcN]2+ core to obtain the 99mTcN-CPF2XT complex, which exhibited high radiochemical purity, hydrophilicity, and good stability in vitro. The bacteria binding assay indicated that 99mTcN-CPF2XT had specificity to bacteria. A study of biodistribution in mice showed that 99mTcN-CPF2XT had a higher uptake in bacterial infection tissues than in turpentine-induced abscesses, indicating that it could distinguish bacterial infection from sterile inflammation. Compared to 99mTcN-CPFXDTC, the abscess/blood and abscess/muscle ratios of 99mTcN-CPF2XT were higher and the uptakes of 99mTcN-CPF2XT in the liver and lung were obviously decreased. The results suggested that 99mTcN-CPF2XT would be a potential bacterial infection imaging agent.

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Radiolabeling and Evaluation of a Novel [TcN]2 Complex with Deferoxamine Dithiocarbamate as a Potential Agent for Bacterial Infection Imaging

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2021.128102 ◽

2021 ◽

pp. 128102

Author(s):

Yuhao Jiang ◽

Si'an Fang ◽

Xuran Zhang ◽

Junhong Feng ◽

Qing Ruan ◽

...

Keyword(s):

Bacterial Infection ◽

Infection Imaging

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Synthesis ofN4′-[18F]Fluoroalkylated Ciprofloxacin as a Potential Bacterial Infection Imaging Agent for PET Study

Bioconjugate Chemistry ◽

10.1021/bc1002983 ◽

2010 ◽

Vol 21 (12) ◽

pp. 2282-2288 ◽

Cited By ~ 17

Author(s):

Kalme Sachin ◽

Eun-Mi Kim ◽

Su-Jin Cheong ◽

Hwan-Jeong Jeong ◽

Seok Tae Lim ◽

...

Keyword(s):

Bacterial Infection ◽

Imaging Agent ◽

Infection Imaging

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Synthesis and Biodistribution of a Novel99mTcN Complex of Norfloxacin Dithiocarbamate as a Potential Agent for Bacterial Infection Imaging

Bioconjugate Chemistry ◽

10.1021/bc100357w ◽

2011 ◽

Vol 22 (3) ◽

pp. 369-375 ◽

Cited By ~ 20

Author(s):

Shijian Zhang ◽

Weifang Zhang ◽

Yue Wang ◽

Zhonghui Jin ◽

Xuebin Wang ◽

...

Keyword(s):

Bacterial Infection ◽

Infection Imaging

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Synthesis and Evaluation of Novel Norfloxacin Isonitrile 99mTc Complexes as Potential Bacterial Infection Imaging Agents

Pharmaceutics ◽

10.3390/pharmaceutics13040518 ◽

2021 ◽

Vol 13 (4) ◽

pp. 518

Author(s):

Si’an Fang ◽

Yuhao Jiang ◽

Di Xiao ◽

Xuran Zhang ◽

Qianqian Gan ◽

...

Keyword(s):

Bacterial Infection ◽

Single Photon ◽

Photon Emission ◽

Sterile Inflammation ◽

Imaging Agents ◽

Emission Computed Tomography ◽

Infection Imaging ◽

Spect Image ◽

Biodistribution Studies

To develop potential technetium-99m single-photon emission computed tomography (SPECT) imaging agents for bacterial infection imaging, the novel norfloxacin isonitrile derivatives CN4NF and CN5NF were synthesized and radiolabeled with a [99mTc][Tc(I)]+ core to obtain [99mTc]Tc-CN4NF and [99mTc]Tc-CN5NF. These compounds were produced in high radiolabeling yields and showed hydrophilicity and good stability in vitro. The bacterial binding assay indicated that [99mTc]Tc-CN4NF and [99mTc]Tc-CN5NF were specific to bacteria. Compared with [99mTc]Tc-CN4NF, biodistribution studies of [99mTc]Tc-CN5NF showed a higher uptake in bacteria-infected tissues than in turpentine-induced abscesses, indicating that [99mTc]Tc-CN5NF could distinguish bacterial infection from sterile inflammation. In addition, [99mTc]Tc-CN5NF had higher abscess/blood and abscess/muscle ratios. SPECT image of [99mTc]Tc-CN5NF showed that there was a clear accumulation in the infection site, suggesting that it could be a potential bacterial infection imaging radiotracer.

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