Features of algae tests of samples containing dissolved organic matter

2020 ◽  
pp. 40-45
Author(s):  
Elena V. Fedoseeva ◽  
Iya Yu. Grigorieva ◽  
Olga V. Nikolaeva ◽  
Vera A. Terekhova
Keyword(s):  
Organic Matter ◽  
Chlorophyll Fluorescence ◽  
Dissolved Organic Matter ◽  
Marine Algae ◽  
Cultivation Conditions ◽  
High Reproducibility ◽  
Test Culture ◽  
Production And Consumption ◽  
Number Of Cells ◽  
Consumption Waste

Algae tests are widely used due to the availability of cultivation conditions and high reproducibility. Freshwater and marine algae are used to estimate the toxicity of drinking, natural and wastewater, water extracts from soils, sewage sludge and production and consumption waste. The presence of dissolved organic matter in the samples, stimulating the growth of algae and the intensity of chlorophyll fluorescence, can significantly affect the result of algae tests, and therefore on the decision on the presence or absence of a toxic effect. When choosing algae as a test culture to determine toxic effects, it is necessary to take into account the possibility of hyperstimulating the number of cells with dissolved organic matter.

scholarly journals Advanced characterization of dissolved organic matter released by bloom-forming marine algae

2017 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Z.U. Rehman ◽  
S. Jeong ◽  
S.A.A. Tabatabai ◽  
A,-H. Emwas ◽  
T. Leiknes
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Marine Algae ◽  
Advanced Characterization

scholarly journals Response of Chlorophyll Fluorescence Characteristics and Dissolved Organic Matter for Marine Diatom Skeletonema dohrnii under Stress from Penicillin and Zn2+

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2684
Author(s):  
Yang Liu ◽  
Xiaofang Liu ◽  
Jun Sun
Keyword(s):  
Organic Matter ◽  
Chlorophyll Fluorescence ◽  
Dissolved Organic Matter ◽  
Environmental Stress ◽  
Metal Ion ◽  
Physiological State ◽  
Marine Diatom ◽  
Chlorophyll Fluorescence Characteristics ◽  
Marine Habitats ◽  
Fluorescence Characteristics

Skeletonema dohrnii is a good model diatom for studying environmental stress and has promising applications and prospects in various fields. Antibiotics and heavy metals are commonly exceeded in the nearshore marine habitats. In this work, we investigated the effects of an antibiotic (penicillin, 2 µg/L) and a heavy metal ion (Zn2+, 10 µmol/L) stress on marine diatom S. dohrnii, mainly using excitation-emission matrices (EEMs) fluorescence methods and OJIP test. Results indicated that algal cells grown with the antibiotic showed higher biomass, specific growth rate, doubling time, chlorophyll a, and chlorophyll fluorescence variables. Moreover, excess zinc had negative effects on S. dohrnii. We found that zinc not only inhibited the relative photosynthetic electron transfer efficiency but also reduced the Chl a content, which ultimately affected algal growth and organic matter production. In addition, the combined effect of penicillin and Zn2+ further affected the physiological state of S. dohrnii. The dissolved organic matter (DOM) characteristics of the four cultures were also different, including fluorescence indices (fluorescence index, biological index, β/α, and humification index) and fluorescence peaks (peaks A, C, M and T). In brief, characterization of chlorophyll fluorescence characteristics and DOM-related variables are important for understanding the effects of environmental stress on microalgae.

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